Genes differentially expressed in broccoli as an early and late response to wounding stress

Torres-Contreras, Ana Mariel; Senés‐Guerrero, Carolina; Pacheco, Adriana; González-Agüero, Mauricio; Ramos-Parra, Perla A.; Cisneros‐Zevallos, Luis; Jacobo‐Velázquez, Daniel A.

doi:10.1016/j.postharvbio.2018.07.010

Cited by 42 publications

(30 citation statements)

References 40 publications

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“…Recently, it was reported that stress caused by wounding induces the accumulation of important bioactive compounds in Brassica plants, such as glucosinolates [29,30]. Many genes involved in both aliphatic and indolyl glucosinolate pathways were induced by wounding [30]. In the present study, all three transport-related genes were strongly induced by wounding stress.…”

Section: Discussionsupporting

confidence: 59%

“…To our knowledge, very few studies have reported on the expression levels of the GTR gene in response to various abiotic stresses under different treatments. Recently, it was reported that stress caused by wounding induces the accumulation of important bioactive compounds in Brassica plants, such as glucosinolates [29,30]. Many genes involved in both aliphatic and indolyl glucosinolate pathways were induced by wounding [30].…”

Section: Discussionmentioning

confidence: 99%

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Molecular Cloning and Characterization of Three Glucosinolate Transporter (GTR) Genes from Chinese Kale

Jiang

Liu

Cao

et al. 2019

Genes

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Chinese kale is a native vegetable in Southern China and the flowering stalk is the most commonly used edible part due to its high glucosinolate content and other nutritional qualities. The GTR protein played important roles in the glucosinolate transport process. In this study, three BocGTR1 genes were cloned from Chinese kale for the first time. Their gene structure, physicochemical properties, signal peptides, transmembrane structures, functional domains, second and third-order protein structures, and phylogenetic relationships were predicted. The expression levels of BocGTR1a and BocGTR1c were much higher than those of BocGTR1b in various tissues, especially in leaves and buds. In addition, the expression patterns of three genes were examined under various abiotic stresses or hormone treatment, including those induced by wounding, heat stress, methyl jasmonate, salicylic acid, salt, and MgCl2 treatment. BocGTR1a and BocGTR1c were strongly induced by wounding and heat stress. The expression of BocGTR1a and BocGTR1c was significantly silenced in plants transformed by RNAi technology. Total glucosinolate content was significantly decreased in mature leaves and increased in roots of RNAi-transformed plants compared to wild-type plants. In addition, we found that BocGTR1a and BocGTR1c may participate in glucosinolate accumulation in different tissues with a selection for specific glucosinolates. These results indicated that BocGTR1a and BocGTR1c may be the key genes involved in the glucosinolate accumulation in different tissues of Chinese kale.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Characterization of Three Glucosinolate Transporter (GTR) Genes from Chinese Kale

Jiang

Liu

Cao

et al. 2019

Genes

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“…These stresses negatively impact Rosa plant growth, flower quality and field performance (Xing et al ., ). Wounding stress induces the accumulation of important bioactive compounds in wounded tissues (Ioannidi et al ., ; Torres‐Contreras et al ., ). Understanding the responses to the wounding and oxidation of rose plants are of great importance to maintain the yield of these plants since the fresh flowers are frequently cut in R. rugosa gardens.…”

Section: Introductionmentioning

confidence: 97%

RrMYB5‐ and RrMYB10‐regulated flavonoid biosynthesis plays a pivotal role in feedback loop responding to wounding and oxidation in Rosa rugosa

