Genetic analysis of an N-ethyl-N-nitrosourea-induced mutation at the hemoglobin β-chain locus in mice

Murota, Tetsuro; Shibuya, Tohru; Tutikawa, K

doi:10.1016/0165-7992(82)90162-2

Cited by 5 publications

(4 citation statements)

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“…These mutations were heritable and viable under homozygous conditions. One of the mutants was observed as a change in the mobility of the bands on electrophoresis (Murota et al 1982) . The mobility of the bands was similar to that of Pon which carrier Hbb alleles under homozygous conditions (Watanabe et al 1976).…”

Section: Resultsmentioning

confidence: 99%

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Genetic analysis of mutants in mice induced by N-ethyl-N-nitrosourea.

Murota

Shibuya

1983

The Japanese journal of genetics

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Specific locus tests of the effects of N-ethyl-N-nitrosourea (ENU) on mouse spermatogonia using visible loci and hemoglobin beta-chain (Hbb) locus have been performed.Male wild-type (C3Hf/He) mice were injected intraperitoneally with ENU. Eight weeks after injection, the males were mated with tester strain females (PW) homozygous for six visible recessive genes. Ten visible and 2 hemoglobin mutants were obtained.The mutation frequency was 60.3 x 10-5/locus/generation. These mutations were all heritable, and nine out of ten mutations were viable under homozygous conditions. The results suggest that the mutations induced by ENU on spermatogonia of mice might have resulted from events at the genic rather than the chromosomal level.

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Section: Resultsmentioning

confidence: 99%

“…The preparation of samples for electrophoretic studies was described elsewhere (Murota et al, 1982). The genotypes of C3Hf /He and PW were Hbbd/Hbbd and Hbb'/Hbb', respectively.…”

Section: Methodsmentioning

confidence: 99%

Genetic analysis of mutants in mice induced by N-ethyl-N-nitrosourea.

Murota

Shibuya

1983

The Japanese journal of genetics

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“…1981 Murota et al, 1982;Murota and Shibuya, 1983;1991;Shibuya et al, 1993;1996;Murota, 199519721972198919801985Mutation Research 1977MMS 19881960FDA in vivo 19701977 54…”

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Achievements of the late Kiyoshi Tutikawa, a mouse geneticist

Kikuchi¹

2004

Environ. Mutagen Res.

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“…Experiments with x-rays and triethylenemelamine have yielded four a-thalassemias and one tandem gene duplication involving the ,B-globin locus (11,12). Two more mutations induced by the mutagen ethylnitrosourea (EtNU) have been described, one mutation causing an electrophoretic mobility change in a-hemoglobin (13,14) and the other causing altered mobility of the d-minor component of /3-globin (15). Others are currently being analyzed (16).…”

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confidence: 99%