Genes Encoding Specific Nickel Transport Systems Flank the Chromosomal Urease Locus of Pathogenic Yersiniae

Sebbane, Florent; Mandrand‐Berthelot, Marie‐Andrée; Simonet, Michel

doi:10.1128/jb.184.20.5706-5713.2002

Cited by 50 publications

(42 citation statements)

References 40 publications

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“…The expression of the E. coli nik operon is negatively regulated at the transcriptional level by the NikR repressor protein when intracellular nickel levels are high (11). Similar Ni 2ϩ uptake systems have been identified in Brucella suis (18), Vibrio parahaemolyticus (23), Actinobacillus pleuropneumoniae (2), and Yersinia pseudotuberculosis (25), and in all cases this Ni 2ϩ -specific transporter is associated with ureolytic activity of the microorganisms.…”

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confidence: 78%

Identification and Characterization of the Nickel Uptake System for Urease Biogenesis in Streptococcus salivarius 57.I

Chen

Burne

2003

J Bacteriol

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Ureases are multisubunit enzymes requiring Ni 2؉ for activity. The low pH-inducible urease gene cluster in Streptococcus salivarius 57.I is organized as an operon, beginning with ureI, followed by ureABC (structural genes), and ureEFGD (accessory genes). Urease biogenesis also requires a high-affinity Ni 2؉ uptake system. By searching the partial genome sequence of a closely related organism, Streptococcus thermophilus LMG18311, three open reading frame (ORFs) homologous to those encoding proteins involved in cobalamin biosynthesis and cobalt transport (cbiMQO) were identified immediately 3 to the ure operon. To determine whether these genes were involved in urease biogenesis by catalyzing Ni 2؉ uptake in S. salivarius, regions 3 to ureD were amplified by PCRs from S. salivarius by using primers identical to the S. thermophilus sequences. Sequence analysis of the products revealed three ORFs. Reverse transcriptase PCR was used to demonstrate that the ORFs are transcribed as part of the ure operon. Insertional inactivation of ORF1 with a polar kanamycin marker completely abolished urease activity and the ability to accumulate 63 Ni 2؉ during growth. Supplementation of the growth medium with NiCl 2 at concentrations as low as 2.5 M partially restored urease activity in the mutant. Both wild-type and mutant strains showed enhanced urease activity when exogenous Ni 2؉ was provided at neutral pH. Enhancement of urease activity by adding nickel was regulated at the posttranslational level. Thus, ORF1, ORF2, and ORF3 are part of the ure operon, and these genes, designated ureM, ureQ, and ureO, respectively, likely encode a Ni 2؉ -specific ATP-binding cassette transporter.

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confidence: 78%

Identification and Characterization of the Nickel Uptake System for Urease Biogenesis in Streptococcus salivarius 57.I

Chen

Burne

2003

J Bacteriol

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“…Though distantly related ABC transporter systems from pathogenic Yersinia pseudotuberculosis and Brucella suis are also implicated in the high-affinity nickel uptake (33,49), many other representatives of this ABC transporter family are involved in uptake of other compounds, i.e., dipeptides and oligopeptides (1,37).…”

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confidence: 99%

Comparative and Functional Genomic Analysis of Prokaryotic Nickel and Cobalt Uptake Transporters: Evidence for a Novel Group of ATP-Binding Cassette Transporters

