Genetic alterations of HER genes in chromophobe renal cell carcinoma

Weng, Wen Hui; Chen, Ying Tzu; Yu, Kai; Chang, Yu‐Ling; Chuang, Cheng Keng; Pang, See Tong

doi:10.3892/ol.2016.4198

Cited by 10 publications

(9 citation statements)

References 27 publications

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“…In RCC, altered chemosensitivity mediated by 17-β-estradiol (E2) has been reported, so adjuvant therapy with E2 is possible as in other human diseases [ 24 , 45 ]. The molecular mechanisms of the action of E2 in RCC cells have been reported in recent years [ 8 , 10 , 13 , 43 , 45 ]. However, diverse genetic alterations are linked to renal cancer progression and to our ability to predict its risk [ 13 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Cellular effects induced by 17-β-estradiol to reduce the survival of renal cell carcinoma cells

Huang

et al. 2016

J Biomed Sci

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BackgroundRenal cell carcinoma (RCC) is an adult malignancy with 2:1 men-to-women ratio, which implies the possible role of sex hormones in RCC carcinogenesis. One of the predominant sex hormones in women before menopause, 17-β-estradiol (or E2), may regulate RCC growth by cellular mechanisms that are still not fully understood.MethodsThe expression levels of E2 receptors (ER1 and ER2) were determined in different RCC cell lines. The DNA damage response induced by E2 was determined by a DNA double-strand break marker γH2AX. To study the possible effect of E2 on oxidative stress response, RCC cells were stained with 2,7-dichlorofluorescein diacetate and analyzed by flow cytometry. Upregulation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) ser40 phosphorylation in response to oxidative stress was detected by immunoblotting. Finally, annexin V/propidium iodide (PI) double staining assay was used to determine E2-induced cellular apoptosis.ResultsVariable expression of ER1 and ER2 were found in the RCC cell lines studied (786-O, A498, and ACHN), in which ACHN and A498 showed highest and lowest ER expression, respectively. In A498 cells, E2 induced DNA double-strand breaks with positive staining of γH2AX. On the other hand, the level of reactive oxidative species were elevated in ACHN cells after E2 treatment. The E2-induced oxidative stress also induced the Ser40 phosphorylation and nuclear translocation of Nrf2. Finally, we also demonstrated that E2 induced apoptosis as revealed by annexin V/PI double staining.ConclusionsIn this study, we demonstrated the cellular effects of E2 on DNA repair, ROS production as well as Nrf2 activation, and apoptosis in RCC cell lines. Together these cellular alterations may contribute to the reduced viability of RCC cells following E2 treatment.

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Section: Introductionmentioning

confidence: 99%

Cellular effects induced by 17-β-estradiol to reduce the survival of renal cell carcinoma cells

Huang

et al. 2016

J Biomed Sci

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“…The erbB4 gene, also known as HER4 gene, is an oncogene that encodes the fourth human epidermal growth factor receptor (HER4). Studies show that it is overexpressed in renal carcinoma and is associated with lymph node metastasis, TNM staging and postoperative survival rate of renal carcinoma (Weng et al, 2016). However, no gene annotation related to HIF-1β was found in the GO database.…”

Section: Resultsmentioning

confidence: 99%

Involvement of erbB4 and tumor marker genes in renal carcinoma regulatory network

Xiao-fu

Hao²,

Xu³

et al. 2017

Saudi Journal of Biological Sciences

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BackgroundRenal carcinoma is a common urologic tumor, and there is no ideal tumor marker for clinical diagnosis except for imaging diagnosis. This study aims to screen the serum tumor markers closely related with the benign and malignant of renal carcinoma out and chart out the regulatory network that involves renal carcinoma-related genes.MethodsBased on 96 pathologically diagnosed renal cancer patients, factors strongly linked to renal carcinoma character were selected using Fisher discriminant analysis. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were utilized to manipulate function annotation of erbB4 and the selected genes and pathway analysis.ResultsFour essential tumor markers CYFRA21-1, CA125, VHL and HIF-1β were successfully screened out. Using GO and KEGG databases, the regulatory network of renal cancer cell escaping apoptosis was charted out on the basis of erbB4 signaling pathway.ConclusionSerum tumor marker genes play a certain part in the genesis and development of renal carcinoma. We preliminarily illustrated the molecular mechanism of these markers to predict tumor, laying a foundation for further exploration in renal carcinoma.

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“…chRCC is the third most common histological subtype of RCC, behind clear cell RCC and papillary RCC (3); it accounts for 5–7% of all RCC cases (4). Although patients with chRCC have a better prognosis compared with other subtypes, the long-term outcomes are highly variable and there is a 5–10% probability of eventually developing metastasis (7).…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that ~64,000 new cases were diagnosed in 2017 in the USA (1), and this value has risen by 2–4% each year (2). Chromophobe RCC (chRCC) is the third most common histological subtype of RCC (3), comprising 5–7% of all RCC cases (4). Due to advances in technology for the diagnosis and treatment of chRCC, the 5-year survival rate of chRCC is >75% (5) and the outcome is typically favorable when compared with that of other subtypes (6).…”

Section: Introductionmentioning

confidence: 99%

Identification of CFTR as a novel key gene in chromophobe renal cell carcinoma through bioinformatics analysis

Wang

Chai

2019

Oncol Lett

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Chromophobe renal cell carcinoma (chRCC), the third most common histological subtype of RCC, comprises 5–7% of all RCC cases. The aim of the present study was to identify potential biomarkers for chRCC and to examine the underlying mechanisms. A total of 4 profile datasets were downloaded from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of DEGs were performed with the Database for Annotation, Visualization and Integrated Discovery. A protein-protein interaction (PPI) network was constructed to predict hub genes. Hub gene expression within chRCC across multiple datasets, as well as overall survival, were investigated by utilizing the Oncomine platform and UALCAN dataset, separately. A total of 266 DEGs (88 upregulated genes and 168 downregulated genes) were identified from 4 profile datasets. Integrating the results from the PPI network, Oncomine platform and survival analysis, CFTR was screened as a key factor in the prognosis of chRCC. GO and KEGG analysis revealed that 266 DEGs were mainly enriched in 17 terms and 9 pathways. The present study identified key genes and potential molecular mechanisms underlying the development of chRCC, and CFTR may be a potential prognostic biomarker and novel therapeutic target for chRCC.

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Genetic alterations of HER genes in chromophobe renal cell carcinoma

Cited by 10 publications

References 27 publications

Cellular effects induced by 17-β-estradiol to reduce the survival of renal cell carcinoma cells

Cellular effects induced by 17-β-estradiol to reduce the survival of renal cell carcinoma cells

Involvement of erbB4 and tumor marker genes in renal carcinoma regulatory network

Identification of CFTR as a novel key gene in chromophobe renal cell carcinoma through bioinformatics analysis

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