Genetic Analysis of Genes Controlling Natural Variation of Seed Coat and Flower Colors in Soybean

Yang, Kiwoung; Jeong, Namhee; Moon, Jung-Kyung; Lee, Yeong-Ho; Lee, Suk-Ha; Kim, Hwan Mook; Hwang, Cheol Ho; Back, Kyoungwhan; Palmer, Reid G.; Jeong, Soon‐Chun

doi:10.1093/jhered/esq078

Cited by 86 publications

(117 citation statements)

References 46 publications

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“…The variety of seed coat and flower colors of soybean accessions observed in this study could complement previous study in respect to genetic analysis of genes controlling natural variation of the traits in a soybean population (Yang et al, 2010). The brown seed coat color shown in Table 1 is actually the imperfect black and, therefore, is classified as the black seed coat color.…”

Section: Morphological Character Performances Of 56 Soybean Acessionssupporting

confidence: 71%

Characterization of 56 Soybean Accessions on Yield Components and Maturity-related Traits

Tasma¹,

Lestari²,

Reflinur³

2016

BPN

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ABSTRAKPeningkatan produktivitas kedelai nasional dapat dilakukan dengan penggunaan varietas produktivitas tinggi dan manipulasi indeks panen menggunakan varietas genjah. Pembentukan varietas di atas memerlukan plasma nutfah dengan potensi hasil tinggi dan berumur genjah. Tujuan dari penelitian ini adalah untuk mengidentifikasi aksesi kedelai yang mempunyai potensi hasil tinggi dan aksesi kedelai berumur genjah. Sebanyak 56 aksesi kedelai terdiri atas varietas elit dan introduksi, aksesi lokal dan galur-galur persilangan ditanam di Kebun Percobaan Cikeumeuh (250 m dpl) dan Pacet (1.200 m dpl) menggunakan Rancangan Acak Kelompok dengan tiga ulangan. Pengamatan dilakukan terhadap karakter morfologi, komponen hasil dan karakter reproduktif yang meliputi umur berbunga (fase R1, R3, R7, dan R8). Hasil penelitian menunjukkan 28 aksesi (50%) diuji di Cikeumeuh dan 43 aksesi (76,79%) diuji di Pacet menunjukkan jumlah polong/tanaman lebih dari 50. Sekitar 35,71% aksesi di Cikeumeuh dan 41,07% aksesi di Pacet menghasilkan biji/tanaman lebih dari 10 g/tanaman. Aksesi kedelai yang menunjukkan komponen hasil tinggi hanya di KP Cikeumeuh adalah B2981, B3517, dan B3628. Aksesi kedelai yang menunjukkan komponen hasil tinggi hanya di KP Pacet adalah B4441, B3628, B382, B4334, dan B3414. Aksesi yang menunjukkan komponen hasil tinggi di kedua lokasi (Cikeumeuh dan Pacet) adalah B3417. Aksesi B3417 diklasifikasikan sebagai aksesi dengan adaptasi luas karena berkeragaan komponen hasil tinggi di dataran rendah dan di dataran tinggi. Aksesi dengan umur panen genjah ditunjukkan oleh B2973 (74 hari setelah tanam, hst) yang tidak berbeda nyata dengan aksesi B1430 (75 hst), B3611 (76 hst), B4433 (77 hst), dan B4439 (80 hst) berdasarkan uji DMRT (p<0,05). Berdasarkan karakter morfologi, sebagian besar plasma nutfah kedelai terbagi dalam tiga kelompok termasuk landraces tanpa mempertimbangkan asal daerahnya. Analisis klaster berdasarkan karakter agronomi mendukung analisis DMRT bahwa aksesi kedelai dengan hasil biji tinggi dan umur genjah dapat dibedakan. Aksesi kedelai dengan jumlah polong banyak, hasil biji tinggi, umur genjah potensial digunakan dalam program pemuliaan kedelai produktivitas tinggi dengan umur genjah.Kata kunci: komponen hasil, umur genjah, plasma nutfah, aksesi, kedelai. ABSTRACTOne effort to improve soybean production in Indonesia is by using high yielding and manipulating harvest index by using early maturing varieties. Such variety development requires the availability of soybean germplasm with high yield potential and early maturity. The objective of this study was to identify soybean genotypes showing high yield potential and early maturity. A total of 56 soybean accessions consisting of elite and introducing varieties, landraces, and breeding lines were characterized in the field with different altitudes i.e. Cikeumeuh (250 m above sea level) and Pacet (1.200 m asl). The experiments were arranged in a randomized block design using three replications. Characters observed were morphological chracters, yield component...

