Genes coding for intermediate filament proteins closely related to the hagfish “thread keratins (TK)” α and γ also exist in lamprey, teleosts and amphibians

Schaffeld, Michael; Schultess, Jan

doi:10.1016/j.yexcr.2006.01.005

Cited by 32 publications

(28 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We also determine Young's modulus to be Y ≈ 9 MPa, which is consistent with previously reported values of the keratin-like IF proteins from hagfish slime threads. 47,48 Thus, the full nonlinear behavior of vimentin networks is well described by this theory for crosslinked networks of stretchable, semiflexible polymers.…”

Section: Resultsmentioning

confidence: 81%

Divalent Cations Crosslink Vimentin Intermediate Filament Tail Domains to Regulate Network Mechanics

Lin

Broedersz

Rowat

et al. 2010

Journal of Molecular Biology

114

154

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 81%

Divalent Cations Crosslink Vimentin Intermediate Filament Tail Domains to Regulate Network Mechanics

Lin

Broedersz

Rowat

et al. 2010

Journal of Molecular Biology

114

154

View full text Add to dashboard Cite

“…This provided major clues relating to keratin evolution in vertebrates, but also pointed to a more general role of this previously considered highly specific IF protein group in vertebrates [28]. By combination of RT-PCR experiments and cDNA library screening (for details see Methods), from sturgeon and gar we have now isolated one and from bichir two cDNA sequences that, according to our phylogenetic analysis, code for members of another novel keratin group (Fig.…”

Section: Resultsmentioning

confidence: 92%

“…4). Compared to our recent analyses [14-18,28], we have now included additional type I keratin sequences from zebrafish and Xenopus ; therefore, in this study the K18 branch shows a more complex branching pattern, not allowing a clear identification of the authentic K18 counterparts in the different vertebrates solely on the basis of their position in the tree. However, our previous identification of K18 in shark, bichir, sturgeon, trout and zebrafish was additionally based on its typical occurrence in simple epithelia, such as liver hepatocytes or intestinal mucosal epithelium, corresponding to the situation in man and other tetrapods [10,12-16,19,29].…”

Section: Resultsmentioning

confidence: 99%

A novel and ancient group of type I keratins with members in bichir, sturgeon and gar

et al. 2007

Self Cite

View full text Add to dashboard Cite

Abstract1. Background: Vertebrate epithelial cells typically express a specific set of keratins. In teleosts, keratins are also present in a variety of mesenchymal cells, which usually express vimentin. Significantly, our previous studies revealed that virtually all known teleost keratins evolved independently from those present in terrestrial vertebrates. To further elucidate the evolutionary scenario that led to the large variety of keratins and their complex expression patterns in present day teleosts, we have investigated their presence in bichir, sturgeon and gar. Results:We have discovered a novel group of type I keratins with members in all three of these ancient ray-finned fish, but apparently no counterparts are present in any other vertebrate class so far investigated, including the modern teleost fish. From sturgeon and gar we sequenced one and from bichir two members of this novel keratin group. By complementary keratin blot-binding assays and peptide mass fingerprinting using MALDI-TOF mass spectrometry, in sturgeon we were able to assign the sequence to a prominent protein spot, present exclusively in a two-dimensionally separated cytoskeletal preparation of skin, thus identifying it as an epidermally expressed type I keratin. In contrast to the other keratins we have so far sequenced from bichir, sturgeon and gar, these new sequences occupy a rather basal position within the phylogenetic tree of type I keratins, in a close vicinity to the keratins we previously cloned from river lamprey. Conclusion:Thus, this new K14 group seem to belong to a very ancient keratin branch, whose functional role has still to be further elucidated. Furthermore, the exclusive presence of this keratin group in bichir, sturgeon and gar points to the close phylogenetic relationship of these ray-finned fish, an issue still under debate among taxonomists.

show abstract

“…Actin filaments and intermediate filaments (keratins) play an important role in maintaining cellular integrity and structure (Ku et al, 1999). Keratins may also be involved in apoptosis and signal transduction, while actin has a role in cell-cell adherence junction and cell-cell communication (Schaffeld & Schultess, 2006). Tropomyosin is known to be important in morphogenesis and to promote microfilament stability (Gunning et al, 1997).…”

Section: Toxicological Effects Of Produced Water and Oil Dischargesmentioning

confidence: 99%

Integrative Environmental Genomics of Cod (Gadus morhua): The Proteomics Approach

Karlsen

Bjørneklett

Berg

et al. 2011

Journal of Toxicology and Environmental Health, Part A

View full text Add to dashboard Cite

Atlantic cod (Gadus morhua) is an essential species in North Atlantic fisheries and increasingly relevant as an aquaculture species. However, potential conflicts with both coastal industry and petroleum industry expanding into northern waters make it important to understand how effluents (produced water, pharmaceuticals, food contaminants, and feed contaminants) affect the growth, reproduction, and health of this species in order to maintain a sustainable cod population and a healthy human food source, and to discover biomarkers for environmental monitoring and risk assessment. The ongoing genome sequencing effort of Atlantic cod has opened the possibility for a systems biology approach to elucidate molecular mechanisms of toxicity. Our study aims to be a first step toward such a systems toxicology understanding of genomic responses to environmental insults. A toxicogenomic approach was initiated that is combining data generated from proteomics analyses and transcriptomics analyses, and the concurrent development of searchable expressed sequence tags (EST) databases and genomic databases. This interdisciplinary study may also open new possibilities of gene annotation and pathway analyses.

show abstract

Genes coding for intermediate filament proteins closely related to the hagfish “thread keratins (TK)” α and γ also exist in lamprey, teleosts and amphibians

Cited by 32 publications

References 55 publications

Divalent Cations Crosslink Vimentin Intermediate Filament Tail Domains to Regulate Network Mechanics

Divalent Cations Crosslink Vimentin Intermediate Filament Tail Domains to Regulate Network Mechanics

A novel and ancient group of type I keratins with members in bichir, sturgeon and gar

Integrative Environmental Genomics of Cod (Gadus morhua): The Proteomics Approach

Contact Info

Product

Resources

About