Genetic analysis of <i>Lolium.</i> I. Identification of linkage groups and the establishment of a genetic map

Hayward, M. D.; Forster, John W.; Jones, James T. A.; Dolstra, O.; Evans, Clive; McAdam, N. J.; Hossain, Khwaja; Stammers, M.; Will, Jutta; Humphreys, Mervyn O.; Evans, Gareth M.

doi:10.1111/j.1439-0523.1998.tb01972.x

Cited by 60 publications

(45 citation statements)

References 26 publications

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“…Disturbance of expected S and Z allele ratios and linked markers has been suggested in Lolium (Hayward et al, 1998) and in rye (Philipp et al, 1994) based on the hypothesis that some S or Z alleles, or SZ allele combinations, are less effective than others at preventing self-pollination. However, in the present study, both male and female gametes that form progeny from the original fully compatible cross are equally likely to contain either of the two segregating S and Z alleles.…”

Section: Discussionmentioning

confidence: 99%

Self-incompatibility in ryegrass 12. Genotyping and mapping the S and Z loci of Lolium perenne L

et al. 2002

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Perennial ryegrass (Lolium perenne L.) is an outcrossing, wind-pollinated species exhibiting a gametophytic two-locus system of self-incompatibility (S and Z). The two incompatibility loci were genotyped in a cross between a doubled-haploid plant crossed as the female parent with a normal heterozygous plant. The S and Z loci were found to segregate in the expected 1:1 ratio and also segregated independently. The two loci were mapped to linkage groups one and two respectively, in accordance with the Triticeae consensus map. In addition, there were notable associations

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Section: Discussionmentioning

confidence: 99%

Self-incompatibility in ryegrass 12. Genotyping and mapping the S and Z loci of Lolium perenne L

et al. 2002

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“…An approach that simplifies the cross structure is to mate a single heterozygous individual with a homozygous or near-homozygous tester (a one-way pseudo-testcross; Ritter et al 1990;De Simone et al 1997), to give a population containing a maximum of two different combinations of alleles at a single locus and four at two loci. The first genetic map for perennial ryegrass was based on such a population (p129) derived from the cross between a L. perenne × L. multiflorum interspecific hybrid and an artificial homozygote produced by anther culture (Hayward et al , 1998. The map contains 106 loci, of which, 34 are restriction fragment length polymorphisms (RFLPs) detected by Lolium cDNA and genomic DNA probes and seven are RFLPs detected with heterologous probes from oat and wheat.…”

Section: Introductionmentioning

confidence: 99%

An enhanced molecular marker based genetic map of perennial ryegrass (Lolium perenne) reveals comparative relationships with other Poaceae genomes

