Genetic Analysis of Drosophila melanogaster X-Chromosome Neurodegenerative Mutants Induced by Ethyl Methansulfonate and Nitrosoethylurea

Shcherbata, Halyna R.; Matiytsiv, N. P.; Chernyk, Y. I.; Yatsenko, Andriy S.; Radysh, V. V.; Kucherenko, M. M.; Maksymiv, D. V.

doi:10.1023/b:ruge.0000041388.04203.1b

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…line, we revealed a diferent phenotype with small vacuoles in all brain regions, which is not a hallmark of sws [13] (Figure 1C and H). Therefore, we believe that it is a new allele of sws, and we used deiciency mapping on 7D1-D5 band (Df (1) C128) which uncovered a sws phenotype [9].…”

Section: −15mentioning

confidence: 91%

“…During the massive screen tests searching for X-linked mutants with age-dependent neurodegeneration, we analyzed parain-embedded histological sections of the brain tissue in mutant lies with life span reduction [13]. Sections from a number of mutants showed strong brain vacuolization similar to sws phenotype described by Krezschmar et al [9].…”

Section: The New Sws Allelementioning

confidence: 99%

“…All mutants were isolated in screens for structural brain defects using the histology brain assay [38]: sws 1 is previously described by Krezschmar et al [9], sws 76−15 (also referred as 76-15 line) isolated by Shcherbata et al [13]. Another strain was obtained from the Bloomington Drosophila Stock Centre (Indiana University, USA).…”

Section: Melanogaster Linesmentioning

confidence: 99%

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Swiss Cheese, Drosophila Ortholog of Hereditary Spastic Paraplegia Gene NTE, Maintains Neuromuscular Junction Development and Microtubule Network

Ryabova¹,

Matiytsiv²,

Trush³

et al. 2018

Drosophila Melanogaster - Model for Recent Advances in Genetics and Therapeutics

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Neuropathy target esterase (NTE) is a molecular target for the organophosphorus compound-induced delayed neuropathy (OPIDN) and also one of the genetic factors responsible for the development of the hereditary spastic paraplegia (HSP), characterized by axon degeneration of motoneurons causing progressive lower-limb spastic paralysis. Both HSP and OPIDN are characterized by the distal axonopathy. The molecular mechanisms underlying the axonopathy involved in HSP and OPIDN are poorly understood. In order to have a beter understanding of the mechanisms that NTE is involved in, we used one of the homologs, human NTE. Swiss cheese (sws) is a Drosophila melanogaster ortholog of NTE with 39% homology. Mutations in sws as it was shown before lead to age-dependent neurodegeneration, structure alteration of glia cells, and reduced insect life span. To study SWS functions, we used the system of the third-instar larval neuromuscular junctions of D. melanogaster. In this study, we show that mutations in sws (sws 1 and sws 76−1) and SWS knockdown alter neuromuscular junction's morphology and synaptic microtubules organization.

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Section: −15mentioning

confidence: 91%

Section: The New Sws Allelementioning

confidence: 99%

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Swiss Cheese, Drosophila Ortholog of Hereditary Spastic Paraplegia Gene NTE, Maintains Neuromuscular Junction Development and Microtubule Network

Ryabova¹,

Matiytsiv²,

Trush³

et al. 2018

Drosophila Melanogaster - Model for Recent Advances in Genetics and Therapeutics

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Genetic analysis of Drosophila melanogaster chromosome 3 neurodegenerative mutants induced with ethyl methanesulfonate

et al. 2009

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Locomotor activity of Drosophila melanogaster larvae with altered function of gene swiss cheese

Matiytsiv¹,

Trush²,

Gudyma³

et al. 1970

Fakt. eksp. evol. org.

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Aim. Gene swiss cheese (sws) in Drosophila melanogaster is ortholog of human gene Neuropathy Target Esterase (NTE). Point mutation in this gene lead to many neurodegenerative disorders, such as Spastic Paraplegia. The aim of this study was to determine whether the change of gene sws function influence in Drosophila larvae motor behavior. Methods. We used two mutant lines: sws1 and sws76-15; transgenic lines for UAS-Gal4 controlled expression of SWS in moroneurons. Locomotor assay was performed in third-instar larvae. Results. We have found that larvae sws76-15 and sws1 showed significant decrease of motor behavior in comparison with wild type: sws1 has 35.4 %, and sws76-15 24.1 % difference with Oregon R. Functional knockout of sws in motoneuron showed decrease of motor activity as well – 16.5 % less than control. overexpression sequences of complete SWS protein in motoneurons causes significant increment in motor activity about 43.7 %. Esterase domen and three nucleotidbinding domains overexpression in larvae motoneurons had no differences compared to control. Conclusions. For the first time was shown a critical role of gene sws for larvae motor activity in D. melanogaster. Flies with altered sws function are good model of spastic paraplegia study. Keywords: Drosophila melanogaster, sws/NTE, neuropathy, locomotor behavior.

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Genetic Analysis of Drosophila melanogaster X-Chromosome Neurodegenerative Mutants Induced by Ethyl Methansulfonate and Nitrosoethylurea

Cited by 3 publications

References 8 publications

Swiss Cheese, Drosophila Ortholog of Hereditary Spastic Paraplegia Gene NTE, Maintains Neuromuscular Junction Development and Microtubule Network

Swiss Cheese, Drosophila Ortholog of Hereditary Spastic Paraplegia Gene NTE, Maintains Neuromuscular Junction Development and Microtubule Network

Genetic analysis of Drosophila melanogaster chromosome 3 neurodegenerative mutants induced with ethyl methanesulfonate

Locomotor activity of Drosophila melanogaster larvae with altered function of gene swiss cheese

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