Genes Required for GLP-1 Asymmetry in the EarlyCaenorhabditis elegansEmbryo

Crittenden, Sarah L.; Rudel, David; Binder, Joe; Evans, Thomas C.; Kimble, Judith

doi:10.1006/dbio.1996.8413

Cited by 30 publications

(9 citation statements)

References 15 publications

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“…This finding supports our model, because ectopic POS-1 in the anterior blastomeres would be expected to suppress translation. However, Crittenden et al (Crittenden et al, 1997) reported that in par-1 embryos, GLP-1 was detected in all blastomeres, in a manner similar to pos-1 embryos. We examined POS-1 expression in par-1(RNAi) embryos, and found that POS-1 was abundant in all blastomeres (data not shown).…”

Section: A Model For the Translational Regulation Of Maternal Glp-1 Mmentioning

confidence: 95%

Translational control of maternal glp-1 mRNA by POS-1 and its interacting protein SPN-4 in Caenorhabditis elegans

et al. 2003

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The translation of maternal glp-1 mRNAs is regulated temporally and spatially in C. elegans embryos. The 3′ UTR (untranslated region) of the maternal glp-1 mRNA is important for both kinds of regulation. The spatial control region is required to suppress translation in the posterior blastomeres. The temporal one is required to suppress translation in oocytes and one-cell stage embryos. We show that a CCCH zinc-finger protein, POS-1, represses glp-1 mRNA translation by binding to the spatial control region. We identified an RNP-type RNA-binding protein, SPN-4, as a POS-1-interacting protein. SPN-4 is presentdevelopmentally from the oocyte to the early embryo and its distribution overlaps with that of POS-1 in the cytoplasm and P granules of the posterior blastomeres. SPN-4 binds to a subregion of the temporal control region in the 3′ UTR and is required for the translation of glp-1 mRNA in the anterior blastomeres. We propose that the balance between POS-1 and SPN-4 controls the translation of maternal glp-1 mRNA.

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Section: A Model For the Translational Regulation Of Maternal Glp-1 Mmentioning

confidence: 95%

Translational control of maternal glp-1 mRNA by POS-1 and its interacting protein SPN-4 in Caenorhabditis elegans

et al. 2003

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“…Several genes required for different aspects of glp-1 regulation have been identified in mutant screens. Genes that control early cell polarity are necessary for GLP-1 localization in the embryo, presumably because they regulate the activities or localization of glp-1 regulators (Crittenden et al, 1997;Rose and Kemphues, 1998). In addition, two functionally redundant proteins, MEX-5 and MEX-6, act downstream of polarity genes and promote GLP-1 expression in anterior embryonic cells (Schubert et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Translational repression of aC. elegansNotch mRNA by the STAR/KH domain protein GLD-1

Marin¹,

2003

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“…The transition zone is characterized by the appearance of crescent-shaped nuclei indicating differentiation into leptotene/zygotene of meiotic prophase I. Under normal conditions a wild-type adult worm has approximately 22–23 germ cell diameters (CD) between the DTC and the appearance of a small population of leptotene/zygotene nuclei (Crittenden et al, 1997). We found that the proliferative mitotic zone decreased from approximately 22 cell diameters (CD) in the control worms down to 14–15 CDs in the 24-hour feeding experiment (Figure 6).…”

Section: Resultsmentioning

confidence: 99%

Stress response pathways protect germ cells from omega-6 polyunsaturated fatty acid-mediated toxicity in Caenorhabditis elegans

Webster¹,

Deline²,

Watts³

2013

Developmental Biology

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Polyunsaturated fatty acids serve both structural and functional roles as membrane components and precursors for a number of different factors involved in inflammation and signaling. These fatty acids are required in the human diet, although excess dietary intake of omega-6 fatty polyunsaturated fatty acids may have a negative influence on human health. In the model nematode, Caenorhabditis elegans, dietary exposure to dihomo- gamma-linolenic acid (DGLA), an omega-6 fatty acid, causes the destruction of germ cells and leads to sterility. In this study we used genetic and microscopic approaches to further characterize this phenomenon. We found that strains carrying mutations in genes involved in lipid homeostasis enhanced sterility phenotypes, while mutations reducing the activity of the conserved insulin/IGF signaling pathway suppressed sterility phenotypes. Exposure to a mild heat stress prior to omega-6 fatty acid treatment led to an adaptive or hormetic response, resulting in less sterility. Mutations in skn-1 and knockdown of genes encoding phase II detoxification enzymes led to increased sterility in the presence of dietary DGLA. Thus, detoxification systems and genetic changes that increase overall stress responses protect the germ cells from destruction. Microscopic analyses revealed that dietary DGLA leads to deterioration of germ cell membranes in the proliferative and transition zones of the developing germ line. Together, these data demonstrate that specific omega-6 polyunsaturated fatty acids, or molecules derived from them, are transported to the germ line where they disrupt the rapidly expanding germ cell membranes, leading to germ cell death.

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Genes Required for GLP-1 Asymmetry in the EarlyCaenorhabditis elegansEmbryo

Cited by 30 publications

References 15 publications

Translational control of maternal glp-1 mRNA by POS-1 and its interacting protein SPN-4 in Caenorhabditis elegans

Translational control of maternal glp-1 mRNA by POS-1 and its interacting protein SPN-4 in Caenorhabditis elegans

Translational repression of aC. elegansNotch mRNA by the STAR/KH domain protein GLD-1

Stress response pathways protect germ cells from omega-6 polyunsaturated fatty acid-mediated toxicity in Caenorhabditis elegans

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