et al. 2006

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The transition metals nickel and cobalt, essential components of many enzymes, are taken up by specific transport systems of several different types. We integrated in silico and in vivo methods for the analysis of various protein families containing both nickel and cobalt transport systems in prokaryotes. For functional annotation of genes, we used two comparative genomic approaches: identification of regulatory signals and analysis of the genomic positions of genes encoding candidate nickel/cobalt transporters. The nickel-responsive repressor NikR regulates many nickel uptake systems, though the NikR-binding signal is divergent in various taxonomic groups of bacteria and archaea. B 12 riboswitches regulate most of the candidate cobalt transporters in bacteria. The nickel/cobalt transporter genes are often colocalized with genes for nickel-dependent or coenzyme B 12 biosynthesis enzymes. Nickel/cobalt transporters of different families, including the previously known NiCoT, UreH, and HupE/UreJ families of secondary systems and the NikABCDE ABC-type transporters, showed a mosaic distribution in prokaryotic genomes. In silico analyses identified CbiMNQO and NikMNQO as the most widespread groups of microbial transporters for cobalt and nickel ions. These unusual uptake systems contain an ABC protein (CbiO or NikO) but lack an extracytoplasmic solute-binding protein. Experimental analysis confirmed metal transport activity for three members of this family and demonstrated significant activity for a basic module (CbiMN) of the Salmonella enterica serovar Typhimurium transporter.The transition metals nickel and cobalt are essential cofactors for a number of prokaryotic enzymes involved in a variety of metabolic processes (36,41). Among the known nickeldependent enzymes are urease (8), [NiFe] hydrogenase, carbon monoxide dehydrogenase (Ni-CODH) (35), acetyl-coenzyme A decarbonylase/synthase (21), superoxide dismutase SodN (22), methyl-coenzyme M reductase (20), and glyoxylase I (50). In contrast to the diverse roles of nickel in microbial metabolism, cobalt is mainly found in the corrin ring of coenzyme B 12 , a cofactor involved in methyl group transfer and in rearrangement reactions (36). Since in natural environments, soluble Ni 2ϩ and Co 2ϩ are usually present only in trace amounts, the synthesis of the respective metalloenzymes requires high-affinity uptake of metal ions. Until recently, two major types of microbial high-affinity nickel and cobalt transporters were known: ATP-binding cassette (ABC) systems and secondary permeases of the NiCoT family (reviewed in reference 24).The NikABCDE system of Escherichia coli belongs to the nickel/peptide/opine ABC transporter family and is composed of the periplasmic binding protein NikA, two integral membrane components (NikB and -C), and two ATPases (NikD and -E) (43). The molecular basis of selective high-affinity binding of Ni 2ϩ remains elusive, although crystal structures of E. coli NikA have been determined by two approaches (10, 31).The two studies uncovered that N...

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“…sJX204 covers a portion of the C5080 (yddR) gene, which has homology to Yersinia genes involved in nickel transport into bacteria. Interestingly, in pathogenic Yersinia species these uptake genes are adjacent to the urease gene cluster, and mutants defective in nickel uptake cannot make functional urease (31). sJX198 overlaps C5433, which has homology to C 4 -dicarboxylate transport substrate binding genes, and C5434 encodes a hypothetical protein with no known homologies.…”

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confidence: 99%

Molecular Epidemiologic Identification of Escherichia coli Genes That Are Potentially Involved in Movement of the Organism from the Intestinal Tract to the Vagina and Bladder

et al. 2006

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A first step in urinary tract infection (UTI) pathogenesis in the otherwise healthy host is the movement of uropathogenic Escherichia coli from the intestinal tract to the urinary tract. We conducted a genomic subtraction to isolate genetic regions associated with this movement. A representative UTI isolate present in the rectum, vagina, and bladder of a woman with UTI was chosen as the tester; the driver was a phylogenetically distant rectal isolate (based on pulsed-field gel electrophoresis analysis) with a profile of uropathogenic virulence genes similar to that of the tester. Tester-specific regions identified by the subtraction were screened, using DNA dot blot hybridization, against a collection of 88 uropathogens isolated from the rectum, urine, and/or vagina of women with UTIs and 54 E. coli isolates from the same women that were found only in the rectum. Twelve genetic regions occurred more often in multisite isolates than in rectal site-only isolates. Eleven of these 12 genetic regions are homologous to regions in the sequenced uropathogenic E. coli CFT073 strain.

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Genes Encoding Specific Nickel Transport Systems Flank the Chromosomal Urease Locus of Pathogenic Yersiniae

Cited by 50 publications

References 40 publications

Identification and Characterization of the Nickel Uptake System for Urease Biogenesis in Streptococcus salivarius 57.I

Identification and Characterization of the Nickel Uptake System for Urease Biogenesis in Streptococcus salivarius 57.I

Comparative and Functional Genomic Analysis of Prokaryotic Nickel and Cobalt Uptake Transporters: Evidence for a Novel Group of ATP-Binding Cassette Transporters

Molecular Epidemiologic Identification of Escherichia coli Genes That Are Potentially Involved in Movement of the Organism from the Intestinal Tract to the Vagina and Bladder

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