Genetic analysis of bovine viral diarrhea virus in pre-weaned native Korean calves

Ryu, Jae Hyoung; Choi, Kwang Shik

doi:10.1007/s11250-019-01882-6

Cited by 5 publications

(2 citation statements)

References 22 publications

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“…Although several studies have been conducted on the detection of viral pathogens associated with CD in the Republic of Korea (ROK) (Park et al 2018;Park et al 2007a;Park et al 2011;Park et al 2007b;Park et al 2008;Ryu and Choi 2019), there is little available information about the association between diarrhea and viral pathogens. The objective of the present study was to investigate the prevalence of BCoV, BNoV, BoRVA, and BToV in calves aged 1-60 days with or without diarrhea and the relationships between calf age and viral pathogens as well as to report any associations between these pathogens and diarrhea in calves.…”

Section: Introductionmentioning

confidence: 99%

Molecular surveillance of viral pathogens associated with diarrhea in pre-weaned Korean native calves

Ryu

Shin

Choi

2020

Trop Anim Health Prod

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Calf diarrhea causes severe economic losses in the cattle industry worldwide. This study investigated the prevalence of bovine coronavirus (BCoV), bovine norovirus (BNoV), bovine group A rotavirus (BoRVA), and bovine torovirus (BToV) in calves aged ≤ 60 days, regardless of diarrhea, across nine different regions in the Republic of Korea (ROK) and reported associations between these viruses and diarrhea. Fecal samples were collected rectally from 689 calves: normal (n = 360) and diarrheic (n = 329). BNoV (84/689, 12.2%) was the most prevalent regardless of diarrhea, followed by BCoV (37/689, 5.4%), BToV (15/689, 2.2%), and BoRVA (13/689, 1.9%). Although BCoV (P = 0.032) and BoRVA (P = 0.007) were significantly associated with diarrhea in pre-weaned calves, BNoV and BToV showed no association. Infection by the four pathogens had no relationship with calf age; BoRVA was detected only in calves aged < 30 days, but this finding was not statistically significant. Phylogenetic analysis revealed that BCoV isolates obtained in this study were distinct from the other known BCoVs, and all BNoV isolates belonged to GIII.2 genotype; genetic variations in BNoVs are present in the ROK. BoRVA isolates distributed in the ROK were assigned to G6P[5]. Within the P[5] genotype, our isolates were divided into two lineages: P[5]-III and P[5]-VIII. P[5]-VIII lineage was dominant in pre-weaned Korean native calves. Our BToV isolates were more closely related to a European isolate, B145, than to Japanese isolates. Here, BNoV was frequently identified in calves, suggesting its non-significant contribution to calf diarrhea, whereas BCoV and BoRVA were responsible for calf diarrhea in the ROK. Consequently, our results highlight the importance of rapid diagnosis against these viruses in calf diarrhea.

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Section: Introductionmentioning

confidence: 99%

Molecular surveillance of viral pathogens associated with diarrhea in pre-weaned Korean native calves

Ryu

Shin

Choi

2020

Trop Anim Health Prod

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“…7 Furthermore, diverse BVDV subtypes have been reported in Korea. 7,21 In a retrospective study conducted in Korea, BVDV was a major enteric and reproductive pathogen, with a higher frequency of detection in native Korean cattle than in Holstein cattle. 16 These results might contribute to the high frequency of BVDV detection in the bovine abortion cases in our study.…”

Section: Discussionmentioning

confidence: 99%

Laboratory investigation of causes of bovine abortion and stillbirth in the Republic of Korea, 2014–2020

Kim,

Lee

et al. 2024

J VET Diagn Invest

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Bovine abortion is a critical problem in the cattle industry. Identifying causes of abortion is key to establishing appropriate herd management and prevention strategies. We used pathology examinations, detection of etiologic agents, and serology to determine the cause of bovine abortions in Korea. We analyzed 360 abortion and stillbirth cases submitted to the Animal and Plant Quarantine Agency from December 2014 to January 2020. The putative cause of abortion was identified in 140 of 360 (38.9%) cases; 124 of the 140 (88.6%) cases were attributed to infections. The most common etiologic agents detected were bovine viral diarrhea virus (65 of 360; 18.1%), Coxiella burnetii (19 of 360; 5.3%), Leptospira spp. (13 of 360; 3.6%), Listeria monocytogenes (9 of 360; 2.5%), and Neospora caninum (8 of 360; 2.2%). Minor abortifacient pathogens included Brucella abortus (2 of 360; 0.6%), bovine alphaherpesvirus 1 (2 of 360; 0.6%), Akabane virus (2 of 360, 0.6%), and bovine ephemeral fever virus (1 of 360; 0.3%). Non-infectious conditions included congenital anomalies (7 of 360; 1.9%), goiter (7 of 360; 1.9%), and vitamin A deficiency (2 of 360; 0.6%). Our diagnostic rate in cases with placenta submitted (42 of 86; 48.8%) was significantly higher than in cases without placenta (98 of 274; 35.8%), which highlights the value of submitting placentas. Our results confirm the status of the large variety of causative agents associated with abortions in cattle in Korea.

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Molecular detection and genotyping of bovine viral diarrhea virus in four provinces of China

Liu,

Zhou,

Wang

et al. 2024

Preprint

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Bovine viral diarrhea virus (BVDV) has long been one of the major pathogens hindering the development of the global beef industry. In the present study, to further investigate the epidemic profile and genetic diversity of the virus, 77 fecal samples were collected from cattle with diarrhea in Henan, Sichuan, Shandong and Hebei provinces of China during 2023–2024 and screened for the presence of BVDV using reverse transcription-polymerase chain reaction (RT-PCR). The positive samples for BVDV were subjected to the cloning and phylogenetic analysis of the 5ʹ-UTR and whole-genome sequences. The results showed that 35 out of 77 (35/77, 45.45%) bovine diarrhea samples tested positive for BVDV, with the highest proportion rate being observed in Henan province at 57.14% (20/35). Twenty 5ʹ-UTR and one whole-genome sequences of BVDV strains were acquired and analyzed. Phylogenetic analysis of the 5ʹ-UTR sequences indicated that 20 strains belonged to the BVDV-1m subtype, while the BVDV-385 strain from Henan province was clustered within the BVDV-3 genotype, indicating that the BVDV-1m subtype strains were the main strains circulating in China. Further amino acid analysis showed that a unique aa deletion and ten unique aa substitutions were exhibited in the E2 protein of the BVDV-385 strain, while seven unique aa sites in the NS5B protein. Furthermore, linear B cell epitope prediction indicated that four linear B cell potential epitopes were altered in the E2 protein, while predictions of N-glycosylation sites (NGS) revealed an additional glycosylation site (574 NPS) in the NS5B protein. Overall, this study reported the BVDV-3 strain was found in Henan Province, China, and performed a genetic evolutionary analysis of its whole genome sequence, which had important implications for understanding the genetic diversity of ongoing BVDV cases

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Genetic analysis of bovine viral diarrhea virus in pre-weaned native Korean calves

Cited by 5 publications

References 22 publications

Molecular surveillance of viral pathogens associated with diarrhea in pre-weaned Korean native calves

Molecular surveillance of viral pathogens associated with diarrhea in pre-weaned Korean native calves

Laboratory investigation of causes of bovine abortion and stillbirth in the Republic of Korea, 2014–2020

Molecular detection and genotyping of bovine viral diarrhea virus in four provinces of China

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