Genes related to high temperature tolerance during maize seed germination

Abstract

ABSTRACT. The identification of genes related to heat tolerance is fundamental for the development of high-quality seeds that are tolerant to heat stress condition. The objective of this study was to evaluate maize lineages and the gene expression involved in high temperature tolerance during germination using physiological tests, proteomics, and transcriptome analysis. Seeds from six maize lineages (30, 44, 54, 63, 64, and 91) with different levels of tolerance to high temperatures were used. Lineages 54 and 91 were observed to be more tolerant to high temperature conditions. The highest expression of α-amylase was observed in maize seeds from lineages 30 and 91 that were subjected to controlled deterioration. The highest expression of α-amylase was observed in maize seeds from lineages 30 and 91 that were subjected to controlled deterioration; with the controlled deterioration, the highest level of gene expression did not occur in the most tolerant materials; the association of lower expression of genes involved in heat-resistant protein systems was observed in seeds from lineage 44, which were more susceptible to high temperatures, and the highest gene expression of LEA D-34, ZmAN13, and AOX-1 was observed in seeds from lineage 64 when submitted to controlled deterioration.

Transcriptional Profiling and Identification of Heat-Responsive Genes in Perennial Ryegrass by RNA-Sequencing

“…A wide spectrums of heat response genes have been identified in different plant species using transcriptomic methods, including Arabidopsis (Rizhsky et al, 2002; Larkindale and Vierling, 2008; Song et al, 2016), rice ( Oryza sativa ) (Sarkar et al, 2014; Wu et al, 2015), maize ( Zea mays ) (Dutra et al, 2015; Frey et al, 2015; Casaretto et al, 2016), tomato ( Solanum lycopersicum ) (Bita et al, 2011; Cheng et al, 2012), potato ( Solanum tuberosum ) (Ginzberg et al, 2009; Tang et al, 2016), carnation ( Dianthus caryophyllus ) (Wan et al, 2015), wheat ( Triticum aestivum ) (Qin et al, 2008), and Brachypodium distachyon (Chen and Li, 2017). However, little information is available regarding heat responsive genes at global transcriptome level in the perennial cool-season grasses, especially perennial ryegrass.…”

Considerations of AOX Functionality Revealed by Critical Motifs and Unique Domains

“…One such monocot which needs more study is maize, the last of the quartet of monocot species responsible for a significant portion of food production [ 1 ]. While there has been some work done on AOX genes in maize [ 49 , 50 , 51 , 52 , 53 , 54 ], the new information discovered should hopefully spur a more extensive characterization of the gene and protein family to better understand its evolution and, hopefully, utilize this information in food production. Another monocot which needs study is Panicum virgatum , which, while not used for food, has immense potential in the field of sustainable bioenergy production, where AOX has also been shown to increase biomass [ 55 , 56 , 57 ].…”

Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings

“…In the 32-T, 91-T and 57-NT lines, higher expression of the CAT enzyme was observed in seeds. Catalase is an antioxidant enzyme that catalyzes the conversion of hydrogen peroxide (H2O2) to water (H2O), and its lower activity may be associated with a decrease in oxidative damage prevention mechanisms (Dutra et al 2015). On the other hand, it has been observed in other research, in different species, the increase of the expression of this enzyme under stress conditions (Deuner et al 2011).…”