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Section: Morphological Character Performances Of 56 Soybean Acessionssupporting

confidence: 71%

Characterization of 56 Soybean Accessions on Yield Components and Maturity-related Traits

Tasma¹,

Lestari²,

Reflinur³

2016

BPN

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“…In contrast, seed coats with high anthocyanin content exhibit a dark-red color. In soybean (Glycine max), the recessive i allele, which releases CHS from silencing, results in high anthocyanin accumulation in the seed coat, which can be dark brown or even black depending on other loci such as R and T (Tuteja et al, 2004;Yang et al, 2010). In contrast, the dominant I allele, which silences CHS expression and hence blocks both anthocyanin and PA biosynthesis, results in a completely colorless seed coat.…”

Section: Myb5 and Myb14 Physically Interact And Synergisticallymentioning

confidence: 99%

MYB5 and MYB14 Play Pivotal Roles in Seed Coat Polymer Biosynthesis in Medicago truncatula

2014

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In Arabidopsis (Arabidopsis thaliana), the major MYB protein regulating proanthocyanidin (PA) biosynthesis is TT2, named for the transparent testa phenotype of tt2 mutant seeds that lack PAs in their coats. In contrast, the MYB5 transcription factor mainly regulates seed mucilage biosynthesis and trichome branching, with only a minor role in PA biosynthesis. We here characterize MYB5 and MYB14 (a TT2 homolog) in the model legume Medicago truncatula. Overexpression of MtMYB5 or MtMYB14 strongly induces PA accumulation in M. truncatula hairy roots, and both myb5 and myb14 mutants of M. truncatula exhibit darker seed coat color than wild-type plants, with myb5 also showing deficiency in mucilage biosynthesis. myb5 mutant seeds have a much stronger seed color phenotype than myb14. The myb5 and myb14 mutants accumulate, respectively, about 30% and 50% of the PA content of wild-type plants, and PA levels are reduced further in myb5 myb14 double mutants. Transcriptome analyses of overexpressing hairy roots and knockout mutants of MtMYB5 and MtMYB14 indicate that MtMYB5 regulates a broader set of genes than MtMYB14. Moreover, we demonstrate that MtMYB5 and MtMYB14 physically interact and synergistically activate the promoters of anthocyanidin reductase and leucoanthocyanidin reductase, the key structural genes leading to PA biosynthesis, in the presence of MtTT8 and MtWD40-1. Our results provide new insights into the complex regulation of PA and mucilage biosynthesis in M. truncatula.

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“…The single nucleotide polymorphism (SNP) and insertion and deletion (InDel) primer sequences are shown in Fig. 1a (Jeong et al 2004;Rafalski 2002;Yang et al 2010). The expected product sizes were 252 for SNP and 131/134 bp for InDel (Fig.…”

Section: Plant Materials and Molecular Analysismentioning

confidence: 99%

Development of Molecular Markers for Low Raffinose and Stachyose in Korean Soybean Cultivars

Yang

et al. 2014

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT A novel allele of the putative soybean raffinose synthase gene, RS2, was discovered in PI200508 that is associated with the low raffinose and stachyose content. Soybean line PI200508 was identified as expressing reduced levels of raffinose and stachyose as well as elevated levels of sucrose. The RS2 mutant gene shows three base pairs InDel with the normal gene. Based on InDel region we developed novel co-dominant and dominant marker. The aim of this study was to develop Korean soybean cultivars, Daewon, Cheongja, and Danmiput, containing low levels of raffinose and stachyose. A specific markers assay for the PI200508 RS2 allele was developed to allow direct selection of the low raffinose and stachyose phenotype. Our findings highlight the efficiency of allele-specific markers in selection, which is evident in the matching genotype and results of the HPLC in the F2 generations of Daewon×PI200508 population.

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Genetic Analysis of Genes Controlling Natural Variation of Seed Coat and Flower Colors in Soybean

Cited by 86 publications

References 46 publications

Characterization of 56 Soybean Accessions on Yield Components and Maturity-related Traits

Characterization of 56 Soybean Accessions on Yield Components and Maturity-related Traits

MYB5 and MYB14 Play Pivotal Roles in Seed Coat Polymer Biosynthesis in Medicago truncatula

Development of Molecular Markers for Low Raffinose and Stachyose in Korean Soybean Cultivars

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