Genetic analysis of hordein polypeptides from single seeds of barley

Abstract

A simple rapid electrophoretic method for non-destructive analysis of hordeins in barley seeds was used to show that observed gel patterns are probably determined by pairs of co-dominant alleles at two linked loci 10-16 recombination units apart.

Genetics of CM-proteins (A-hordeins) in barley

“…Solari and Favret (1971) located a number of loci controlling hordein polypeptides in a segment of chromosome 5. More recent work (Oram et al 1975;Shewry et al 1978Shewry et al , 1980Netsvetaev 1978;Sozinov et al 1978;Dolí and Brown 1979;Jensen et al 1980) has led to the characterization of two complex loci, Horl and Hor2, which encode C and B-hordeins, respectively, and are located in the short arm of chromosome 5. Lawrence and Shepherd (1981), using wheat-barley addition and translocation lines, located the gene for D-hordein in the long arm of the same chromosome, a finding which has been fully confirmed with the characterization of the Hor3 locus by Shewry et al (1983).…”

Amino acid sequences of hordein polypeptides

“…One distinguishes the C polypeptides with an apparent molecular weight ranging from 45.000 to 70.000 and the B polypeptides ranging from 25.000 to 40.000 (14). These two groups of polypeptides are considered to be coded for by two different loci on chromosome 5 (12,19,23,24). In a recent communication (21) methods for the purification of individual hordein polypeptides have been reported and it was found that the hordein polypeptides C2 and B I differed in the amino acid composition of the total chain as well as their carboxy-terminal sequences.…”

Cloning of double stranded DNA coding for hordein polypeptides

“…From the present results it is expected that separate messenger RNA molecules and consequently separate genes code for the 8-10 identified B and C-hordein polypeptides. The present and additional sequences transcribed and cloned from isolated hordein messenger RNA molecules can be used to explore how many genes are present in the hor-2 region of chromosome 5 (12,22,24,26) coding for the Bhordein polypeptides. Analogously cloned sequences for the C-hordein polypeptides can be used to analyse the hor-1 region of chromosome 5 considered to code for the C-hordein polypeptides.…”