Genetic analysis and quantitative trait locus mapping of PEG‐induced osmotic stress tolerance in cotton

Abstract

Water stress is one of the major abiotic stresses that adversely affect cotton production. Seedlings of 142 backcross inbred lines (BILs) derived from Pima cotton 'Pima S-7' (Gossypium barbadense L.) 9 Upland cotton 'Sure-Grow 747'(G. hirsutum L.) were evaluated in two tests for plant height, fresh shoot weight and root weight under two treatments (5% PEG and water-control conditions) using a hydroponic system in the greenhouse. The experiment in each test was a randomized complete block design with three replicates. The analysis of variance for the two tests detected significant genotypic variation in PEG-induced stress tolerance within the BIL population and between the parents. Heritabilities were moderate to high and were higher under the control conditions than under the PEG treatment, and the three traits were also significantly and positively correlated. Based on a linkage map with 292 loci, six QTLs were detected including two for plant height, and two each for fresh shoot weight and root weight. This study represents the first report in using a permanent mapping population in genetic and linkage analysis of water stress tolerance in cotton.

QTL analysis of agronomic, fiber quality, and abiotic stress tolerance traits in a recombinant inbred population of pima cotton

“…For the drought tolerance study, 10% of polyethylene glycol 8000 (PEG) was used to induce osmotic stress in one hydroponic system as the treatment test following Abdelraheem, Hughs, et al. (2015) and Abdelraheem, Mahdy, and Zhang (2015). For the control test, the RILs and the two parents in the other hydroponic system were irrigated with tap water.…”

“…Pima cotton is expected to be a promising species for abiotic stress (such as drought and salt) tolerance due to it originating from sea coast (Tiwari et al, 2013a(Tiwari et al, , 2013b, but there is a lack of genetic information on abiotic stress tolerance in pima cotton (Flowers, 2004). Drought and salt stresses have a negative impact on cotton growth and development (for reviews, see Abdelraheem, O'Connell, Esmaeili, & Zhang, 2019;Dabbert & Gore, 2014); however, at present, only a few studies have been conducted for abiotic stress tolerance in cotton Abdelraheem, Thyssen, et al, 2018;Abdelraheem, Hughs, Jones, & Zhang, 2015;Abdelraheem, Mahdy, & Zhang, 2015;Adams et al, 2011;Levi et al, 2009;Oluoch et al, 2016;Pauli et al, 2016Pauli et al, , 2017Pauli et al, , 2018Saeed et al, 2011;Saranga, Jiang, Wright, Yakir, & Paterson, 2004;Tiwari et al, 2013aTiwari et al, , 2013bZhang et al, 2009). To our knowledge, no QTL study for abiotic stress tolerance has been reported for American pima cotton.…”

QTL mapping of PEG-induced drought tolerance at the early seedling stage in sesame using whole genome re-sequencing

“…Drought tolerance is a complex quantitative trait in plants. QTL mapping and genome-wide association study (GWAS) have been widely used for genetic analysis of drought tolerance related traits in many plants, such as rice [ 4 , 5 ], wheat [ 6 – 8 ], cotton [ 9 , 10 ] and Brassica napus [ 11 ]. These traits including root length, coleoptile length and shoot length for seedling stage, and yield-related traits for flowering stage.…”

Construction of a High Density Genetic Map and QTL Mapping of PEG-Induced Drought Tolerance at Seedling Stage in Sesame Using Whole Genome Re-Sequencing

“…Drought tolerance is a complex quantitative trait in plants. QTL mapping and genome-wide association study (GWAS) have been widely used for genetic analysis of drought tolerance related traits in many plants, such as rice [4,5], wheat [6][7][8], cotton [9,10] and Brassica napus [11]. These traits including root length, coleoptile length and shoot length for seedling stage, and yield-related traits for owering stage.…”