Genetic analysis and screening of pyrethroid resistance mutations in Varroa destructor populations from Turkey

Koç, Nafiye; İnak, Emre; Jonckheere, Wim; Leeuwen, Thomas Van

doi:10.1007/s10493-021-00626-2

Cited by 13 publications

(8 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resistance development allows insects to survive exposure to the insecticide, however, it often comes with a trade-off that affects the insect's ability to thrive in other aspects of its life cycle or in its natural environment, a phenomenon referred to as “fitness cost” (Kliot and Ghanim 2012 ; Ffrench-Constant and Bass 2017 ). Indeed, a reduction in the number of pyrethroid-resistant Varroa mites in the absence of selection pressure has been documented by Milani and Della Vedova ( 2002 ).…”

Section: Resultsmentioning

confidence: 99%

Geographical distribution of pyrethroid resistance mutations in Varroa destructor across Türkiye and a European overview

Erdem,

Koç-İnak,

Rüstemoğlu

et al. 2024

Exp Appl Acarol

Self Cite

View full text Add to dashboard Cite

Varroa destructor Anderson & Trueman (Acari: Varroidae) is of paramount significance in modern beekeeping, with infestations presenting a primary challenge that directly influences colony health, productivity, and overall apicultural sustainability. In order to control this mite, many beekeepers rely on a limited number of approved synthetic acaricides, including the pyrethroids tau-fluvalinate, flumethrin and organophosphate coumaphos. However, the excessive use of these substances has led to the widespread development of resistance in various beekeeping areas globally. In the present study, the occurrence of resistance mutations in the voltage-gated sodium channel (VGSC) and acetylcholinesterase (AChE), the target-site of pyrethroids and coumaphos, respectively, was examined in Varroa populations collected throughout the southeastern and eastern Anatolia regions of Türkiye. All Varroa samples belonged to the Korean haplotype, and a very low genetic distance was observed based on cytochrome c oxidase subunit I (COI) gene sequences. No amino acid substitutions were determined at the key residues of AChE. On the other hand, three amino acid substitutions, (L925V/I/M), previously associated with pyrethroid resistance, were identified in nearly 80% of the Turkish populations. Importantly, L925M, the dominant mutation in the USA, was detected in Turkish Varroa populations for the first time. To gain a more comprehensive perspective, we conducted a systematic analysis of the distribution of pyrethroid resistance mutations across Europe, based on the previously reported data. Varroa populations from Mediterranean countries such as Türkiye, Spain, and Greece exhibited the highest frequency of resistance mutation. Revealing the occurrence and geographical distribution of pyrethroid resistance mutations in V. destructor populations across the country will enhance the development of more efficient strategies for mite management.

show abstract

Section: Resultsmentioning

confidence: 99%

Geographical distribution of pyrethroid resistance mutations in Varroa destructor across Türkiye and a European overview

Erdem,

Koç-İnak,

Rüstemoğlu

et al. 2024

Exp Appl Acarol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Navajas et al (2010) failed to detect any single nucleotide polymorphisms (SNPs) in CytB within the Korea haplotype of V. destructor parasitizing A. mellifera in Asia, and all the variation was detected with COXI. For the Korea haplotype in Turkey, the genetic distance between V. destructor samples was greatest for COX3 (0.55%), followed by CytB (0.06%), and none for ATP6 (0.00%) (Koçet al, 2021). For V. destructor in Serbia, COXI and CytB revealed SNPs in 51.8 and 16.3% of the samples, respectively (Gajićet al, 2019).…”

Section: Introductionmentioning

confidence: 94%

Varroa destructor and its impacts on honey bee biology

Morfin,

Goodwin,

Guzman-Novoa

2023

Front. Bee Sci.

