Genetic analysis and QTL mapping of a novel reduced height gene in common wheat (Triticum aestivum L.)

Zhou, Chunyun; Xiong, Hui; Li, Yuting; Guo, Huijun; Xie, Yi; Zhao, Liyan; Gu, Jun; Zhao, Shirong; Ding, Yuping; Song, Xiyun; Liu, Luxiang

doi:10.1016/s2095-3119(20)63224-5

Cited by 25 publications

(14 citation statements)

References 40 publications

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“…PH and SC were evaluated in this study. PH and SC were measured at maturity with 8 replicates for each line [29].…”

Section: Plant Materials and Phenotypic Evaluationmentioning

confidence: 99%

Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat

Zhou

Xiong

et al. 2023

Preprint

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Background Plant Height (PH) and Spike Compactness (SC) are important agronomic traits that affect yield improvement in wheat crops. The identification of the loci or genes responsible for these traits is thus of great importance for marker-assisted selection in wheat breeding. Results In this study, we used a RIL population with 139 lines derived from crossing between the mutant Rht8-2 and the local wheat variety NongDa5181 (ND5181) to construct a high-density genetic linkage map by applying the Wheat 40K Panel. We identified 7 stable QTLs for PH (3) and SC (4) under two environments using the RIL population, and found that Rht8-B1 is the causal gene of qPH2B.1 by further genetic mapping, gene cloning and gene editing analyses. Our results further showed that two natural variants from GC to TT in the coding region of Rht8-B1 resulted in an amino acid change from G (ND5181) to V (Rht8-2) at the 175th position, reducing PH by 3.6%~6.2% in the RIL population. Moreover, gene editing analysis suggested that the height of T2 generation in Rht8-B1 edited plants was reduced by 5.6%, and that the impact of Rht8-B1 on PH was significantly lower than Rht8-D1. Additionally, distribution analysis of Rht8-B1 in various wheat resources suggested that Rht8-B1b have not been widely utilized in modern wheat breeding Conclusions The combination of Rht8-B1b with other favorable Rht genes might be an alternative approach for developing lodging-resistant crops. Our study brings important information for marker-assisted selection in wheat breeding.

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“…PH and SC were evaluated in this study. PH and SC were measured at maturity with 8 replicates for each line [29].…”

Section: Plant Materials and Phenotypic Evaluationmentioning

confidence: 99%

Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat

Zhou

Xiong

et al. 2023

Preprint

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“…Recently, a total of 48 QTL controlling spike-related traits located on 18 chromosomes were identified using BSA and the wheat 660 K SNP array in genetic analysis of F 2 and F 2:3 populations [40]. Another example is that a novel reduced height gene was localized on chromosome arm 2DL using BSA-660 K SNP arrays in four F 2 segregating populations [54]. In this study, two low-generation (F 3 ) populations were used to rapidly identify and validate QTL related to KL.…”

Section: The Feasibility Of Qtl Mapping Of Quantitative Traits Using ...mentioning

confidence: 99%

Identification and validation of a major QTL for kernel length in bread wheat based on two F3 biparental populations

