Genes for human general transcription initiation factors TFIIIB, TFIIIB-associated proteins, TFIIIC2 and PTF/SNAPC: functional and positional candidates for tumour predisposition or inherited genetic diseases?

Purrello, Michele; Pietro, Cinzia Di; Rapisarda, A.; Amico, V.; Giunta, Veronica; Engel, Hartmut; Stevens, Sean; Hsieh, Yng-Ju; Teichman, M. A.; Wang, Zhengxin; Sichel, G.; Roeder, Robert G.; Grzeschik, Karl‐Heinz

doi:10.1038/sj.onc.1204604

Cited by 7 publications

(6 citation statements)

References 26 publications

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“…The data presented in this paper experimentally confirm our hypothesis that at least some GTA proteins may also be physiologically involved in the control of cell proliferation, at the same time underscoring the importance of natural selection within complex biopathological processes [ 22 , 47 ]. They also suggest possible ways to exploit molecular omic profiling to determine biological functions and design rational anticancer therapies.…”

Section: Discussionsupporting

confidence: 79%

Involvement of GTA protein NC2β in Neuroblastoma pathogenesis suggests that it physiologically participates in the regulation of cell proliferation

et al. 2008

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Background: The General Transcription Apparatus (GTA) comprises more than one hundred proteins, including RNA Polymerases, GTFs, TAFs, Mediator, and cofactors such as heterodimeric NC2. This complexity contrasts with the simple mechanical role that these proteins are believed to perform and suggests a still uncharacterized participation to important biological functions, such as the control of cell proliferation.

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Section: Discussionsupporting

confidence: 79%

Involvement of GTA protein NC2β in Neuroblastoma pathogenesis suggests that it physiologically participates in the regulation of cell proliferation

et al. 2008

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“…The GTF family of genes have roles as final targets of signal transduction pathways that control cell proliferation and differentiation. It is suggested that they have the ability to oncogenes an oncogene [34].…”

Section: Discussionmentioning

confidence: 99%

“…These genes are largely involved in suppression of cellular proliferation consistent with the putative cancer preventative properties of phytoestrogens. In particular, the genes TUBB , eIF‐5A , and GTF2F1 , are thought to play important roles in tumor development and are described as potential targets for chemotherapeutic agents [29,30,34]. These genes have not previously been reported to be regulated by genistein and warrant further investigation.…”

Section: Discussionmentioning

confidence: 99%

Changes in gene expressions elicited by physiological concentrations of genistein on human endometrial cancer cells

Konstantakopoulos

Montgomery

Chamberlain

et al. 2006

Molecular Carcinogenesis

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The incidence of hormone-related diseases such as prostatic, breast, ovarian, and endometrial cancer is lower in Asian populations compared to Western countries. High consumption of soybean products that are rich in phytoestrogens, predominantly genistein, is postulated to be responsible for the lower incidence of hormone-related disease, although the mechanism through which this effect might be mediated is unclear. In this study, microarray analysis was used to identify the changes in gene expression elicited by treatment of the human endometrial cancer cell line, Ishikawa, with genistein at both physiologically achievable and supraphysiological concentrations. Genistein treatment at 5 microM concentration induced multiple changes in gene expression including some implicated in oncogenesis. In contrast, treatment with a supraphysiological concentration of genistein predominantly activated stress response genes and showed very limited overlap with the genes regulated at lower concentrations. Of the genes regulated by genistein, 9.3% were also regulated by 17beta-estradiol suggesting that genistein exerts its response via the estrogen pathway. These results indicate that at physiological concentrations, genistein is able to elicit pleiotropic effects on a variety of pathways believed to be involved in tumorigenesis. Supraphysiological concentrations of genistein, such as those used in many previous studies, elicit changes in gene expression that are unlikely to occur in vivo.

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“…A similar statement may be made for a single apparatus. Following this assumption, our group began to characterize the genomics and transcriptomics of the GTA before the beginning of HGP (Purrello et al, 1994a(Purrello et al, , 1994b(Purrello et al, , 1995(Purrello et al, , 1996(Purrello et al, , 1998(Purrello et al, , 2001aDi Pietro et al, 1999. Our starting hypothesis was that the molecular complexity of this machinery was far too great for it to perform just a mechanical role and that some of these proteins could also be involved in regulatory functions, such as the control of cell proliferation and differentiation.…”

Section: The Tbp Family In Vertebratesmentioning

confidence: 98%

Genomics, Evolution, and Expression of TBPL2, a Member of the TBP Family

Pietro

Ragusa

Duro

et al. 2007

DNA and Cell Biology

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TBPL2 is the most recently discovered and less characterized member of the TATA box binding protein (TBP) family that also comprises TBP, TATA box binding protein-like 1 (TBPL1), and Drosophila melanogaster TBP related factor (TRF). In this paper we report our in silico and in vitro data on (i) the genomics of the TBPL2 gene in Homo sapiens, Pan troglodytes, Mus musculus, Rattus norvegicus, Gallus gallus, Xenopus tropicalis, and Takifugu rubripes; (ii) its evolution and phylogenetic relationship with TBP, TBPL1, and TRF; (iii) the structure of the TBPL2 proteins that belong to the recently identified group of the intrinsically unstructured proteins (IUPs); and (iv) TBPL2 expression in different organs and cell types of Homo sapiens and Rattus norvegicus. Similar to TBP, both the TBPL2 gene and protein are bimodular. The 3' region of the gene encoding the DNA binding domain (DBD) was well conserved during evolution. Its high homology to vertebrate TBP suggests that TBPL2 also should bind to the TATA box and interact with the proteins binding to TBP carboxy-terminal domain, such as the TBP associated factors (TAFs). As already demonstrated for TBP, TBPL2 amino-terminal segment is intrinsically unstructured and, even though variable among vertebrates, comprises a highly conserved motif not found in any other known protein. Absence of TBPL2 from the genome of invertebrates and plants demonstrates its specific origin within the subphylum of vertebrates. Our RT-PCR analysis of human and rat RNA shows that, similar to TBP, TBPL2 is ubiquitously synthesized even though at variable levels that are at least two orders of magnitude lower. Higher expression of TBPL2 in the gonads than in other organs suggests that it could perform important functions in gametogenesis. Our genomic and expression data should contribute to clarify why TBP has a general master role within the transcription apparatus (TA), whereas both TBPL1 and TBPL2 perform tissue-specific functions.

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Genes for human general transcription initiation factors TFIIIB, TFIIIB-associated proteins, TFIIIC2 and PTF/SNAPC: functional and positional candidates for tumour predisposition or inherited genetic diseases?

Cited by 7 publications

References 26 publications

Involvement of GTA protein NC2β in Neuroblastoma pathogenesis suggests that it physiologically participates in the regulation of cell proliferation

Involvement of GTA protein NC2β in Neuroblastoma pathogenesis suggests that it physiologically participates in the regulation of cell proliferation

Changes in gene expressions elicited by physiological concentrations of genistein on human endometrial cancer cells

Genomics, Evolution, and Expression of TBPL2, a Member of the TBP Family

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