Genetic analysis of African swine fever virus in Bali based on p72 and CD2v Genes

Abstract

African Swine Fever (ASF) has caused negative effects in the swine industry in various countries of the world, including Indonesia. Bali is one of the most severely affected provinces in Indonesia. Phylogenetic study to determine the genotype and serogroup of the ASF virus found in Bali has never been done before. In this study, virus was obtained from an infected spleen sample, designated as Bali/2023, and two gene fragments, namely p72 (B646L) and CD2v (EP402R), were then amplified by PCR and sequenced by Sanger method. The DNA sequences of p72 and CD2v virus was then compared with sequences from other countries for genetic analysis. The results showed that Bali/2023 virus is genetically very close to viruses found in other Asian countries, mainly viruses isolated in China and Vietnam in 2019. These genetic analyses results placed Bali/2023 virus in genotype II and serogroup 8.