Genes for ribosomal proteins S3, L16, L5 and S14 are clustered in the mitochondrial genome of Brassica napus L.

Ye, Fei; Bernhardt, J.; Abel, W. O.

doi:10.1007/bf00336784

Cited by 28 publications

(21 citation statements)

References 41 publications

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“…1A). Comparison of the amino acid sequences deduced from the cDNAs isolated in this study with the rps14 genes from other plant mitochondria (3,(17)(18)(19) as well as with the rice mitochondrial pseudogene showed 62-73% amino acid identity (Fig. 1B).…”

Section: Rps14-related Sequence In Rice Mitochondrial Genome Ismentioning

confidence: 69%

“…The presence of rps14 gene in the mitochondrial genome has been reported from lower plants such as liverwort (3) and chlorophyte alga (16). Among the higher plants, intact rps14 genes have been found in the mitochondrial genome of broadbean (17), Oenothera (18), and rapeseed (19), whereas rps14 is present as a pseudogene in Arabidopsis (20,21) and potato mitochondria (22). The nucleotide and deduced amino acid sequence comparison with those from other plant species showed that the rice rpl5 gene retains an intact ORF, whereas the original reading frame of the rps14 is interrupted by nucleotide deletions at four positions.…”

Section: Rps14-related Sequence In Rice Mitochondrial Genome Ismentioning

confidence: 99%

“…D86574). The N-terminal Deduced amino acid sequence of rice nuclear rps14 gene (this study) is aligned with amino acid sequences of mitochondrial rps14 genes from rice (this study), broadbean (17), Oenothera (18), rapeseed (19), and liverwort (3). Amino acid residues identical to rice nuclear RPS14 are indicated by dots.…”

Section: Rps14-related Sequence In Rice Mitochondrial Genome Ismentioning

confidence: 99%

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A single nuclear transcript encoding mitochondrial RPS14 and SDHB of rice is processed by alternative splicing: Common use of the same mitochondrial targeting signal for different proteins

Kubo

Harada

Hirai

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

104

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The rice mitochondrial genome has a sequence homologous to the gene for ribosomal protein S14 (rps14), but the coding sequence is interrupted by internal stop codons. A functional rps14 gene was isolated from the rice nuclear genome, suggesting a gene-transfer event from the mitochondrion to the nucleus. The nuclear rps14 gene encodes a long N-terminal extension showing significant similarity to a part of mitochondrial succinate dehydrogenase subunit B (SDHB) protein from human and a malarial parasite (Plasmodium falciparum). Isolation of a functional rice sdhB cDNA and subsequent sequence comparison to the nuclear rps14 indicate that the 5 portions of the two cDNAs are identical. The sdhB genomic sequence shows that the SDHB-coding region is divided into two exons. Surprisingly, the RPS14-coding region is located between the two exons. DNA gel blot analysis indicates that both sdhB and rps14 are present at a single locus in the rice nucleus. These findings strongly suggest that the two gene transcripts result from a single mRNA precursor by alternative splicing. Protein blot analysis shows that the size of the mature RPS14 is 16.5 kDa, suggesting removal of the N-terminal 22.6-kDa peptide region. Considering that the rice mitochondrial genome lacks the sdhB gene but contains the rps14-related sequence, transfer of the sdhB gene seems to have occurred before the transfer of the rps14 gene. The migration of the mitochondrial rps14 sequence into the already existing sdhB gene could bestow the capacity for nuclear expression and mitochondrial targeting.

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Section: Rps14-related Sequence In Rice Mitochondrial Genome Ismentioning

confidence: 69%

Section: Rps14-related Sequence In Rice Mitochondrial Genome Ismentioning

confidence: 99%

Section: Rps14-related Sequence In Rice Mitochondrial Genome Ismentioning

confidence: 99%

See 1 more Smart Citation

A single nuclear transcript encoding mitochondrial RPS14 and SDHB of rice is processed by alternative splicing: Common use of the same mitochondrial targeting signal for different proteins

