Genes de virulência e diversidade genética em Salmonella spp. isoladas de amostras de origem suína

Moura, Mariela Silva; Oliveira, Raquel Peres de; Melo, Roberta Torres de; Mendonça, Eliane Pereira; Fonseca, Belchiolina Beatriz; Rossi, D. A.

doi:10.1590/1678-6809

Cited by 8 publications

(6 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There were also signs of neglect of biosecurity standards in the food industry environment due to the presence of the agent in clean and dirty processing areas. According to Moura et al [ 43 ], this oversight is decisive in the maintenance of the microorganism in the environment. At the same time, pulsotype K indicated that there was spread of this profile, since there were similar strains in Mato Grosso do Sul and Minas Gerais, Brazil, in different slaughter units.…”

Section: Resultsmentioning

confidence: 99%

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

et al. 2021

View full text Add to dashboard Cite

The presence of virulence genes, phylogenetic relationships, biofilm formation index (BFI), and ultrastructure in S. Minnesota at different temperatures (4, 25, and 36 °C) were analyzed. In addition, the ability of biocidal agents (chlorhexidine1%, sodium hypochlorite 1%, and peracetic acid 0.8%) to inhibit biofilms formed by 20 strains isolated from broiler slaughter plants from two Brazilian companies in 2009, 2010, and 2014 was determined. The presence of specific genes was evaluated by PCR and phylogeny between strains by pulsed-field gel electrophoresis. The BFI was determined using tryptone soy broth with 5% of chicken juice, and its structure was observed by scanning electron microscopy. The presence of specific genes indicated that S. Minnesota has the potential to cause disease in humans, adapting to adverse conditions. Temperatures of 25 and 36 °C favored biofilm formation, although at 4 °C, there was still biomass that could contaminate the final product. Tolerance to all biocides was identified in 12/20 (60%), representing a real risk of adaptation mechanisms development, especially regarding to resistance to sodium hypochlorite. Phylogenetic analysis indicated cross-contamination and spread among companies, which was probably related to biofilms formation. Results show the necessity of attention to this serovar considering its resistance to sodium hypochlorite, including the need for rigorous control, adopting low temperatures to prevent biofilms formation in the poultry industry.

show abstract

Section: Resultsmentioning

confidence: 99%

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Therefore, it is necessary to establish more efficient and rigorous hygiene and biosafety measures to guarantee the control of this agent in the aviary's environment. According to Moura et al (2014) the neglecting of biosecurity standards within the industry is a decisive factor for the maintenance of the microorganism in the environment.…”

Section: Resultsmentioning

confidence: 99%

Characteristics of virulence, resistance and genetic diversity of strains of Salmonella Infantis isolated from broiler chicken in Brazil

et al. 2020

View full text Add to dashboard Cite

Salmonella Infantis is frequently associated with human infections worldwide and is transmitted by consumption of contaminated foods, particularly those of animal origin, especially the chicken meat. We aimed to evaluate virulence characteristics, antimicrobial resistance and the genetic similarity of 51 strains of S. Infantis isolated from samples of poultry origin. The strains were isolated from 2009 to 2010 in a company with full cycle of broiler’s production in the state of São Paulo, Brazil. The antimicrobial susceptibility test was performed and, by PCR, we evaluated the presence of the genes lpfA (hem-adhesion), agfA (hem-biofilm) and sefA (hem-adhesion) and resistance genes to beta-lactams (blaTEM, blaSHV, bla CTX-M and blaAmpC ). The phylogenetic relationship was determined by RAPD-PCR method. Among the drugs tested, the highest percentages of resistance were to amoxicillin (35.3%) and to sulfonamide (15.7%). Eleven antimicrobial resistance patterns were identified (A1 to A11), none of them presented a multiresistance profile (> 3 antimicrobials classes). There was 100% of positivity for the agfA gene, 92.2% for the lpfA gene, and no strain presented the sefA gene. Most of the isolates showed similarities in virulence potential, since they were simultaneously positive for two studied genes, agfA and lpfA (92.2%, 47/51). Of the 18 (35.3%) strains resistant to antimicrobials of the β-lactam class, 10 (55.5%) were positive to blaAmpC gene, five (27.8%) for blaCTX-M , two (11.1%) to blaSHV and no strain presented the blaTEM gene. The phylogenetic evaluation has shown the presence of five clusters (A, B, C, D and E) with similarity greater than 80%, and three distinct strains which were not grouped in any cluster. Cluster B grouped 33 strains, all positive for lpfA and agfA genes, from both, the broiler farming facility and the slaughterhouse, persistent throughout all the study period. This cluster also grouped 18 strains clones with genetic similarity greater than 99%, all isolated in the slaughterhouse. The presence of virulence genes associated with persistent strains clones for a long period, warns to the possibility of S. Infantis to form biofilm, and should be constantly monitored in broilers’ production chain, in order to know the profile of the strains that may contaminate the final product and evaluate the hazards that represents to public health.

show abstract

“…In the following steps such as handling, packaging, storage and transport of animal meal at room temperature, there were risks of contamination and recontamination by microorganisms that were distributed in the industry environment. It is important to consider that these studies were carried out in greases that used as raw material by-products generated from slaughtering under inspection of animal products (federal, state or municipal) (Larsen et al, 2014;Maciorowski et al, 2007;Melo et al, 2011;Moura et al, 2014;Pellegrini et al, 2015). In the European Union, Larsen et al (2014) reported a decrease in the presence of Salmonella from 0.7% to 0.5% in the diet and from 2.9% to 0.6% in meat and bone meal between 2002 and 2010.…”

Section: Resultsmentioning

confidence: 99%

Qualitative risk assessment of animal meal applied to swine production

Pellegrini

Celis

Costa

2017

International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

View full text Add to dashboard Cite

There are gaps in the pig production chain, particularly as regards the possible destinations of dead animals on farms. The production of animal meal presents itself as an alternative for the recycling of biological waste. The objective of this study was realize a qualitative microbiological risk assessment on animal meal in order to provide subsidies to indicate possible hazards and risks associated with the use of meal produced from dead pigs on farms. The microorganism Salmonella was the main hazard reported. The scenario tree presented 15 scenarios for contamination and recontamination of animal meal. For the first scenario was defined release and exposure risk levels as moderate, was obtained as the risk level of the occurrence moderate. The risk level of the consequence determined as low together with the level of occurrence obtained previously resulted in the final risk level low. For the second scenario defined release risk level as high and the exposure risk level as moderate, we obtained as moderate risk level of occurrence. The risk level of the consequence determined as high related to the level of occurrence previously obtained resulted in the final risk level high. From the information and scenarios considered, it was not possible to indicate the production of animal meal as a probable destination for dead animals on pig farms.

show abstract

Genes de virulência e diversidade genética em Salmonella spp. isoladas de amostras de origem suína

Cited by 8 publications

References 11 publications

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

Characteristics of virulence, resistance and genetic diversity of strains of Salmonella Infantis isolated from broiler chicken in Brazil

Qualitative risk assessment of animal meal applied to swine production

Contact Info

Product

Resources

About