GeneScan Reverse Transcription-PCR Assay for Detection of Six Common Respiratory Viruses in Young Children Hospitalized with Acute Respiratory Illness

Erdman, Dean D.; Weinberg, Geoffrey A.; Edwards, Kathryn M.; Walker, Frances J.; Anderson, Barbara; Winter, Jörn; González, Mónica Suárez; Anderson, Larry J.

doi:10.1128/jcm.41.9.4298-4303.2003

Cited by 115 publications

(102 citation statements)

References 33 publications

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“…Extracts were then tested by an RT-PCR assay panel for RSV, HPIV1, -2, and -3, and influenza A and B viruses using fluorescence-labeled primers for amplification and automated capillary electrophoresis and GeneScan s (Applied Biosystems, Foster City, CA, USA) fragment analysis to achieve sensitive and specific product detection. 6 For this study, the panel was expanded to include picornaviruses (inclusive of rhinoviruses and enteroviruses) using primers to conserved regions of the picornavirus 5…”

Section: Methodsmentioning

confidence: 99%

Active surveillance for respiratory virus infections in adults who have undergone bone marrow and peripheral blood stem cell transplantation

Roghmann

Ball

Erdman

et al. 2003

Bone Marrow Transplant

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Summary:Community-acquired respiratory virus (RV) infections are an important cause of disease in immunocompromised adults with cancer. To investigate the viral etiology, incidence, clinical presentation, and outcome of RV infections in an outpatient cohort of adult bone marrow or peripheral blood stem cell transplant (SCT) recipients, we monitored 62 outpatient volunteers from January 1 to April 30, 2001. A nasopharyngeal aspirate was collected from subjects when they reported new respiratory symptoms and tested for RV (influenza A, influenza B, human parainfluenza 1-3, adenovirus, and respiratory syncytial virus) by culture and reverse transcription polymerase chain reaction (RT-PCR) assay. Of 62 subjects enrolled, 27% had received allogeneic SCT and 45% were within 1 year of their transplant. In all, 35 participants (56%) reported 37 episodes of respiratory symptoms. Of the 37 specimens tested, five (14%) were positive for RV by culture and 20 (54%) were positive by RT-PCR. Only six patients with RV infections developed lower respiratory tract illnesses; these patients had received either autologous or allogeneic transplants and developed illnesses between 41 and 2666 days post transplant. Although RV infections were common in SCT outpatients during the RV season, most participants had upper respiratory tract infections, which resolved without the need for hospitalization.

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Section: Methodsmentioning

confidence: 99%

Active surveillance for respiratory virus infections in adults who have undergone bone marrow and peripheral blood stem cell transplantation

Roghmann

Ball

Erdman

et al. 2003

Bone Marrow Transplant

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“…The sensitivity of GeneScan RT-PCR for Influenza virus, RSV and hMPV is very high, described by Erdman et al as a sensitive and specific method for viral detection 20 . During 2002 and 2003, low activity of influenza virus in the elderly population in the community was described in the city of São Paulo, Brazil 21 .…”

Section: Phylogenetic Analysis Of Clinical Hrv Isolatesmentioning

confidence: 99%

Viral etiology among the elderly presenting acute respiratory infection during the influenza season

Watanabe

Carraro

Candeias

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: Acute respiratory tract infections are the most common illness in all individuals. Rhinoviruses have been reported as the etiology of more than 50% of respiratory tract infections worldwide. The study prospectively evaluated 47 elderly individuals from a group of 384 randomly assigned for acute respiratory viral infections (cold or flu) and assessed the occurrence of human rhinovirus (HRV), influenza A and B, respiratory syncytial virus and metapneumovirus (hMPV) in Botucatu, State of São Paulo, Brazil. Methods: Forty-nine nasal swabs collected from 47 elderly individuals following inclusion visits from 2002 to 2003 were tested by GenScan RT-PCR. HRV-positive samples were sequenced for phylogenetic analysis. Results: No sample was positive for influenza A/B or RSV. HRV was detected in 28.6% (14/47) and hMPV in 2% (1/47). Of 14 positive samples, 9 isolates were successfully sequenced, showing the follow group distribution: 6 group A, 1 group B and 2 group C HRVs. Conclusions: The high incidence of HRV during the months of the influenza season requires further study regarding HRV infection impact on respiratory complications among this population. Infection caused by HRV is very frequent and may contribute to increasing the already high demand for healthcare during the influenza season.

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“…5 After excluding influenza A infection (H1N1 2009 and seasonal influenza), the negative samples were tested for the presence of the following viruses as described previously: seasonal influenza virus B (IBV) with primers targeting the NS gene, 6 human rhinovirus (HRV) with primers targeting the 5'NCR and VP4/VP2 genes, 7 human metapneumovirus (hMPV) with primers targeting the fusion protein gene, 8 adenovirus (AdV) with primers targeting the hexon gene 9 and human respiratory syncytial virus (HRSV) with primers targeting the fusion protein gene. 10 The eluted RNA was transcribed into cDNA by moloney murine reverse transcriptase (MMLV-RT; Invitrogen, Carlsbad, CA) using random primers (Invitrogen, Carlsbad, CA) for 1 h at 37°C. The MMLV-RT was subsequently denatured at 70°C.…”

Section: Molecular Diagnosticsmentioning

confidence: 99%

Respiratory virus infections among hospitalized patients with suspected influenza A H1N1 2009 virus during the first pandemic wave in Brazil

Watanabe¹,

Carraro²,

Moreira³

et al. 2011

Braz J Infect Dis

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GeneScan Reverse Transcription-PCR Assay for Detection of Six Common Respiratory Viruses in Young Children Hospitalized with Acute Respiratory Illness

Cited by 115 publications

References 33 publications

Active surveillance for respiratory virus infections in adults who have undergone bone marrow and peripheral blood stem cell transplantation

Active surveillance for respiratory virus infections in adults who have undergone bone marrow and peripheral blood stem cell transplantation

Viral etiology among the elderly presenting acute respiratory infection during the influenza season

Respiratory virus infections among hospitalized patients with suspected influenza A H1N1 2009 virus during the first pandemic wave in Brazil

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