Genes associated with the release of dormant buds in tree peonies (Paeonia suffruticosa)

Plants must carefully regulate their development in order to survive a wide range of conditions. Of particular importance to this is dormancy release, deciding when to grow and when not to, given these varying conditions. In order to better understand the growth release mechanism of dormant tissue at the molecular and physiological levels, molecular markers can be used. One gene family that has a long association with dormancy, which is routinely used as a marker for dormancy release, is DRM1/ARP (dormancyassociated gene-1/auxin-repressed protein). This plant-specific gene family has high sequence identity at the protein level throughout several plant species, but its function in planta remains undetermined. This review brings together and critically analyzes findings on the DRM1/ARP family from a number of species. We focus on the relevance of this gene as a molecular marker for dormancy, raising questions of what its role might actually be in the plant.

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“…PsARP showed a sharp increase prior to bud break and then dropped to low levels as the buds grew [9].…”

Section: Drm1/arp and Dormancymentioning

confidence: 99%

The Dormancy MarkerDRM1/ARPAssociated with Dormancy but a Broader RoleIn Planta

Rae

David

Wood

2013

Developmental Biology Journal

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“…In addition, the transcript of PsMYB1 quickly increased after 21 days chilling requirement when transferred to greenhouse for 1 days, but reached peak after enduring 7 days chilling when transferred to greenhouse for 3 days. The buds that received more than 21 days chilling treatment entered the other physiological stage, eco-dormancy (Huang et al 2008a). Our results indicated that the response of PsMYB1 to temperature changes of complete dormancy release might be quicker than its response of dormancy flower buds, which suggested that PsMYB1 might be associated to eco-dormancy and budbreak.…”

Section: Discussionmentioning

confidence: 99%

“…In the past 10 years, studies on the tree peony mainly focused on the physiological and horticultural aspects of dormancy, such as nutrient substance, membrane lipid peroxidation and the endogenous hormone changes during the fulfillment of the chilling requirement (Liu et al 2004;Luo et al 2008;Zheng et al 2009). Huang et al (2008a) firstly screened 31 unigenes associated with dormancy release in the tree peony by suppression subtractive hybridization (SSH). But the majority of studies focused mainly on functional genes, with little focus on transcription factors (TF).…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression analysis of the R2R3-PsMYB1 gene associated with bud dormancy during chilling treatment in the tree peony (Paeonia suffruticosa)

Zhang

Gai

et al. 2014

Plant Growth Regul

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Transcription factor (TF) MYB-like proteins are involved in many physiological and biochemical processes. This study isolated the full length cDNA encoding a MYB from the tree peony (Paeonia suffruticosa Andr.), whose GenBank Accession No. is KC282466. The deduced MYB-like protein sequence shares extensive sequence similarity with previously characterized plant R2R3-MYBs, and was named PsMYB1 according to the accepted nomenclature for TFs. Phylogenetic analysis showed that the deduced amino acid of PsMYB1 was closely related to VvMYB30 from grape. The PsMYB1 gene was expressed in all tissues, but it was most strongly expressed in the stem, and least expressed in the root. When exposed to chilling at 4°C, PsMYB1 was up-regulated at 7 days and then decreased until 21 days suggesting it responded to low temperature, and it might negatively regulate dormancy release. In addition, the PsMYB1 quicker response to the temperature changes in flower buds of complete dormancy release might be that PsMYB1 involved in eco-dormancy and accelerated budbreak. These experiments enhance our knowledge of PsMYB1 and provide more information for understanding the genetic and physiological changes during the dormancy release process in the tree peony.

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“…High expression levels of ARP were also reported to be associated with dormant bud release in tree peonies (Paeonia suffruticosa; Huang et al 2008). As in tree peonies, the upregulation of ARP might promote sprouting in shortleaf pine after top removal.…”

Section: Hormone-related Genesmentioning

confidence: 97%

“…Recently, several microarray experiments have been carried out to profile genes responsible for dormancy release of buds of perennial trees and perennial grasses (Leida et al 2010;Huang et al 2008;Mazzitelli et al 2007;Pacey-Miller et al 2003). Normally, for a cDNA array, the cDNA clones printed on the slides and the labeled mRNA hybridized to it are from the same species, but researchers have utilized the so-called cross-hybridization.…”

Section: Introductionmentioning

confidence: 99%

Gene level responses of shortleaf pine and loblolly pine to top removal

Liu

Will

Tauer

2011

Tree Genetics & Genomes

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Young shortleaf pine sprouts prolifically after death of the stem due to top removal or fire damage. Considerable interest has been shown in shortleaf pine restoration due to continually declining native populations. Shortleaf pine's strong sprouting ability has tremendous potential in promoting its regeneration. However, little is known about its sprouting mechanisms at the molecular level. We designed a microarray experiment to study genes responsible for sprouting ability. In this study, 1-year-old shortleaf pine and loblolly pine seedlings' tops were removed mechanically and the remaining stem tissues collected just before sprouting. Shortleaf pine showed an extraordinarily strong sprouting ability. Large numbers of sprouts were seen within 2 days after top removal. However, loblolly pine developed only a few sprouts about 1 week following top removal. Using microarray gene profiling with about 2,400 cDNA clones obtained from suppression subtractive hybridization, 139 differentially expressed genes were found to be associated with the sprouting response, including genes functioning in reserve (carbohydrates, protein, and fatty acid) mobilization, transcriptional regulation, stress response, plant development, signal transduction, and hormone regulation. Shortleaf pine responds actively to top removal at the molecular level, while loblolly pine apparently does not. As reported for dormancy release of buds of other perennial plants, oxidative stress might be the major cause of dormancy release of axillary buds of shortleaf pine. Apparent cross talk between plant hormones (gibberellins, auxins, and ethylene), carbohydrates, and other players of signal transduction leads to the cooperative promotion of sprouting of shortleaf pine after top removal.

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Genes associated with the release of dormant buds in tree peonies (Paeonia suffruticosa)

Cited by 29 publications

References 41 publications

The Dormancy MarkerDRM1/ARPAssociated with Dormancy but a Broader RoleIn Planta

The Dormancy MarkerDRM1/ARPAssociated with Dormancy but a Broader RoleIn Planta

Cloning and expression analysis of the R2R3-PsMYB1 gene associated with bud dormancy during chilling treatment in the tree peony (Paeonia suffruticosa)

Gene level responses of shortleaf pine and loblolly pine to top removal

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