Genetic analysis of fruit quality traits in prebred lines of watermelon derived from a wild accession of Citrullus amarus

Maragal, Siddharood; Rao, Eguru Sreenivasa; Reddy, D. C. Lakshmana

doi:10.1007/s10681-019-2527-x

Cited by 15 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…found that the round fruit shape is due to a 159-bp deletion in the coding region of Cla011257 [74]. This nding was con rmed by Maragal et al (2019) and Legendre et al (2020) who reported also a novel allele at this locus [75][76].…”

Section: Qtl For Fruit Size and Shape In Watermelonmentioning

confidence: 68%

QTL Mapping of Fruit and Seed-related Traits in Watermelon Using Genotyping-by-Sequencing-based High-density Linkage Mapping

Gao

Liang

Liu³

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundWatermelon is an important vegetable crop with dual use of both fruit and seeds. Understanding the genetic basis of fruit quality and seed size-related traits is important for efficient marker-assisted breeding in watermelon. Linkage mapping in watermelon segregating populations using genotyping-by-sequencing (GBS) provides insights into genetic control of fruit- and seed-related traits and genome collinearity in commercial watermelon cultivars. ResultsIn the present study, we conducted QTL mapping of 12 horticulturally important traits on external and internal fruit quality and seed size/weight using segregating populations derived a cross between two commercial varieties. A high-density genetic map was developed with GBS which contained more than 6,000 SNP loci in 1,004 bins with a total map length of 1261.1 cM and average marker interval of 1.26 cM or 329 kb. Phenotypic data of fruit rind color (RC), rind stripe pattern (RSP), flesh color (FFC), fruit diameter (FD), fruit length (FL), fruit shape index (FSI), fruit weight (FW), Brix content central (BCC), Brix content edge (BCE), seed length (SL), width (SW), and weight (20SWT) were collected from two locations in two years. QTL analysis identified 47 QTL for the 12 traits, of which 24 had moderate- or major-effects, and 34 were novel QTL not identified in previous studies. The QTL for RSP were identified overlapped with previous reports, and mapped the QTL to a small interval on chromosome 6. From the detected novel QTL, we identify FD (qfd2.1), FL (qfl2.1) co-located with FSI (qfsi2.1) QTL on chromosome 2, and the minor QTL qfw3.2 co-located with previously reported fruit shape QTL (qfd3.1, qfl3.1, qfsi3.1), and SW (qsw10.1) co-located with 20SWT QTL (q20swt10.1) on chromosome 10, and 5 minor QTL (qbcc2.1, qbcc5.1, qbce2.1, qbce2.2, qbce5.1) were found to be likely new locus for Brix content.ConclusionWe conducted GBS consisting of 120 F2 individuals and developed a high-density linkage map with more than 6,000 SNP loci in 1004 bins in watermelon. We identified 47 QTL for 12 fruit and seed related traits including 34 novel QTL. Our work expands the molecular breeding toolbox for watermelon to improve the yield and fruit quality.

show abstract

Section: Qtl For Fruit Size and Shape In Watermelonmentioning

confidence: 68%

QTL Mapping of Fruit and Seed-related Traits in Watermelon Using Genotyping-by-Sequencing-based High-density Linkage Mapping

Gao

Liang

Liu³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Fruit size (length, width, and shape) variations in watermelon have been classified into elongated, round, blocky, or oval shapes ( McKay, 1936 ; Wehner et al., 2001 ). Many studies have identified QTLs that control the majority of fruit variation in various biparental mapping populations, RILs, and natural populations under various environmental conditions ( Lu et al., 2009 ; Sandlin et al., 2012 ; Ren et al., 2014 ; Liu et al., 2014 ; Kim et al., 2015 ; Liu et al., 2015 ; Liu et al., 2016 ; Cheng et al., 2016 ; Lu et al., 2016 ; Dou et al., 2018b ; Maragal et al., 2019 ; Legendre et al., 2020 ). The inclusive number of QTLs regulating the watermelon shape/size have been reviewed across all previously published QTL results ( Pan et al., 2020 ), and 9 inclusive QTLs have been reported across 7 distinct chromosomal regions of Chr-02, Chr-03, Chr-05, Chr-07, Chr-08, Chr-09, and Chr-10.…”

Section: Discussionmentioning

confidence: 99%

“…But, the quantitative genetics of watermelon FSI variations have been similarly stated ( Gusmini and Wehner, 2005 ; Gusmini and Wehner, 2007 ; Kumar and Wehner, 2013 ), e.g., the genetic locus “ClFSI-3.2” harboring the O gene has been significantly validated with the homologous SUN gene in tomato ( Dou et al., 2018b ). Recently, a newly identified allelic fashion revealed that the rounded watermelon shape is produced by the deletion of a 159 bp region in the CDS coding sequence of the Cla011257 gene ( Maragal et al., 2019 ; Legendre et al., 2020 ). In our study, we also identified few more genes for FL, FW, and FSI, but strong validation should be required for validation.…”

Section: Discussionmentioning

confidence: 99%

Primary mapping of quantitative trait loci regulating multivariate horticultural phenotypes of watermelon (Citrullus lanatus L.)

