Genetic analysis of Dermatophilus spp. using multilocus enzyme electrophoresis

Trott, Darren J.; Masters, Anne M.; Carson, J. M.; Ellis, T.M.; Hampson, David J.

doi:10.1016/s0934-8840(11)80793-0

Cited by 17 publications

(4 citation statements)

References 17 publications

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“…The results of cultural, morphological, and biochemical studies, including restriction enzyme analysis of bacterial DNAs and analysis of the PAGE patterns of bacterial proteins (see above), together with the genetic distances obtained in the study of Trott et al (34), indicate that the chelonid isolates and D. congolensis strains belong to different species. We propose the name Dermatophilus chelonae for the chelonid isolates.…”

Section: Discussionmentioning

confidence: 87%

“…Differs from D. congolensis by electrophoretic detection of alleles for the following enzymes (34): phosphoglucose mutase, 6-phosphoglucose dehydrogenase, arginophosphokinase, catalase, superoxide dismutase, phosphoglucose isomerase, malate dehydrogenase, malic enzyme, nucleoside phosphorylase, leucine amino peptidase, and lactate dehydrogenase.…”

Section: Discussionmentioning

confidence: 99%

“…Restriction enzyme analysis with ApaI, BamHI, and PvuII was performed as described by Sambrook et al (27). These restriction enzymes were chosen because they differentiated groups of D. congolensis isolates in other studies (9,34). Digests were electrophoresed in 0.7% Ultra pure DNA grade agarose (Bio-Rad Laboratories) in Tris-borate-EDTA buffer at 2.7 V/cm for 4 h. The gels were stained with a solution containing 0.1 pg of ethidium bromide per ml and were photographed with Polaroid type 667 film under UV light.…”

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confidence: 99%

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Dermatophilus chelonae sp. nov., Isolated from Chelonids in Australia

Masters

Ellis

Carson

et al. 1995

International Journal of Systematic Bacteriology

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Three isolates of a previously undescribed Dermatophilus sp. obtained from chelonids (two strains obtained from turtles and one strain obtained from a tortoise) were compared with 30 Dermatophilus congolensis isolates obtained from Australian mammals. The microscopic appearance, the colony morphology, and most biochemical test results for the chelonid isolates were characteristic of the genus Dermatophilus. Our isolates differed from the mammalian D. congolensis isolates in a number of cultural characteristics, including faster growth at 27°C than at 37"C, formation of two hemolysis zones around colonies on blood agar at 37°C in the presence of 10% CO,, poor motility, and production of a distinctive odor. The DNA restriction enzyme digestion and protein electrophoresis patterns of our strains were distinct. The electrophoretic mobilities of 11 enzymes differed from the mobilities observed with D. congolensis strains. A monoclonal antibody to a surface antigen of an ovine isolate did not react with zoospores or filaments of the chelonid isolates. Biochemical differences between our isolates and D. congolensis included the ability of the chelonid isolates to reduce nitrate to nitrite and the fact that the chelonid isolates exhibit collagenase activity in vitro. We propose that the chelonid isolates should be placed in a new species, Dermatophilus chelonae. Strain W16, which was isolated from a nose scab on a snapping turtle, is the type strain; a culture of this strain has been deposited in the American Type Culture Collection as strain ATCC 51576.A skin disease caused by the actinomycete Dermatophilus congolensis affects a wide variety of animal species and occurs throughout the world. This disease is economically important in cattle in tropical regions and in sheep in high-rainfall areas. Dermatophilosis lesions have also been found in goats, horses, deer, gazelles, giraffes, foxes, chamois, Columbian ground squirrels, cottontail rabbits, owl monkeys, polar bears, seals, pigs, and humans (13). To date, there apparently have been no reports of isolations of D. congolensis from chelonids, although the organism has been isolated from lizards (2,23,30). It has been reported that there is considerable variation in the colony morphology and cultural characteristics of D. congolensis (12). Significant variations in serological, biochemical, and virulence characteristics within the species have also been documented (1,8,10,12,16,18,19,21,33). A study of the variations in the cultural, biochemical, and morphological properties of Australian mammalian isolates of D. congolensis was recently undertaken (9). In our study we compared the properties of three isolates obtained from chelonids with the properties of these mammalian D. congolensis isolates. MATERIALS AND METHODSBacterial isolates. Dermatophilus sp. isolates were obtained from an abscess in a tortoise in 1980 (isolate WS), from skin lesions on a turtle in 1982 (isolate Wll), and from a nose scab on a snapping turtle in 1987 (isolate W16= [T = type strain]); all of...

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Dermatophilus chelonae sp. nov., Isolated from Chelonids in Australia

Masters

Ellis

Carson

et al. 1995

International Journal of Systematic Bacteriology

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“…Of the methods available for typing bacteria, MLEE has been used widely and many studies have demonstrated its utility for providing information on the genetic structure of bacterial populations. [13][14][15][16][17][18][19][20][21][22] However, MLEE has not been applied to S intermedius. This paper describes the use of MLEE to determine the genetic structure of S intermedius from normal skin and from a variety of disease conditions in dogs.…”

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Use of multilocus enzyme electrophoresis to distinguish clinically important strains of Staphylococcus intermedius from the skin of dogs

et al. 2000

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The small difference in genetic diversity between isolates from the skin and mucosa of healthy dogs and isolates from various diseases, as well as the presence of the majority of isolates in two ETs, is consistent with the role of S intermedius as an opportunistic pathogen. The confinement of all Staphylococcal Scalded Skin Syndrome isolates within one ET is confirmation of this entity as a distinct disease of dogs.

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The Family Dermatophilaceae

Stackebrandt¹

2006

The Prokaryotes

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Genetic analysis of Dermatophilus spp. using multilocus enzyme electrophoresis

Cited by 17 publications

References 17 publications

Dermatophilus chelonae sp. nov., Isolated from Chelonids in Australia

Dermatophilus chelonae sp. nov., Isolated from Chelonids in Australia

Use of multilocus enzyme electrophoresis to distinguish clinically important strains of Staphylococcus intermedius from the skin of dogs

The Family Dermatophilaceae

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