The crop types and agricultural characteristic are reconstructed using the archaeobiological proxies of pollen, seed and phytolith at Xishanping site in Gansu Province between 5250 and 4300 cal a BP. The agricultural activity strengthened in Xishanping from 5100 cal a BP. It appeared the earliest cultivation of prehistoric rice in the most northwest China at 5070 cal a BP. The sudden disappearance of conifers and expansion of chestnut trees is likely to be the result of selective hewing of conifers and cultivation of chestnuts at about 4600 cal a BP. There existed 8 crop types of foxtail millet, broomcorn millet, rice, wheat, barley, oats, soybean and buckwheat at Xishanping between 4650 and 4300 cal a BP, which cover the main crop types of the two origin centers of East and West Asia. Not only has the wheat and barley been approved to spread to northwestern China, but the earliest complexity agriculture in Neolithic China appeared in Tianshui, Gansu Province. archaeobiological proxy, broadening agriculture, Xishanping site, Gansu Province, 4650 cal a BP The Anthropocene as an individual geological episode containing special signification and content was suggested a new geological era [1,2] that is extracted from the Holocene of 10000 years [3] , in which humans have impact on the natural behavior of the Earth [1][2][3][4] . The further study on the Anthropocene will help us to discuss the characteristic of human activities especially the prehistoric agriculture that resulted in the changes of land, vegetation, and atmosphere and attend the naissance, conflicting, blending, and development of civilization [1,[4][5][6] .The climate fluctuation from the last deglaciation altered the survival state of humans in most regions in the world. The maladjustment between the population and natural resources stressed the prehistoric hunting-gathering humans to adopt the environmental change for survival using the new technology [7,8] . The agriculture, as one of the most important events appearing in the early Neolithic and developed rapidly [8][9][10][11][12][13] , is the most important economic activity in prehistoric society and the base of the forming and developing of civilization. Now, the biological evidence of the human agricultural activity became one of the significant studies of Anthropocene to reconstruct the agricultural history in different regions, and the economic movement and population growth and expansion were discussed. The records of archaeological proxy will help us to discuss the exchanging and blending of different cultures triggered by the agriculture expanding and spreading and to explore the contribution of agriculture activity to the naissance