Xie

Liu

et al. 2022

BMC Genomics

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Background High yield and quality are essential goals of wheat (Triticum aestivum L.) breeding. Kernel length (KL), as a main component of kernel size, can indirectly change kernel weight and then affects yield. Identification and utilization of excellent loci in wheat genetic resources is of great significance for cultivating high yield and quality wheat. Genetic identification of loci for KL has been performed mainly through genome-wide association study in natural populations or QTL mapping based on genetic linkage map in high generation populations. Results In this study, an F3 biparental population derived from the cross between an EMS mutant BLS1 selected from an EMS-induced wheat genotype LJ2135 (derived from the hybrid progeny of a spelt wheat (T. spelta L.) and a common wheat) mutant bank and a local breeding line 99E18 was used to rapidly identify loci controlling KL based on Bulked Segregant Analysis (BSA) and the wheat 660 K single-nucleotide polymorphism (SNP) array. The highest ratio of polymorphic SNPs was located on chromosome 4A. Linkage map analysis showed that 33 Kompetitive Allele Specific PCR markers were linked to the QTL for KL (Qkl.sicau-BLE18-4A) identified in three environments as well as the best linear unbiased prediction (BLUP) dataset. This QTL explained 10.87—19.30% of the phenotypic variation. Its effect was successfully confirmed in another F3 population with the two flanking markers KASP-AX-111536305 and KASP-AX-110174441. Compared with previous studies and given that the of BLS1 has the genetic background of spelt wheat, the major QTL was likely a new one. A few of predicted genes related to regulation of kernel development were identified in the interval of the detected QTL. Conclusion A major, novel and stable QTL (Qkl.sicau-BLE18-4A) for KL was identified and verified in two F3 biparental populations across three environments. Significant relationships among KL, kernel width (KW) and thousand kernel weight (TKW) were identified. Four predicted genes related to kernel growth regulation were detected in the interval of Qkl.sicau-BLE18-4A. Furthermore, this study laid foundation on subsequent fine mapping work and provided a possibility for breeding of elite wheat varieties.

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“…This approach makes use of allelic segregation and transmission at different loci to construct a genetic linkage map from a large number of molecular markers. A number of mapping examples using linkage analysis have been reported in backcross, double haploid, recombinant inbred lines, near isogenic line, and F 2 populations, initiated with two inbred lines ( Collard et al, 2005 ; Shar et al, 2020 ; Wang et al, 2020 ; Zhou et al, 2020 ). For forest trees, in which no inbred lines are available due to their long-life cycle, large genomes, and high heterozygosity ( White et al, 2007 ), linkage analysis is conducted on the basis of a full-sib family derived from two heterozygous parents ( Maliepaard et al, 1997 ; Wu et al, 2002b ; Lu et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

The Genetic Architecture of Juvenile Growth Traits in the Conifer Torreya grandis as Revealed by Joint Linkage and Linkage Disequilibrium Mapping

Chen

Dong

et al. 2022

Front. Plant Sci.

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Despite its high economical and ornamental values, Torreya grandis, a dioecious non-timber coniferous species, has long been an underrepresented species. However, the advent and application of advanced genotyping technologies have stimulated its genetic research, making it possible to gain new insight into the genetic architecture of complex traits that may not be detected for model species. We apply an open-pollination (OP) mapping strategy to conduct a QTL mapping experiment of T. grandis, in which nearly 100 unrelated trees randomly chosen from the species’ natural distribution and their half-sib progeny are simultaneously genotyped. This strategy allows us to simultaneously estimate the recombination fractions and linkage disequilibrium (LD) coefficients between each pair of markers. We reconstruct a high-density linkage map of 4,203 SNPs covering a total distance of 8,393.95 cM and plot pairwise normalized LD values against genetic distances to build up a linkage-LD map. We identify 13 QTLs for stem basal diameter growth and 4 QTLs for stem height growth in juvenile seedlings. From the linkage-LD map, we infer the evolutionary history of T. grandis and each of its QTLs. The slow decay of QTL-related LDs indicates that these QTLs and their harboring genomic regions are evolutionarily relatively young, suggesting that they can better utilized by clonal propagation rather than through seed propagation. Genetic results from the OP sampling strategy could provide useful guidance for genetic studies of other dioecious species.

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Genetic analysis and QTL mapping of a novel reduced height gene in common wheat (Triticum aestivum L.)

Cited by 25 publications

References 40 publications

Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat

Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat

Identification and validation of a major QTL for kernel length in bread wheat based on two F3 biparental populations

The Genetic Architecture of Juvenile Growth Traits in the Conifer Torreya grandis as Revealed by Joint Linkage and Linkage Disequilibrium Mapping

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