Kubo

Harada

Hirai

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

104

View full text Add to dashboard Cite

show abstract

“…B Extension products obtained with L5-18 were coelectrophoresed with sequencing fragments generated with the same primer. A thymidine in the antisense strand (corresponding to an adenine in the sense strand) is detected as 5¢ terminal nucleotide, and lies within a conserved nonanucleotide motif (boxed) Comparison of rpl5 promoter sequences from pea and Oenothera A database analysis of the pea rpl5 upstream region revealed no striking sequence similarity to sequences anking rpl5 genes in other plant species (Brandt et al 1993;Schuster 1993;Ye et al 1993;QuinÄ ones et al 1996). However, inspection of the respective sequences in Oenothera identi®ed a dierent nonanucleotide motif that is identical to the consensus.…”

Section: Resultsmentioning

confidence: 97%

Cotranscription of the rpl5-rps14-cob gene cluster in pea mitochondria

Hoffmann¹,

Dombrowski²,

Guha³

et al. 1999

Mol Gen Genet

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In pea mitochondria the rpl5, rpsl4 and cob ORFs are clustered in a unique genomic environment and are cotranscribed into a 4.7-kb primary transcript and several other polycistronic RNAs with sizes between 4.0 and 2.3 kb. All of the larger RNAs terminate at a common 3' end, 52 nucleotides downstream of the cob gene. Transcription is initiated at a promoter about 1.3 kb upstream of the rpl5 start codon. The promoter sequence 5'-AATAAGAGA-3' corresponds to the highly conserved 5'-CRTAAGAGA-3' motif often found in promoters in dicot plants. Functional analysis in a homologous in vitro transcription system showed the pea rpl5 promoter to be active, despite the presence of an altered base in first position of the promoter motif. In Oenothera, in contrast to pea, transcription of the rpl5 gene is driven by a promoter motif that conforms perfectly to the consensus sequence. Double inverted repeats located in the 3' flanking regions of the rpsl4 and cob ORFs in pea were investigated with respect to their possible role in defining transcript termini and their potential function in controlling exo- and endonucleolytic processing or transcript stabilization.

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“…The rpl5-rps14-cob and rrn5-rn18 operons are conserved in most plants (Aubert et al 1992;Ye et al 1993;Quiaeones et al 1996;Hoffmann et al 1999), including squash and watermelon, but are rearranged in Cucumis (Fig. 4B).…”

Section: Mitochondrial Genome Organization Within Cucumismentioning

confidence: 98%

Organization of repetitive DNAs and the genomic regions carrying ribosomal RNA, cob, and atp9 genes in the cucurbit mitochondrial genomes

2003

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Plants in the genus Cucumis (cucumber and melon) have the largest mitochondrial genomes known among all plants, due in part to the accumulation of repetitive DNAs of varying complexities. Recombination among these repetitive DNAs should produce highly rearranged mitochondrial genomes relative to the smaller mitochondrial genomes of related plants. We cloned and sequenced mitochondrial genomic regions near the rRNA, atp9 and cob genes from cucumber, melon, squash and watermelon (all members of the Cucurbitaceae family), and compared to the previously sequenced mitochondrial genomes of Arabidopsis thaliana and sugar beet to study the distribution and arrangement of coding and repetitive DNAs. Cucumber and melon had regions of concentrated repetitive DNAs spread throughout the sequenced regions; few repetitive DNAs were revealed in the mitochondrial genomes of A. thaliana, sugar beet, squash and watermelon. Recombination among these repetitive DNAs most likely produced unique arrangements of the rrn18 and rrn5 genes in the genus Cucumis. Cucumber mitochondrial DNA had more pockets of dispersed direct and inverted repeats than melon and the other plants, and we did not reveal repetitive sequences significantly contributing to mitochondrial genome expansion in both cucumber and melon.

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Genes for ribosomal proteins S3, L16, L5 and S14 are clustered in the mitochondrial genome of Brassica napus L.

Cited by 28 publications

References 41 publications

A single nuclear transcript encoding mitochondrial RPS14 and SDHB of rice is processed by alternative splicing: Common use of the same mitochondrial targeting signal for different proteins

A single nuclear transcript encoding mitochondrial RPS14 and SDHB of rice is processed by alternative splicing: Common use of the same mitochondrial targeting signal for different proteins

Cotranscription of the rpl5-rps14-cob gene cluster in pea mitochondria

Organization of repetitive DNAs and the genomic regions carrying ribosomal RNA, cob, and atp9 genes in the cucurbit mitochondrial genomes

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