Jones¹,

Mahoney²,

Hayward³

et al. 2002

Genome

210

238

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A molecular-marker linkage map has been constructed for perennial ryegrass (Lolium perenne L.) using a one-way pseudo-testcross population based on the mating of a multiple heterozygous individual with a doubled haploid genotype. RFLP, AFLP, isoenzyme, and EST data from four collaborating laboratories within the International Lolium Genome Initiative were combined to produce an integrated genetic map containing 240 loci covering 811 cM on seven linkage groups. The map contained 124 codominant markers, of which 109 were heterologous anchor RFLP probes from wheat, barley, oat, and rice, allowing comparative relationships between perennial ryegrass and other Poaceae species to be inferred. The genetic maps of perennial ryegrass and the Triticeae cereals are highly conserved in terms of synteny and colinearity. This observation was supported by the general agreement of the syntenic relationships between perennial ryegrass, oat, and rice and those between the Triticeae and these species. A lower level of synteny and colinearity was observed between perennial ryegrass and oat compared with the Triticeae, despite the closer taxonomic affinity between these species. It is proposed that the linkage groups of perennial ryegrass be numbered in accordance with these syntenic relationships, to correspond to the homoeologous groups of the Triticeae cereals.Key words: Lolium perenne, genetic linkage map, RFLP, AFLP, conserved synteny.Résumé : Une carte génétique composée de marqueurs moléculaires a été produite pour l'ivraie vivace (Lolium perenne L.) à l'aide d'une population issue d'un pseudo-testcross unidirectionnel. Les parents étaient, d'une part, un individu multiple hétérozygote et, d'autre part, un génotype haploïde doublé. Des données pour des marqueurs RFLP, AFLP, isoenzymatiques ainsi que des EST ont été contribuées par quatre laboratoires faisant partie du « International Lolium Genome Initiative (ILGI) ». Ces données ont été combinées pour produire une carte génétique intégrée comprenant 240 locus, formant sept groupes de liaison et s'étendant sur 811 cM. La carte compte 124 marqueurs codominants dont 169 sont des sondes-repères RFLP hétérologues provenant du blé, de l'orge, de l'avoine ou du riz. Ces sondes permettent d'examiner les relations entre l'ivraie vivace et d'autres espèces de graminées. Les cartes génétiques de l'ivraie vivace et des céréales de la tribu des hordées sont très conservées en termes de synténie et de colinéarité. Cette conclusion s'appuie sur une concordance générale au niveau des relations de synténie entre l'ivraie vivace, l'avoine et le riz ainsi qu'entre les hordées et ces espèces. Un plus faible niveau de synténie et de colinéarité a été observé entre l'ivraie vivace et l'avoine par rapport aux hordées, malgré la proximité taxinomique entre ces espèces. Il est suggéré que les groupes de liaison chez l'ivraie vivace soient numérotés en fonction des relations de synténie de façon à correspondre aux groupes d'homéologues chez les céréales.

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“…Moreover, molecular markers such as restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) (Hayward et al, 1994;Hayward et al, 1998), amplified fragment length polymorphism (AFLP) (Bert et al, 1999), and simple sequence repeat (SSR) markers (Jones et al, 2001;Jones et al, 2002) were used to examine population genetic structure and diversity, and cultivar identification. They were also used to correlate molecular findings with morpho-agronomical traits (Geleta et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers

Ghariani¹,

Elazreg²,

Chtourou-Ghorbel³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Tunisia is rich in diverse forage and pasture species including perennial ryegrass. In order to enhance forage production and improve agronomic performance of this local germplasm, a molecular analysis was undertaken. Random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and morpho-agronomical traits markers were used for genetic diversity estimation of ryegrass germplasm after screening 20 spontaneous accessions, including a local and an introduced cultivars. Same mean polymorphism information content values were obtained (0.37) for RAPD and ISSR suggesting that both marker systems were equally effective in determining polymorphisms. The average pairwise genetic distance values were 0.57 (morpho-agronomical traits), 0.68 (RAPD), and 0.51 (ISSR) markers data sets. A higher Shannon diversity index was obtained with ISSR marker (0.57) than for RAPD (0.54) and morphoagronomical traits (0.36). The Mantel test based on genetic distances of a combination of molecular markers and morpho-agronomical data exhibited (2015) a significant correlation between RAPD and ISSR data, suggesting that the use of a combination of molecular techniques was a highly efficient method of estimating genetic variability levels among Tunisian ryegrass germplasm. In summary, results showed that combining molecular and morpho-agronomical markers is an efficient way in assessing the genetic variability among Tunisian ryegrass genotypes. In addition, the combined analysis provided an exhaustive coverage for the analyzed diversity and helped us to identify suitable accessions showed by Beja and Jendouba localities, which present large similarities with cultivated forms and can be exploited for designing breeding programmes, conservation of germplasm and management of ryegrass genetic resources.

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Genetic analysis of Lolium. I. Identification of linkage groups and the establishment of a genetic map

Cited by 60 publications

References 26 publications

Self-incompatibility in ryegrass 12. Genotyping and mapping the S and Z loci of Lolium perenne L

Self-incompatibility in ryegrass 12. Genotyping and mapping the S and Z loci of Lolium perenne L

An enhanced molecular marker based genetic map of perennial ryegrass (Lolium perenne) reveals comparative relationships with other Poaceae genomes

Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers

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