Genes Encoding Calmodulin-binding Proteins in the Arabidopsis Genome

Reddy, Vaka S.; Ali, Gul Shad; Reddy, Anireddy S. N.

doi:10.1074/jbc.m111626200

Cited by 211 publications

(162 citation statements)

References 126 publications

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“…13,14,23 In this study, we screened an Arabidopsis cDNA expression library constructed from heat-treated plant seedlings using AtCaM2::HRP as a probe. 20,22 In all, 11 positive clones were obtained from a total of 5 Â 10 5 recombinant phages.…”

Section: Resultsmentioning

confidence: 99%

“…11,12 Since CaM is known to transduce Ca 2 þ -signals by modulating the activity of numerous and diverse CaMBPs, thereby generating physiological responses to various stimuli, many CaM and CaMBPs have been isolated with a view to furthering our understanding of Ca 2 þ -mediated plant signaling pathways. [13][14][15] However, many aspects of plant CaM and CaMBPs signaling, as well as their functional significance, remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants

et al. 2005

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Calmodulin (CaM) influences many cellular processes by interacting with various proteins. Here, we isolated AtBAG6, an Arabidopsis CaM-binding protein that contains a central BCL-2-associated athanogene (BAG) domain. In yeast and plants, overexpression of AtBAG6 induced cell death phenotypes consistent with programmed cell death (PCD). Recombinant AtBAG6 had higher affinity for CaM in the absence of free Ca 2 þ than in its presence. An IQ motif (IQXXXRGXXXR, where X denotes any amino-acid) was required for Ca 2 þ -independent CaM complex formation and single amino-acid changes within this motif abrogated both AtBAG6-activated CaM-binding and cell death in yeast and plants. A 134-amino-acid stretch, encompassing both the IQ motif and BAG domain, was sufficient to induce cell death. Agents generating oxygen radicals, which are known to be involved in plant PCD, specifically induced the AtBAG6 transcript. Collectively, these results suggest that AtBAG6 is a stress-upregulated CaM-binding protein involved in plant PCD.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants

et al. 2005

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“…Ca 2+ -CaM plays an essential role in the interpretation of Ca 2+ signatures and their transduction to the downstream signaling components by binding to and altering the activities of various enzymes as well as cytoskeletal and structural proteins (Reddy et al, 2002;Rato et al, 2004). In gymnosperm species, the initiation of germination and the maintenance of subsequent tube elongation depended on continuous protein synthesis (Fernando et al, 2001;Hao et al, 2005), in contrast to the angiosperm pollen tube growth, in which pollen grains have presynthesized a complement of proteins (Mascarenhas, 1993;Dai et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Combined Proteomic and Cytological Analysis of Ca2+-Calmodulin Regulation inPicea meyeriPollen Tube Growth

Chen

et al. 2008

Plant Physiology

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Ca 2+ -calmodulin (Ca 2+ -CaM) is a critical molecule that mediates cellular functions by interacting with various metabolic and signaling pathways. However, the protein expression patterns and accompanying serial cytological responses in Ca 2+ -CaM signaling deficiency remain enigmatic. Here, we provide a global analysis of the cytological responses and significant alterations in protein expression profiles after trifluoperazine treatment in Picea meyeri, which abrogates Ca 2+ -CaM signaling. Ninety-three differentially displayed proteins were identified by comparative proteomics at different development stages and were assigned to different functional categories closely related to tip growth machinery. The inhibition of Ca 2+ -CaM signaling rapidly induced an increase in extracellular Ca 2+ influx, resulting in dramatically increased cytosolic Ca 2+ concentrations and ultrastructural abnormalities in organelles as the primary responses. Secondary and tertiary alterations included actin filament depolymerization, disrupted patterns of endocytosis and exocytosis, and cell wall remodeling, ultimately resulting in perturbed pollen tube extension. In parallel with these cytological events, time-course experiments revealed that most differentially expressed proteins showed time-dependent quantitative changes (i.e. some signaling proteins and proteins involved in organelle functions and energy production changed first, followed by alterations in proteins related to cytoskeletal organization, secretory pathways, and polysaccharide synthesis). Taken together, Ca 2+ -CaM dysfunction induced serial cytological responses and temporal changes in protein expression profiles, indicating the pivotal role of Ca 2+ -CaM in the regulation of tip growth machinery.

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“…Three Arabidopsis genes (At5g57580, At4g31000 and At5g26920) displayed high similarity to Os12g36110. At5g57580 belongs to the Group II of CaMbinding proteins playing a role during the heat shockmediated elevation of cytosolic Ca 2+ (Reddy et al 2002). At4g31000 was shown to be BR-regulated.…”

Section: (D) (A) (B) (C) (E)mentioning

confidence: 99%

The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress

et al. 2015

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Genes Encoding Calmodulin-binding Proteins in the Arabidopsis Genome

Abstract: Analysis of the recently completed

Cited by 211 publications

References 126 publications

AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants

AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants

Combined Proteomic and Cytological Analysis of Ca2+-Calmodulin Regulation inPicea meyeriPollen Tube Growth

The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress

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