Genetic analyses of restriction fragment length polymorphisms using high molecular weight DNA from sperm or lymphocytes embedded in agarose

Mage, Rose G.; Harindranath, Nagaradona; Hole, N.J.K.; Newman, Barbara A.; Pérez, Rodríguez; Alexander, Cornelius B.; Young-Cooper, Glendowlyn O.

doi:10.1016/0735-0651(88)90002-7

Cited by 11 publications

(4 citation statements)

References 2 publications

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“…Molecular genetic diagnosis can be performed on any accessible body tissue (1)(2)(3) or body fluid (4), even if the biochemical defect is only expressed in a particular inaccessible organ. We focus here on preparation of nucleic acid samples from nucleated blood cells, which is the most readily available and easiest material from which to obtain a reasonable amount of human genomic DNA, when familial investigations are planned for polymorphisms and candidate gene studies.…”

Section: Introductionmentioning

confidence: 99%

DNA Extraction and Stability for Epidemiological Studies

Visvikis¹,

Schlenck²,

Maurice³

1998

CCLM

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During the last few years, an important development of molecular biology techniques has been observed in research and clinical laboratories, and consequently the availability of DNA is becoming essential for epidemiological studies. Several DNA extraction procedures have been proposed. However as the quantity and quality of the DNA extracted is very variable, standardization of the storage of samples, and of extraction procedures becomes essential. Three steps will be considered. (i) Procedures of whole blood preservation prior to extraction which seem to affect yield and purity of the DNA extracted; (ii) DNA extraction procedures, which must be validated by a systematic DNA quality control using DNA molecular weight screening and spectrophotometric analysis, and also by verification that the DNA obtained is well adapted for molecular biology applications; (iii) the third important factor to study is the storage of DNA, since data on short-term (several months) and especially on long-term (several years) DNA stability is often missing and/or conflicting. Detailed studies must be done in order to establish guidelines for proper handling of genetic material.

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Section: Introductionmentioning

confidence: 99%

DNA Extraction and Stability for Epidemiological Studies

Visvikis¹,

Schlenck²,

Maurice³

1998

CCLM

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“…Washings were performed Pharmacia Corp, Piscataway, N.J.). Inserts were treated for 30 min at room temperature in 2 X SSC [22] and 0.1% with 0.5 M EDTA, 1% sodium lauroylsarcosine and 1 SDS, then as needed at 55°C in 0.2 X SSC and 0.1% mg/ml proteinase K, as described [21]. SDS.…”

Section: Rabbitsmentioning

confidence: 99%

Mapping of the duplicated rabbit immunoglobulin χ light chain locus

1991

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The rabbit has two isotypic forms of the immunoglobulin kappa light chain, K1 and K2, which probably arose by duplication. In the normal rabbit, only traces of K2 light chains are produced. However, K2 levels are elevated in allotype-suppressed rabbits and in the Basilea strain which does not produce K1 because of a K1 mRNA splice site mutation. Previous cloning and sequencing showed that each isotype has its own set of J kappa genes but it was not known whether the two isotypes utilize shared or separate sets of V kappa genes. In addition, although genetic linkage of allotypes associated with the K1 and K2 genes has been demonstrated, physical linkage had not been previously demonstrated by overlapping cosmid or phage clones. We used pulsed field and transverse alternating field electrophoresis to obtain megabase maps and to estimate the size of the duplication of the rabbit kappa light chain locus. We found that the two C kappa genes are about 1 megabase apart. One explanation for the poor expression of K2, could be great physical distance from V kappa genes. However, we found that there are V kappa, J kappa and C kappa 2 genes within a approximately 105-kb fragment. Thus, physical distance of V kappa from C kappa 2 may not be the basis for poor K2 expression.

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“…A DNA bio‐bank can be defined as a collection of stable genomic DNA, which enables investigators to perform genetic studies for a long time. DNA can be extracted from almost all body tissues [1–3] and body fluids [4]. Blood is usually the biological material of choice because it is often available in clinical and epidemiological studies and well‐established protocols for DNA extraction with a good yield are available [5].…”

Section: Introductionmentioning

confidence: 99%

Logistics and quality control for DNA sampling in large multicenter studies

Nederhand

Droog

Kluft

et al. 2003

Journal of Thrombosis and Haemostasis

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Summary. To study associations between genetic variation and disease, large bio-banks need to be created in multicenter studies. Therefore, we studied the effects of storage time and temperature on DNA quality and quantity in a simulation experiment with storage up to 28 days frozen, at 4 8C and at room temperature. In the simulation experiment, the conditions did not influence the amount or quality of DNA to an unsatisfactory level. However, the amount of extracted DNA was decreased in frozen samples and in samples that were stored for >7 days at room temperature. In a sample of patients from 24 countries of the EUROPA trial obtained by mail with transport times up to 1 month DNA yield and quality were adequate. From these results we conclude that transport of non-frozen blood by ordinary mail is usable and practical for DNA isolation for polymerase chain reaction in clinical and epidemiological studies.

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Genetic analyses of restriction fragment length polymorphisms using high molecular weight DNA from sperm or lymphocytes embedded in agarose

Cited by 11 publications

References 2 publications

DNA Extraction and Stability for Epidemiological Studies

DNA Extraction and Stability for Epidemiological Studies

Mapping of the duplicated rabbit immunoglobulin χ light chain locus

Logistics and quality control for DNA sampling in large multicenter studies

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