Genes in mice that affect susceptibility to cortisone-induced cleft palate are closely linked to Ir genes on chromosomes 2 and 17.

Abstract

Inbred and congeneic strains of mice have been examined for susceptibility to cortisone-induced cleft palate, and

A lymphocyte antigen encoded by a locus closely linked to H-3 on the second chromosome of the mouse.

“…It is known that genes linked to both H-2 and H-3 are involved in controlling the immune responses to Ea-1 (4,8) and H-Y (7, 9, 10) 0L% antigens. Genes linked to both H-2 and H-3 are involved also in controlling the susceptibility of mice to cortisone-induced cleft palate (24,25). New information about the genetic structure of the H-3 region should be useful in elucidating the biological mechanisms involved in these phenomena.…”

P1 and cosmid clones define the organization of 280 kb of the mouse H-2 complex containing the Cps-1 and Hsp70 loci

“…We have also observed evidence for dietary influences on susceptibility to cortisone-induced cleft palate (Gasser et al 1981), and, since the report of Tyan (1987), we have routinely used mouse chow with a high content of vitamin A (Purina Mouse Chow #5015, containing 30 IU/gram). Our report of a difference between B10.A(1R) and B 10.A(2R) (Gasser et al 1988) was based upon consumption of a high vitamin A diet, to which B 10.A(2R) is responsive while B 10.A(1R) is not.…”

Reply

“…Studies of congenic strains are appealing because they allow one to isolate individual genes to very small regions. If the data obtained in such experiments are not satisfying, it is always tempting to propose that different H-2-1inked genes are interacting with one another, and there was a time when we ourselves favored such models (Gasser et al 1981). However, in the light of our recent experiences, our preference is now to determine first whether all of the genes being proposed are indeed H-2-1inked.…”

“…This was not the case with certain other haplotypes, such as those present in B10.BAR5, B10.A(18R), and B10.A(2R), and in those cases H-2-1inkage of susceptibility or resistance could indeed be demonstrated (Gasser et al 1988;1994). B10.A(4R) and B10.A(5R) both have fairly low levels of susceptibility to cortisone-induced cleft palate when maintained on diets with either low or high vitamin A content (Gasser et al 1981). These two strains appear to carry recombinant haplotypes that are reciprocal crossovers, with the point of crossing over in each case being located within the second intron of the EB gene (Kabori et al 1986;Zinunerer and Passmore 1991).…”