Genetic analysis of embryos, obtained from vitrificated oocytes in donor’s programs of artificial reproductive technologies

Abstract

The factors of cryopreservation can disrupt molecular mechanisms of normal chromosome’s segregation during meiosis and to increase the number of aneuploidy in embryos. The aim of the paper was to determine the frequency of aneuploid embryos obtained from the thawed oocytes in comparison with the fresh embryo cycles. Preimplantation genetic testing for aneuploidy was performed using fluorescence in situ hybridization with the study of such chromosomes as 13, 16, 18, 21, 22, X, Y. Statistical hypotheses were checked using criteria t, c2 at levels of significance p<0.05, p<0.01, p<0.001. The results have shown that the embryos obtained from vitrificated and fresh oocytes had identical frequency of chromosomal nondisjunctions. However, there was marked the increasing of the mosaicism frequency in the embryos get from thawed oocytes. Aneuploidies were rarer determine among the embryos obtained from vitrificated oocytes, which may indicate a less viability of oocytes with violations in the number of certain chromosomes. This research is the fragment of SRW IPCandC NASU 2.2.6.58 “Study of influence of cryopreservation factors by the vitrification method on morphologically functional descriptions of reproductive cells and embryos”, number of state registration 0116u003498.