Genetic Analysis and Gene Mapping for a Short-Petiole Mutant in Soybean (Glycine max (L.) Merr.)

Liu, Meifeng; Wang, Yaqi; Gai, Junyi; Bhat, Javaid Akhter; Li, Yawei; Kong, Jiejie; Zhao, Tuanjie

doi:10.3390/agronomy9110709

Cited by 12 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A característica com maior c.v. foi produtividade, isso pode ser explicado, pois a produtividade é uma característica governada por muitos genes e recebe forte influência ambiental. Em relação à produtividade, estudos apontam que a seleção de variedades de soja com alta produtividade está associada a características como maior número de vagens e maior número de grãos por vagem (Kaur et al, 2018;Li et al, 2019). No presente estudo, os tratamentos Check e Elite apresentaram as maiores produtividades, o que pode estar relacionado a essas características.…”

Section: Resultados E Discussõesunclassified

Avanço Da Cultura Da Soja No Tocantins: Período Entre 1990 E 2020

Reis,

Muraishi,

Martins

2023

Estudos Sistemáticos, Concepções E Atualizações Em Ciências Agrárias

View full text Add to dashboard Cite

show abstract

Section: Resultados E Discussõesunclassified

Avanço Da Cultura Da Soja No Tocantins: Período Entre 1990 E 2020

Reis,

Muraishi,

Martins

2023

Estudos Sistemáticos, Concepções E Atualizações Em Ciências Agrárias

View full text Add to dashboard Cite

show abstract

“…The short petiole mutant VP5 was identified from a fast neutron-induced mutant population, and the phenotype of the VP5 mutant was co-segregated with an 837,919 bp deletion fragment on chromosome 17 [ 45 ]. Moreover, the derived short petiole ( dsp ) mutant was controlled by two recessive genes, dsp1 and dsp2 , which were mapped on chromosome seven with flanking markers BARCSOYSSR_07_0787 and BARCSOYSSR_07_0808 and on chromosome 11 between markers BARCSOYSSR_11_0037 and BARCSOYSSR_11_0043, respectively [ 46 ]. Recently, Wang et al [ 47 ] found that the rolled leaves and short petioles mutant ( rlsp1 ) was controlled by multiple genes, and identified 10 candidate regions on chromosomes three, six, eight, thirteen, and seventeen using sequencing-based bulked segregant analysis.…”

Section: Discussionmentioning

confidence: 99%

Combining Fine Mapping, Whole-Genome Re-Sequencing, and RNA-Seq Unravels Candidate Genes for a Soybean Mutant with Short Petioles and Weakened Pulvini

Kong

Zhang

et al. 2022

Genes

View full text Add to dashboard Cite

A short petiole is an important agronomic trait for the development of plant ideotypes with high yields. However, the genetic basis underlying this trait remains unclear. Here, we identified and characterized a novel soybean mutant with short petioles and weakened pulvini, designated as short petioles and weakened pulvini (spwp). Compared with the wild type (WT), the spwp mutant displayed shortened petioles, owing to the longitudinally decreased cell length, and exhibited a smaller pulvinus structure due to a reduction in motor cell proliferation and expansion. Genetic analysis showed that the phenotype of the spwp mutant was controlled by two recessive nuclear genes, named as spwp1 and spwp2. Using a map-based cloning strategy, the spwp1 locus was mapped in a 183 kb genomic region on chromosome 14 between markers S1413 and S1418, containing 15 annotated genes, whereas the spwp2 locus was mapped in a 195 kb genomic region on chromosome 11 between markers S1373 and S1385, containing 18 annotated genes. Based on the whole-genome re-sequencing and RNA-seq data, we identified two homologous genes, Glyma.11g230300 and Glyma.11g230600, as the most promising candidate genes for the spwp2 locus. In addition, the RNA-seq analysis revealed that the expression levels of genes involved in the cytokinin and auxin signaling transduction networks were altered in the spwp mutant compared with the WT. Our findings provide new gene resources for insights into the genetic mechanisms of petiole development and pulvinus establishment, as well as soybean ideotype breeding.

show abstract

“…Another study using soybean breeding line SS98206SP determined that the short petiole trait is controlled by lps3 located in a 12 cM region between markers Sat_234 and Sct_033 in linkage group F (chromosome 13) [7]. Moreover, Liu et al (2019) mapped two recessive loci, dsp1 and dsp2, underlying the short-petiole phenotype to two nonhomologous regions of chromosome 7 and chromosome 11, respectively; 36 and 33 genes were predicted within the physical genomic interval of dsp1 and dsp2, respectively [12].…”

Section: Introductionmentioning

confidence: 99%

Dysfunction of an Anaphase-Promoting Complex Subunit 8 Homolog Leads to Super-Short Petioles and Enlarged Petiole Angles in Soybean

Gao

Zhu

Zhai

et al. 2023

IJMS

View full text Add to dashboard Cite

Plant height, petiole length, and the angle of the leaf petiole and branch angles are crucial traits determining plant architecture and yield in soybean (Glycine max L.). Here, we characterized a soybean mutant with super-short petioles (SSP) and enlarged petiole angles (named Gmssp) through phenotypic observation, anatomical structure analysis, and bulk sequencing analysis. To identify the gene responsible for the Gmssp mutant phenotype, we established a pipeline involving bulk sequencing, variant calling, functional annotation by SnpEFF (v4.0e) software, and Integrative Genomics Viewer analysis, and we initially identified Glyma.11G026400, encoding a homolog of Anaphase-promoting complex subunit 8 (APC8). Another mutant, t7, with a large deletion of many genes including Glyma.11G026400, has super-short petioles and an enlarged petiole angle, similar to the Gmssp phenotype. Characterization of the t7 mutant together with quantitative trait locus mapping and allelic variation analysis confirmed Glyma.11G026400 as the gene involved in the Gmssp phenotype. In Gmssp, a 4 bp deletion in Glyma.11G026400 leads to a 380 aa truncated protein due to a premature stop codon. The dysfunction or absence of Glyma.11G026400 caused severe defects in morphology, anatomical structure, and physiological traits. Transcriptome analysis and weighted gene co-expression network analysis revealed multiple pathways likely involved in these phenotypes, including ubiquitin-mediated proteolysis and gibberellin-mediated pathways. Our results demonstrate that dysfunction of Glyma.11G026400 leads to diverse functional consequences in different tissues, indicating that this APC8 homolog plays key roles in cell differentiation and elongation in a tissue-specific manner. Deciphering the molecular control of petiole length and angle enriches our knowledge of the molecular network regulating plant architecture in soybean and should facilitate the breeding of high-yielding soybean cultivars with compact plant architecture.

show abstract

Genetic Analysis and Gene Mapping for a Short-Petiole Mutant in Soybean (Glycine max (L.) Merr.)

Cited by 12 publications

References 29 publications

Avanço Da Cultura Da Soja No Tocantins: Período Entre 1990 E 2020

Avanço Da Cultura Da Soja No Tocantins: Período Entre 1990 E 2020

Combining Fine Mapping, Whole-Genome Re-Sequencing, and RNA-Seq Unravels Candidate Genes for a Soybean Mutant with Short Petioles and Weakened Pulvini

Dysfunction of an Anaphase-Promoting Complex Subunit 8 Homolog Leads to Super-Short Petioles and Enlarged Petiole Angles in Soybean

Contact Info

Product

Resources

About