Shen

Sun

Pan

et al. 2019

Plant Biotechnology Journal

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Summary Flavonoids play critical roles in plant responses to various stresses. Few studies have been reported on what the mechanism of activating flavonoid biosynthesis in plant responses to wounding and oxidation is. In this study, flavonoid metabolites and many MYB transcript factors from Rosa rugosa were verified to be induced by wounding and oxidation. RrMYB5 and RrMYB10, which belong to PA1‐ and TT2‐type MYB TFs, respectively, showed extremely high induction. Overexpression of RrMYB5 and RrMYB10 resulted in an increased accumulation of proanthocyanidins in R. rugosa and tobacco by promoting the expression of flavonoid structural genes. Transcriptomic analysis of the transgenic plants showed that most genes, involved in wounding and oxidation response and ABA signalling modulation, were up‐regulated by the overexpression of RrMYB10, which was very much similar to that observed in RrANR and RrDFR overexpression transgenics. RrMYB5 and RrMYB10 physically interacted and mutually activated each other's expressions. They solely or synergistically activated the different sets of flavonoid pathway genes in a bHLH TF EGL3‐independent manner. Eventually, the accumulation of proanthocyanidins enhanced plant tolerance to wounding and oxidative stresses. Therefore, RrMYB5 and RrMYB10 regulated flavonoid synthesis in feedback loop responding to wounding and oxidation in R. rugosa. Our study provides new insights into the regulatory mechanisms of flavonoid biosynthesis by MYB TFs and their essential physiological functions in plant responses to wounding and oxidative stresses.

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“…During fresh-cut processing, the tissue is inevitably subjected to wounding stress, which will induce defense responses to produce more secondary metabolites at the injured site or site adjacent to defend and heal the wounding damage [6]. It has been confirmed that wounding stress induces phenolic biosynthesis and enhances antioxidant capacity in various fruit and vegetables, such as carrot [7], potato [8], onion [9], pitaya [10], lettuce [11], celery [11], and sweet potato [11]. These findings indicate that wounding can be used as an effective and practical means to enhance the phenolic accumulation and improve the nutritional quality of the postharvest product.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the accumulation of phenols depends on the dynamic change between phenol synthesis and oxidation reaction. Furthermore, the level of phenols was regulated by many postharvest abiotic stresses, such as MeJA [8,14], ethylene, salicylic acid, systemin [15,16,17], UV radiation [18], citric acid [19], and light exposure [20] in wounded fruit and vegetables.…”

Section: Introductionmentioning

confidence: 99%

Effect of Methyl Jasmonate on Phenolic Accumulation in Wounded Broccoli

Guan

Jiang

et al. 2019

Molecules

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In order to find an efficient way for broccoli to increase the phenolic content, this study intended primarily to elucidate the effect of methyl jasmonate (MeJA) treatment on the phenolic accumulation in broccoli. The optimum concentration of MeJA was studied first, and 10 μM MeJA was chosen as the most effective concentration to improve the phenolic content in wounded broccoli. Furthermore, in order to elucidate the effect of methyl jasmonate (MeJA) treatment on phenolic biosynthesis in broccoli, the key enzyme activities of phenylpropanoid metabolism, the total phenolic content (TPC), individual phenolic compounds (PC), antioxidant activity (AOX) and antioxidant metabolism-associated enzyme activities were investigated. Results show that MeJA treatment stimulated phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), and 4-coumarin coenzyme A ligase (4CL) enzymes activities in phenylpropanoid metabolism, and inhibited the activity of polyphenol oxidase (PPO), and further accelerated the accumulation of the wound-induced rutin, caffeic acid, and cinnamic acid accumulation, which contributed to the result of the total phenolic content increasing by 34.8% and ferric reducing antioxidant power increasing by 154.9% in broccoli. These results demonstrate that MeJA in combination with wounding stress can induce phenylpropanoid metabolism for the wound-induced phenolic accumulation in broccoli.

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Genes differentially expressed in broccoli as an early and late response to wounding stress

Cited by 42 publications

References 40 publications

Molecular Cloning and Characterization of Three Glucosinolate Transporter (GTR) Genes from Chinese Kale

Molecular Cloning and Characterization of Three Glucosinolate Transporter (GTR) Genes from Chinese Kale

RrMYB5‐ and RrMYB10‐regulated flavonoid biosynthesis plays a pivotal role in feedback loop responding to wounding and oxidation in Rosa rugosa

Effect of Methyl Jasmonate on Phenolic Accumulation in Wounded Broccoli

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