View full text Add to dashboard Cite

Varroa destructor is considered one of the greatest threats to the health of the honey bee, A. mellifera. In recent years, there has been a considerable increase in the number of studies on the mite and its interaction with the bee at a cellular and molecular level. However, these studies have also revealed just how complex the interaction is. A significant factor in the virulence of V. destructor is the proteins secreted in its saliva, but only a fraction of these have yet been examined. These proteins can negatively affect the bee’s immune system and promote viruses associated with mite parasitism. Initially, studies on parasitized bees concentrated on immune-related genes, but as more genes of the bees have been examined, it is clear that many other aspects of the bee are affected, such as metabolism and neural functioning. Some of those could be responsible for the detrimental changes in certain behaviors of parasitized bees, which compromises the health of the entire colony. Several viruses are associated with V. destructor parasitism, but it remains difficult to distinguish the effects of the viruses from those of the mite. Reduced immunity in parasitized bees also opens the possibility of secondary microbial infections, adding complexity to the mite-bee interaction. Further complicating studies is the impact of other factors, like agrochemicals, which can alter how V. destructor parasitism affects bee immunity, metabolism, and neural functioning. In addition, differences due to age and sex of the bee being parasitized is a factor that needs to be considered in all studies. While much has been learned in recent years about this complex interaction, the number of unanswered questions only increases.

show abstract

“…formic acid, oxalic acid and essential oils (EOs) [ 7 ]. Although synthetic acaricides are easier to use and more effective against varroa mites than organic acaricides, their overuse has led to the emergence of resistance in treated parasites [ 8 , 9 ], and to accumulation of the active ingredients and/or their metabolites in the bee products, with subsequent risk to the consumer [ 10 ]. Moreover, synthetic acaricides may be toxic for both larval and adult bees [ 11 ] or affect the physiology, metabolism and/or behaviour of honey bees [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of natural treatments on the varroa mite infestation levels and overall health of honey bee (Apis mellifera) colonies

Narciso,

Topini,

Ferraiuolo

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

The survival of the honey bee (Apis mellifera), which has a crucial role in pollination and ecosystem maintenance, is threatened by many pathogens, including parasites, bacteria, fungi and viruses. The ectoparasite Varroa destructor is considered the major cause of the worldwide decline in honey bee colony health. Although several synthetic acaricides are available to control Varroa infestations, resistant mites and side effects on bees have been documented. The development of natural alternatives for mite control is therefore encouraged. The study aims at exploring the effects of cinnamon and oregano essential oils (EOs) and of a mixed fruit cocktail juice on mite infestation levels and bee colony health. A multi-method study including hive inspection, mite count, molecular detection of fungal, bacterial and viral pathogens, analysis of defensin-1, hymenoptaecin and vitellogenin immune gene expression, colony density and honey production data, was conducted in a 20-hive experimental apiary. The colonies were divided into five groups: four treatment groups and one control group. The treatment groups were fed on a sugar syrup supplemented with cinnamon EO, oregano EO, a 1:1 mixture of both EOs, or a juice cocktail. An unsupplemented syrup was, instead, used to feed the control group. While V. destructor affected all the colonies throughout the study, no differences in mite infestation levels, population density and honey yield were observed between treatment and control groups. An overexpression of vitellogenin was instead found in all EO-treated groups, even though a significant difference was only found in the group treated with the 1:1 EO mixture. Viral (DWV, CBPV and BQCV), fungal (Nosema ceranae) and bacterial (Melissococcus plutonius) pathogens from both symptomatic and asymptomatic colonies were detected.

show abstract

Genetic analysis and screening of pyrethroid resistance mutations in Varroa destructor populations from Turkey

Cited by 13 publications

References 54 publications

Geographical distribution of pyrethroid resistance mutations in Varroa destructor across Türkiye and a European overview

Geographical distribution of pyrethroid resistance mutations in Varroa destructor across Türkiye and a European overview

Varroa destructor and its impacts on honey bee biology

Effects of natural treatments on the varroa mite infestation levels and overall health of honey bee (Apis mellifera) colonies

Contact Info

Product

Resources

About