Genetic alterations in normal epithelium of colorectal cancer patients may be a useful indicator for subsequent metachronous tumor development

Abstract

For accurate LOH analysis, nonmalignant lymphocytes from blood should be used as the appropriate normal DNA sample. Focusing on the identification of high-risk patients for microsatellite alterations in histologically normal colorectal epithelium can be a useful indicator of subsequent metachronous tumor development after colorectal surgery. For accurate LOH analysis, nonmalignant lymphocytes from blood should be used as the appropriate normal DNA sample. Focusing on the identification of high-risk patients for microsatellite alterations in histologically normal colorectal epithelium can be a useful indicator of subsequent metachronous tumor development after colorectal surgery.

Simultaneous assessment of DNA ploidy and biomarker expression in paraffin‐embedded tissue sections

“…The combination of aneuploidy and normal‐appearing epithelium with proliferation confined to the basal cell layers shows that ploidy alterations may precede the development of dysplasia. This is in agreement with the observation that even alterations in multiple malignancy related genes do not necessarily result in recognizable cellular features 35 . By using fluorescence microscopy, simultaneous assessment of other relevant markers will increase the power of this analysis.…”

“…An example of the applicability of the technique described here is the study of so‐called fields as precursors of premalignancies 34 . The field concept has been extensively studied by molecular analysis of microdissected areas in tissue sections 35,36 . It was shown that normal‐appearing resection margins of squamous cell carcinoma often contain genetic and phenotypic alterations 36 .…”