Amanullah

Osae

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Watermelon fruits exhibit a remarkable diversity of important horticultural phenotypes. In this study, we initiated a primary quantitative trait loci (QTL) mapping to identify the candidate regions controlling the ovary, fruit, and seed phenotypes. Whole genome sequencing (WGS) was carried out for two differentiated watermelon lines, and 350 Mb (96%) and 354 Mb (97%) of re-sequenced reads covered the reference de novo genome assembly, individually. A total of 45.53% non-synonymous single nucleotide polymorphism (nsSNPs) and 54.47% synonymous SNPs (sSNPs) were spotted, which produced 210 sets of novel SNP-based cleaved amplified polymorphism sequence (CAPS) markers by depicting 46.25% co-dominant polymorphism among parent lines and offspring. A biparental F2:3 mapping population comprised of 100 families was used for trait phenotyping and CAPS genotyping, respectively. The constructed genetic map spanned a total of 2,398.40 centimorgans (cM) in length and averaged 11.42 cM, with 95.99% genome collinearity. A total of 33 QTLs were identified at different genetic positions across the eight chromosomes of watermelon (Chr-01, Chr-02, Chr-04, Chr-05, Chr-06, Chr-07, Chr-10, and Chr-11); among them, eight QTLs of the ovary, sixteen QTLs of the fruit, and nine QTLs of the seed related phenotypes were classified with 5.32–25.99% phenotypic variance explained (PVE). However, twenty-four QTLs were identified as major-effect and nine QTLs were mapped as minor-effect QTLs across the flanking regions of CAPS markers. Some QTLs were exhibited as tightly localized across the nearby genetic regions and explained the pleiotropic effects of multigenic nature. The flanking QTL markers also depicted significant allele specific contributions and accountable genes were predicted for respective traits. Gene Ontology (GO) functional enrichment was categorized in molecular function (MF), cellular components (CC), and biological process (BP); however, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were classified into three main classes of metabolism, genetic information processing, and brite hierarchies. The principal component analysis (PCA) of multivariate phenotypes widely demonstrated the major variability, consistent with the identified QTL regions. In short, we assumed that our identified QTL regions provide valuable genetic insights regarding the watermelon phenotypes and fine genetic mapping could be used to confirm them.

show abstract

“…ClSUN25-26-27a (gene ID: Cla011257) conditions an elongated fruit shape in watermelon [15,16]. Maragal et al [57] reported that Cla011257 regulates fruit shape. Fruit shape is defined based on the fruit shape index (FSI), which is the ratio of the fruit length (FL) to the fruit diameter (FD).…”

Section: Fruit Shapementioning

confidence: 99%

Retrospective Genetic Analysis of Qualitative and Quantitative Traits in Sweet Watermelon (Citrullus lanatus var. lanatus): A Review

et al. 2022

View full text Add to dashboard Cite

Understanding the genetic basis of a crop’s qualitative and quantitative traits is vital to designing market preferred varieties. The aim of this review is to present a retrospective genetic analysis of qualitative and quantitative phenotypic traits in sweet watermelon as a guide for trait integration and the development of novel varieties with yield potential and desirable horticultural attributes. The first section outlines genes conditioning the inheritance of plant architecture (e.g., leaf attributes and plant architecture), floral characters (flowering rate, sex expression, and male sterility), fruit traits (shape, colour, rind colour and stripe patterns and flesh colour) and seed morphology (seed length, width, size and coat colour). In the second section, developments in molecular markers and quantitative trait loci (QTL) to aid marker-assisted breeding are discussed. Further, the review highlights the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology and its scope in gene manipulations and new variety development. The information presented in this review is useful for optimised and demand-led breeding to develop new varieties to serve growers, consumers and the sweet watermelon industry.

show abstract

Genetic analysis of fruit quality traits in prebred lines of watermelon derived from a wild accession of Citrullus amarus

Cited by 15 publications

References 27 publications

QTL Mapping of Fruit and Seed-related Traits in Watermelon Using Genotyping-by-Sequencing-based High-density Linkage Mapping

QTL Mapping of Fruit and Seed-related Traits in Watermelon Using Genotyping-by-Sequencing-based High-density Linkage Mapping

Primary mapping of quantitative trait loci regulating multivariate horticultural phenotypes of watermelon (Citrullus lanatus L.)

Retrospective Genetic Analysis of Qualitative and Quantitative Traits in Sweet Watermelon (Citrullus lanatus var. lanatus): A Review

Contact Info

Product

Resources

About