Genetic analysis of a Piezo-like protein suppressing systemic movement of plant viruses in Arabidopsis thaliana

Zhang, Zhen; Tong, Xin; Liu, Songyu; Chai, Long-Xiang; Zhu, Feifan; Zhang, Xiao-Peng; Zou, Jing-Ze; Wang, Xianbing

doi:10.1038/s41598-019-39436-3

Cited by 47 publications

(36 citation statements)

References 52 publications

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“…Genome‐wide screens of Saccharomyces cerevisiae (yeast) replicating brome mosaic virus (BMV) (Kushner et al , ) or tomato bushy stunt virus (TBSV) (Panavas et al , ) showed that a compatible host contains both pro‐viral and antiviral factors that affect virus replication at the cellular level. Mutagenesis and genetic analyses in Arabidopsis thaliana (Arabidopsis) allowed identification of pro‐viral and antiviral factors that affect virus replication at the cellular level, cell‐to‐cell and systemic movement (Diaz‐Pendon et al , ; Garcia‐Ruiz et al , ; Guo et al , ; Lellis et al , ; Zhang et al , ). Accordingly, viruses need pro‐viral host factors and are targeted by antiviral host factors.…”

Section: Host Determinants Of Infectionmentioning

confidence: 99%

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Host factors against plant viruses

García-Ruíz

2019

Molecular Plant Pathology

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Plant virus genome replication and movement is dependent on host resources and factors. However, plants respond to virus infection through several mechanisms, such as autophagy, ubiquitination, mRNA decay and gene silencing, that target viral components. Viral factors work in synchrony with pro-viral host factors during the infection cycle and are targeted by antiviral responses. Accordingly, establishment of virus infection is genetically determined by the availability of the pro-viral factors necessary for genome replication and movement, and by the balance between plant defence and viral suppression of defence responses. Sequential requirement of pro-viral factors and the antagonistic activity of antiviral factors suggest a two-step model to explain plant-virus interactions. At each step of the infection process, host factors with antiviral activity have been identified. Here we review our current understanding of host factors with antiviral activity against plant viruses.

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Section: Host Determinants Of Infectionmentioning

confidence: 99%

“…Plants encode mechanosensitive ion channels that regulate ion movement across cells. In Arabidopsis, ESC1 encodes a piezo protein that functions as a mechanosensitive Ca 2+ permeable channel and limits systemic infection of CMV and TuMV (Zhang et al , ).…”

Section: Host Genes With Antiviral Activitymentioning

confidence: 99%

Host factors against plant viruses

García-Ruíz

2019

Molecular Plant Pathology

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“…To construct Arabidopsis thaliana ccp mutant lines, two 19 bp sequences (see Supplementary Table S1 at JXB online) specifically targeting the 5' coding region of CCP were cloned into the pHEC401 vector as described previously ( Xing et al , 2014 ; Zhang et al , 2019 ). All primers used for plasmid constructions are listed in Supplementary Table S1 , and all the plasmids were verified by sequencing.…”

Section: Methodsmentioning

confidence: 99%

“…Bacterial growth was assessed in three independent experiments. For virus infection assay, three fully grown leaves of 4-week-old Arabidopsis plants were mechanically inoculated with Cucumber mosaic virus (CMV; 20 ng μl –1 ) as described previously ( Zhang et al , 2019 ). Systemically infected leaves were collected for RNA analyses.…”

Section: Methodsmentioning

confidence: 99%

A putative nuclear copper chaperone promotes plant immunity in Arabidopsis

Chai

Dong

Liu

et al. 2020

Journal of Experimental Botany

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Copper is essential for many metabolic processes but must be sequestrated by copper chaperones. It is well known that plant copper chaperones regulate various physiological processes. However, functions of copper chaperones in plant nucleus remain largely unknown. Here, we identified a putative Copper Chaperone induced by Pathogens (CCP) in Arabidopsis thaliana. CCP harbors a classical MXCXXC Cu binding site (CBS) at its N terminus and a nuclear localization signal (NLS) at the C terminus. CCP mainly formed nuclear speckles in the plant nucleus, which requires the NLS and CBS domains. Overexpression of CCP induced PR1 expression and enhanced resistance against Pseudomonas syringae pv. Tomato DC3000 (Pst DC3000) compared with Col-0 plants. Conversely, two CRISPR/Cas9-mediated ccp mutants were impaired in plant immunity. Further biochemical analyses revealed that CCP interacted with the transcription factor TGA2 in vivo and in vitro. Moreover, CCP recruits TGA2 to the PR1 promoter sequences in vivo, which induces defense gene expression and plant immunity. Collectively, our results have identified a putative nuclear copper chaperone required for plant immunity and provided evidence for potential function of Cu in the SA pathway.

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“…Given that PIEZOs play prominent roles in multiple aspects of animal mechanosensation and physiology [16][17][18][19] , we investigated the role of AtPIEZO in plant mechanosensation. A recent study reported that AtPIEZO regulated virus translocation within the plant, but its specific role in mechanotransduction was not addressed 20 . Here we use genetic tools, electrophysiological methods and calcium imaging to investigate the role of AtPIEZO in root mechanosensation.…”

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confidence: 99%

PIEZO ion channel is required for root mechanotransduction inArabidopsis thaliana

Mousavi

Dubin

Zeng

et al. 2020

Preprint

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SummaryPlant roots adapt to the mechanical constraints of the soil to grow and absorb water and nutrients. As in animal species, mechanosensitive ion channels in plants are proposed to transduce external mechanical forces into biological signals. However, the identity of these plant root ion channels remains unknown. Here, we show that Arabidopsis thaliana PIEZO (AtPIEZO) has preserved the function of its animal relatives and acts as an ion channel. We present evidence that plant PIEZO is highly expressed in the columella and lateral root cap cells of the root tip which experience robust mechanical strain during root growth. Deleting PIEZO from the whole plant significantly reduced the ability of its roots to penetrate denser barriers compared to wild type plants. piezo mutant root tips exhibited diminished calcium transients in response to mechanical stimulation, supporting a role of AtPIEZO in root mechanotransduction. Finally, a chimeric PIEZO channel that includes the C-terminal half of AtPIEZO containing the putative pore region was functional and mechanosensitive when expressed in naive mammalian cells. Collectively, our data suggest that Arabidopsis PIEZO plays an important role in root mechanotransduction and establishes PIEZOs as physiologically relevant mechanosensitive ion channels across animal and plant kingdoms.

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Genetic analysis of a Piezo-like protein suppressing systemic movement of plant viruses in Arabidopsis thaliana

Cited by 47 publications

References 52 publications

Host factors against plant viruses

Host factors against plant viruses

A putative nuclear copper chaperone promotes plant immunity in Arabidopsis

PIEZO ion channel is required for root mechanotransduction inArabidopsis thaliana

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