Genetic Analyses Confirm SNPs in HSPA8 and ERBB2 are Associated with Milk Protein Concentration in Chinese Holstein Cattle

Liu, Cong; Wang, Miao; Cai, Wentao; Liu, Shuli; Zhou, Chixing; Yin, Hongwei; Sun, Dongxiao; Zhang, Shengli

doi:10.3390/genes10020104

Cited by 5 publications

(4 citation statements)

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“…They cause shear abnormalities by altering the structure of the shear site, which may lead to changes in protein structure and function [44]. In addition, several SNPs sites in the intron region of the erb-b2 receptor tyrosine kinase 2 (ERBB2) gene were reported to be significantly associated with milk protein content in Chinese Holstein cows [45]. Five SNPs (g.29029T>C, g.29050A>G, g.29245C>T, g.29305C>T and g.29347T>C) in the intron region of sorbin and the SH3 domain-containing 1 (SORBS1) gene were reported to be significantly associated with milk fat traits in cattleyak [46].…”

Section: Discussionmentioning

confidence: 99%

Polymorphisms of CCSER1 Gene and Their Correlation with Milk Quality Traits in Gannan Yak (Bos grunniens)

Yang,

Zhang,

et al. 2023

Foods

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Coiled-coil serine-rich protein 1 (CCSER 1) gene is a regulatory protein gene. This gene has been reported to be associated with various economic traits in large mammals in recent years. The aim of this study was to investigate the association between CCSER1 gene single nucleotide polymorphisms (SNPs) and Gannan yaks and to identify potential molecular marker loci for breeding milk quality in Gannan yaks. We genotyped 172 Gannan yaks using Illumina Yak cGPS 7K liquid microarrays and analyzed the correlation between the three SNPs loci of the CCSER1 gene and the milk qualities of Gannan yaks, including milk fat, protein and casein. It was found that mutations at the g.183,843A>G, g.222,717C>G and g.388,723G>T loci all affected the fat, protein, casein and lactose traits of Gannan yak milk to varying extents, and that the milk quality of individuals with mutant phenotypes was significantly improved. Among them, the milk fat content of AG heterozygous genotype population at g.183,843A>G locus was significantly higher than that of AA and GG genotype populations (p < 0.05); the casein and protein content of mutant GG and CG genotype populations at g.222,717C>G locus was significantly higher than that of wild-type CC genotype population (p < 0.05); and the g.388,723G>T locus of the casein and protein contents of the mutant TT genotype population were significantly higher (p < 0.05) than those of the wild-type GG genotype population. These results provide potential molecular marker sites for Gannan yak breeding.

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Section: Discussionmentioning

confidence: 99%

Polymorphisms of CCSER1 Gene and Their Correlation with Milk Quality Traits in Gannan Yak (Bos grunniens)

Yang,

Zhang,

et al. 2023

Foods

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“…All protocols for collection of the blood and frozen semen samples of experimental individuals were approved by the Institutional Animal Care and Use Committee (IACUC) at China Agricultural University (Permit Number: DK996). The procedures for sampling, laboratory analysis, and data processing were following our previously published article (Li et al, 2019).…”

Section: Animal Ethicsmentioning

confidence: 99%

“…Haplotypes with frequencies >5% were considered distinguishable, whereas the haplotypes with relative frequencies <5% were pooled into a single group (Wang et al, 2013). Detailed procedures were described in our previously published research (Li et al, 2019).…”

Section: Linkage Disequilibrium (Ld) Analysis and Haplotype Constructionmentioning

confidence: 99%

SERPINA1gene identified in RNA-Seq showed strong association with milk protein concentration in Chinese Holstein cows

Liu

Cai

Liu

et al. 2020

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The detection of candidate genes and mutations associated with phenotypic traits is important for livestock animals. A previous RNA-Seq study revealed that SERPINA1 gene was a functional candidate that may affect milk protein concentration in dairy cows. To further confirm the genetic effect of SERPINA1 on milk protein traits, genetic polymorphisms were identified and genotype-phenotype associations were performed in a large Chinese Holstein cattle population. The entire coding region and the 5′-regulatory region (5′-UTR) of SERPINA1 was sequenced using pooled DNA of 17 unrelated sires. Association studies for five milk production traits were performed using a mixed model with a population encompassing 1,027 Chinese Holstein cows. A total of four SNPs were identified in SERPINA1, among which rs210222822 and rs41257068 presented in exons, rs207601878 presented in an intron, and rs208607693 was in the 5′-UTR. Analyses of pairwise D′ measures of linkage disequilibrium (LD) showed strong linkage among these four SNPs (D′ = 0.99–1.00), and a 9 Kb haplotype block involving three main haplotypes with GTGT, CCCC and CCGT was inferred. An association study revealed that all four single SNPs and their haplotypes had significant genetic effects on milk protein percentage, milk protein yield and milk yield (P = 0.0458 − < 0.0001). The phenotypic variance ratio for all 11 significant SNP-trait pairs ranged from 1.01% to 7.54%. The candidate gene of SERPINA1 revealed by our previous RNA-Seq study was confirmed to have pronounced effect on milk protein traits on a genome level. Two SNPs (rs208607693 and rs210222822) presented phenotypic variances of approximately 7% and may be used as key or potential markers to assist selection for new lines of cows with high protein concentration.

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“…SNPs represent changes in only one base of the DNA sequence, i.e., mutations in the nitrogenous bases of the genome sequence (CAETANO, 2009). There has been an increasing use of SNPs in different areas of research, such as human psychiatric disorders (CHAN et al, 2017), milk protein (LI et al, 2019), meat quality (MAGALHÃES et al, 2016), and heat tolerance (DIKMEN et al, 2015). Therefore, SNPs can be used as a selection tool for adaptation to adverse climate conditions (e.g., high temperatures), contributing to minimizing problems related to heat stress in tropical regions (BAENA et al, 2018).…”

Section: 3transcriptomics and Single-nucleotide Polymorphismsmentioning

confidence: 99%

Thermoregulation responses in sheep: a cellular approach

Pantoja¹

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cinco genes diferencialmente expressos (DE) foram upregulated e 10 genes DE foram downregulated no HHT em comparação com o grupo LHT. Foi possível identificar individualidade em relação à capacidade de termorregulação e tolerância ao calor em ovinos Santa Inês no grupo estudado. Estudos mais aprofundados são necessários para entender como a regulação genica se comporta em ovinos deslanados.

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Genetic Analyses Confirm SNPs in HSPA8 and ERBB2 are Associated with Milk Protein Concentration in Chinese Holstein Cattle

Cited by 5 publications

References 40 publications

Polymorphisms of CCSER1 Gene and Their Correlation with Milk Quality Traits in Gannan Yak (Bos grunniens)

Polymorphisms of CCSER1 Gene and Their Correlation with Milk Quality Traits in Gannan Yak (Bos grunniens)

SERPINA1gene identified in RNA-Seq showed strong association with milk protein concentration in Chinese Holstein cows

Thermoregulation responses in sheep: a cellular approach

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