Genes of Periodontopathogens Expressed During Human Disease

Song, Yohan; Kozarov, Emil; Walters, Sheila; Cao, Sam Linsen; Handfield, Martin; Hillman, Jeffrey D.; Progulske‐Fox, Ann

doi:10.1902/annals.2002.7.1.38

Cited by 20 publications

(26 citation statements)

References 11 publications

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“…Studies using in vivo induced antigen technology (IVIAT) [22] have the potential to identify large numbers of immunogenic antigens. The technique has been used to screen the entire A. actinomycetemcomitans genome and revealed 116 in vivo expressed antigens [23]. Further investigations using this method have shown that patients have a significantly greater serum antibody titer against six antigens than healthy controls, which supports the in vivo expression of these antigens, and even suggests their usefulness as disease markers [24].…”

Section: Introductionmentioning

confidence: 91%

“…Periodontal health was defined as no periodontal pockets exceeding 4 mm in a full dentition (28 teeth) and no attachment loss of more than 1 mm at more than one site, whereas gingival bleeding after probing indicative of gingival inflammation could occur. The mean age of the Moroccan AgP subjects was 18.5 years (range [14][15][16][17][18][19][20][21][22][23] and of the healthy control subjects 21.8 years (range 15-28). The mean age of the Danish AgP subjects was 36.8 (range 28-47) and of the healthy control subjects 30 years (27 and 33).…”

Section: Patient and Control Seramentioning

confidence: 99%

See 1 more Smart Citation

Proteomic and immunoproteomic analysis of Aggregatibacter actinomycetemcomitans JP2 clone strain HK1651

Rylev

Abduljabar

Reinholdt

et al. 2011

Journal of Proteomics

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Section: Introductionmentioning

confidence: 91%

Section: Patient and Control Seramentioning

confidence: 99%

Proteomic and immunoproteomic analysis of Aggregatibacter actinomycetemcomitans JP2 clone strain HK1651

Rylev

Abduljabar

Reinholdt

et al. 2011

Journal of Proteomics

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“…Moreover, many oral organisms including A. actinomycetemcomitans, P. gingivalis, F. nucleatum and S. gordonii have been shown to modulate expression of individual genes in epithelial cells (Belton et al 1999;Cao et al 2004;Darveau et al 1998;Fives-Taylor et al 1999;Guthmiller Lally and Korostoff 2001;Haraszthy et al 2000;Holt et al 1999;Korostoff et al 1998;Korostoff et al 2000;Lamont et al 1995;Lamont and Jenkinson 1998;Meyer Sreenivasan and Fives-Taylor 1991;Meyer Lippmann and Fives-Taylor 1996;Meyer Mintz and Fives-Taylor 1997;Nakhjiri et al 2001;Noguchi et al 2003;Shenker et al 1999;Shenker et al 2000;Shenker et al 2001;Song et al 2002;Yilmaz Watanabe and Lamont 2002;Yilmaz et al 2003;Zhang et al 2001a;Zhang Pelech and Uitto 2004;. Thus epithelial cells are capable of sensing and responding to oral bacteria at the transcriptional level.…”

Section: The Subgingival Microbial Challengementioning

confidence: 99%

Beyond Good and Evil in the Oral Cavity: Insights into Host-Microbe Relationships Derived from Transcriptional Profiling of Gingival Cells

2008

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In many instances, the encounter between host and microbial cells can be, through a longstanding evolutionary association, a balanced interaction whereby both cell types co-exist and inflict a minimal degree of harm on each other. In the oral cavity, despite the presence of large numbers of diverse organisms, health is the most frequent status. Disease will only ensue when the host-microbe balance is disrupted on a cellular and molecular level. With the advent of microarrays, it is now possible to monitor the responses of host cells to bacterial challenge in a global scale. However, microarray data are known to be inherently noisy, which is caused in part by their great sensitivity. Hence, we will address a number of important general considerations required to maximize the significance of microarray analysis in order to faithfully depict relevant host-microbe interactions. Several advantages and limitations of microarray analysis that may directly impact the significance of array data are highlighted and discussed. Further, this review revisits and contextualizes recent transcriptional profiles that were originally generated to specifically study intricate cellular interactions between gingival cells and four important plaque microorganisms. This is, to our knowledge, the first report that systematically investigates the cellular responses of a cell line to challenge by 4 different microorganisms. Of particular relevance to the oral cavity, the model bacteria span the entire spectrum of documented pathogenic potential from commensal to opportunistic to overtly pathogenic. These studies provide a molecular basis of the complex and dynamic interaction between the oral microflora and its host, which may lead, in the long run, to the development of novel, rational and practical therapeutic, prophylactic and diagnostic applications.

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“…Therefore, it is of importance to understand the mechanisms controlling the expression of these genes. In the case of Aa, Song et al [40] Cao et al [41], and Handfield et al [42] used an In Vivo Expression Technology (INVET) system to demonstrate the expression of 116 genes whose expression was induced following Aa infection of periodontitis patients, that were not expressed during in vitro growth of Aa on culture media. Some of these observations may well be explained by the findings of the present investigation.…”

Section: Resultsmentioning

confidence: 99%

Prophage induction in lysogenic Aggregatibacter actinomycetemcomitans cells co-cultured with human gingival fibroblasts, and its effect on leukotoxin release

Stevens

Santos

Zuanazzi

et al. 2013

Microbial Pathogenesis

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Lysogeny is common among strains of the periodontal pathogen Aggregatibacter actinomycetemcomitans. Since lysogenic induction is known to result in the increased synthesis and release of bacterial toxins from lysogens, it would be important to elucidate the conditions under which induction of these bacteria may occur. Co-cultures of A. actinomycetemcomitans strains (either lysogenic or non-lysogenic) and human cells (either gingival fibroblasts or pharyngeal epithelial cells) were prepared. Following incubation, bacteriophage titers of up to 6.2 × 10(7) pfu/ml were detected in the cell-free, spent culture media from the co-cultures of the lysogenic A. actinomycetemcomitans strains and the fibroblasts. Little (maximum of 2 × 10(0) pfu/ml) or no titers of phage could be detected in the mono-cultures of the lysogenic A. actinomycetemcomitans strains alone. In contrast, no phage were detectable in the cell-free spent culture media of the lysogens cocultured with the epithelial cells. Futhermore, co-culture of the A. actinomycetemcomitans lysogens with the fibroblasts resulted in enhanced release of the A. actinomycetemcomitans leukotoxin into the culture medium, in comparison with the spent culture media from mono-cultures of the lysogens alone. These results are consistent with the concept that interaction with fibroblasts may mediate prophage induction in lysogenic strains of A. actinomycetemcomitans, and that leukotoxin release is greatly augmented following induction of the lysogens.

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Genes of Periodontopathogens Expressed During Human Disease

Abstract: MAT has proven useful in identifying previously unknown in vivo-induced genes that are likely involved in virulence and are thus excellent candidates for use in diagnostic : and therapeutic strategies, including vaccine design.

Cited by 20 publications

References 11 publications

Proteomic and immunoproteomic analysis of Aggregatibacter actinomycetemcomitans JP2 clone strain HK1651

Proteomic and immunoproteomic analysis of Aggregatibacter actinomycetemcomitans JP2 clone strain HK1651

Beyond Good and Evil in the Oral Cavity: Insights into Host-Microbe Relationships Derived from Transcriptional Profiling of Gingival Cells

Prophage induction in lysogenic Aggregatibacter actinomycetemcomitans cells co-cultured with human gingival fibroblasts, and its effect on leukotoxin release

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