Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant.

Aida, Mitsuhiro; Ishida, Tetsuya; Fukaki, Hidehiro; Fujisawa, Hisao; Tasaka, Masao

doi:10.1105/tpc.9.6.841

Cited by 1,286 publications

(1,163 citation statements)

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“…Protein of this family contains a highly conserved N-terminal DNA-binding domain and a diversified C-terminal domain. NAC was derived from the names of the first three described proteins containing the DNA-binding domain, namely NAM (no apical meristem), ATAF1-2 and CUC2 (cup-shaped cotyledon) (Souer et al 1996;Aida et al 1997). NAC proteins appear to be widespread in plants but no homolog has been identified thus far in other eukaryotes (Riechmann et al 2000).…”

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“…The early reported NAC genes have been implicated in various aspects of plant development. For examples, NAM from petunia (Souer et al 1996) and CUC1-2 (Aida et al 1997) from Arabidopsis are expressed in the primordial and meristem boundaries and playing important roles in controlling the formation of boundary cell and their mutants failed to develop shoot apical meristem. NAP (Sablowski and Meyerowitz 1998) from Arabidopsis acts as a target gene of AP3/PI to function in the transition between growth by cell division and cell expansion in stamens and petals.…”

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Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

et al. 2008

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Plants respond to adverse environment by initiating a series of signaling processes including activation of transcription factors that can regulate expression of arrays of genes for stress response and adaptation. NAC (NAM, ATAF, and CUC) is a plant specific transcription factor family with diverse roles in development and stress regulation. In this report, a stress-responsive NAC gene (SNAC2) isolated from upland rice IRA109 (Oryza sativa L. ssp japonica) was characterized for its role in stress tolerance. SNAC2 was proven to have transactivation and DNA-binding activities in yeast and the SNAC2-GFP fusion protein was localized in the rice nuclei. Northern blot and SNAC2 promoter activity analyses suggest that SNAC2 gene was induced by drought, salinity, cold, wounding, and abscisic acid (ABA) treatment. The SNAC2 gene was over-expressed in japonica rice Zhonghua 11 to test the effect on improving stress tolerance. More than 50% of the transgenic plants remained vigorous when all WT plants died after severe cold stress (4-8°C for 5 days). The transgenic plants had higher cell membrane stability than wild type during the cold stress. The transgenic rice had significantly higher germination and growth rate than WT under high salinity conditions. Over-expression of SNAC2 can also improve the tolerance to PEG treatment. In addition, the SNAC2-overexpressing plants showed significantly increased sensitivity to ABA. DNA chip profiling analysis of transgenic plants revealed many up-regulated genes related to stress response and adaptation such as peroxidase, ornithine aminotransferase, heavy metal-associated protein, sodium/hydrogen exchanger, heat shock protein, GDSL-like lipase, and phenylalanine ammonia lyase. Interestingly, none of the up-regulated genes in the SNAC2-overexpressing plants matched the genes up-regulated in the transgenic plants over-expressing other stress responsive NAC genes reported previously. These data suggest SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice.

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Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

et al. 2008

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“…NAC proteins commonly possess a conserved NAC domain at the N-terminus, which comprises nearly 160 amino acid residues that are divided into five subdomains (A-E) [6,9,10]. A sequence region of 60 residues within the NAC domain contains a unique TF fold consisting of a twisted-sheet bounded by a few helical elements [11].…”

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Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa

et al. 2010

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BackgroundNAC (NAM, ATAF1/2 and CUC2) domain proteins are plant-specific transcriptional factors known to play diverse roles in various plant developmental processes. NAC transcription factors comprise of a large gene family represented by more than 100 members in Arabidopsis, rice and soybean etc. Recently, a preliminary phylogenetic analysis was reported for NAC gene family from 11 plant species. However, no comprehensive study incorporating phylogeny, chromosomal location, gene structure, conserved motifs, and expression profiling analysis has been presented thus far for the model tree species Populus.ResultsIn the present study, a comprehensive analysis of NAC gene family in Populus was performed. A total of 163 full-length NAC genes were identified in Populus, and they were phylogeneticly clustered into 18 distinct subfamilies. The gene structure and motif compositions were considerably conserved among the subfamilies. The distributions of 120 Populus NAC genes were non-random across the 19 linkage groups (LGs), and 87 genes (73%) were preferentially retained duplicates that located in both duplicated regions. The majority of NACs showed specific temporal and spatial expression patterns based on EST frequency and microarray data analyses. However, the expression patterns of a majority of duplicate genes were partially redundant, suggesting the occurrence of subfunctionalization during subsequent evolutionary process. Furthermore, quantitative real-time RT-PCR (RT-qPCR) was performed to confirm the tissue-specific expression patterns of 25 NAC genes.ConclusionBased on the genomic organizations, we can conclude that segmental duplications contribute significantly to the expansion of Populus NAC gene family. The comprehensive expression profiles analysis provides first insights into the functional divergence among members in NAC gene family. In addition, the high divergence rate of expression patterns after segmental duplications indicates that NAC genes in Populus are likewise to have been retained by substantial subfunctionalization. Taken together, our results presented here would be helpful in laying the foundation for functional characterization of NAC gene family and further gaining an understanding of the structure-function relationship between these family members.

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“…Among the three members, after which this family is named, NAM (No Apical Meristem) from petunia is important for shoot apical meristem formation (Souer et al, 1996), CUC2 (cup-shaped cotyledon) is involved in controlling the formation of boundary cells of the meristem (Aida et al, 1997), and ATAF1, 2 show dual function in biotic and abiotic stress responses (Collinge and Boller, 2001;Delessert et al, 2005;Jensen et al, 2007;Lu et al, 2007;Jensen et al, 2008;Wang et al, 2009;Wu et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

A structural view of the conserved domain of rice stress-responsive NAC1

et al. 2011

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The importance of NAC (named as NAM, ATAF1, 2, and CUC2) proteins in plant development, transcription regulation and regulatory pathways involving proteinprotein interactions has been increasingly recognized. We report here the high resolution crystal structure of SNAC1 (stress-responsive NAC) NAC domain at 2.5 Å. Although the structure of the SNAC1 NAC domain shares a structural similarity with the reported structure of the ANAC NAC1 domain, some key features, especially relating to two loop regions which potentially take the responsibility for DNA-binding, distinguish the SNAC1 NAC domain from other reported NAC structures. Moreover, the dimerization of the SNAC1 NAC domain is demonstrated by both soluble and crystalline conditions, suggesting this dimeric state should be conserved in this type of NAC family. Additionally, we discuss the possible NAC-DNA binding model according to the structure and reported biological evidences.

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Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant.

Cited by 1,286 publications

References 22 publications

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa

A structural view of the conserved domain of rice stress-responsive NAC1

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