Genetic analysis of <i>Toxocara</i> spp. in stray cats and dogs in Van province, Eastern Turkey

Oğuz, Bekir; Özdal, Nalan; Deger, M. Serdar

doi:10.2478/jvetres-2018-0042

Cited by 12 publications

(8 citation statements)

References 22 publications

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“…Morphological examination of specimens in the present work was in agreement with description of T. cati ( 15 , 22 , 23 ). In the past, for identification of a Toxocara species, the ordinary method of is determination of morphological characters.…”

Section: Discussionsupporting

confidence: 88%

“…might be confusing and mistaken ( 1 , 13 , 14 ). Felines are infected by T. cati, T. malyesensis, Toxoascaris leonine, and occasionally T. canis ( 13 , 15 ), so the identification of Ascaridid nematodes infecting cats become more complicated and requires more precise tools. Molecular based methods in identification, discrimination of Toxocara spp .…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and Molecular Characterization of Toxocara cati Infection in Feral Cats in Alexandria City, Northern Egypt

El-Seify

Marey²,

Satour³

et al. 2021

IJPA

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Background: This study was performed to determine the prevalence and to identify precisely Toxocara spp., which infects feral cats in Alexandria, Egypt based on morphological and molecular approaches. Methods: This cross-sectional study was carried out on 100 feral cats trapped from different areas of Alexandria during 2018. Adult male and female worms were recovered from small intestinal contents after euthanasia and dissection of cats. Distinct morphological features were initially determined using available keys, and then after amplification and sequencing of the mitochondrial NADH dehydrogenase subunit 1 (nad1) gene was carried out and phylogenetic trees were constructed. Results: Forty out of 100 cats were infected with Toxocara spp. Intensity of infection ranged from 1 to 9 worms/cat, with a mean of 2.27±1.6. All isolates were confirmed as T. cati based on morphological features and the sequence of nad1 gene. Results of the current study clearly show that Egyptian T. cati isolate examined herein is genetically similar to those recorded in other countries. Conclusion: The current work revealed high prevalence of T. cati in feral cats in the study area. This is the first genetic study that confirms T. cati from feral cats in Egypt. In addition, it demonstrated the suitability and need of genetic markers such as nad1 for identification of Toxocara spp. Furthermore highlights the public health importance of T. cati in Egypt.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Toxocara cati Infection in Feral Cats in Alexandria City, Northern Egypt

El-Seify

Marey²,

Satour³

et al. 2021

IJPA

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“…Additionally, PCR has also been used in some studies as a sensitive technique for the diagnosis of parasitosis in cats. 20,21 T cati PCR was negative in this case, leaving the question of the identity of the helminth unanswered. However, the size of the eggs present in our specimen was similar to the size of the eggs of T cati (62.3-72.7 µm).…”

Section: Discussionmentioning

confidence: 89%

Common bile duct obstruction caused by a helminth in a cat in the UK: ultrasonographic findings, histopathology and outcome

Venier¹,

Compagnone²,

Kerins³

et al. 2021

Journal of Feline Medicine and Surgery Open Reports

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Case summary An 11-year-old neutered female domestic shorthair cat presented to our hospital with a 5-day history of vomiting, lethargy, anorexia and hyperbilirubinaemia, despite intravenous fluid therapy, gastroprotectants and antibiotic treatment. An abdominal ultrasound revealed a markedly distended common bile duct (diameter 6.2 mm). The cystic duct and intrahepatic bile ducts were also dilated. A linear structure formed by two parallel hyperechoic lines was identified in the common bile duct and could be traced to the duodenal papilla. The cat underwent laparotomy for surgical decompression of the biliary tree. A tubular, brown-coloured structure was retrieved from the common bile duct. Histological examination was consistent with a degenerate helminth. The cat recovered uneventfully from the surgery and its demeanour and appetite improved rapidly over the following days. Liver and gallbladder wall histopathology was consistent with bacterial cholangitis and cholecystitis. Escherichia coli was cultured from both bile and liver parenchyma. Relevance and novel information To our knowledge, this is the first reported case of extrahepatic biliary duct obstruction caused by a helminth in a cat in the UK. We hypothesised that the obstruction had been caused by the aberrant migration of an intestinal nematode that became lodged in the duodenal papilla. Ultrasound allowed prompt diagnosis and guided the treatment decision.

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“…With difficulty in proving this on the human level, the present study directed toward determine molecular and phylogenetic compatibility between them. The recent studies in Turkey by Oguz and Oguz et al (2018) demonstrate the value of the mitochondrial cytochrome c oxidase subunit I (cox1) gene of T. vitulorum from cattle calves and ITS-2 fragment of T. canis from dogs were used to identify the morphological gene in identification, respectively. In this respect, DNA-based approaches have been applied to identify the nematode species, mainly using ITS fragments (rDNA) Li et al (2006) and Fogt-Wyrwas et al (2013).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, studies by Mikaeili et al (2015) in Iran and Oguz (2018) in Turkey demonstrate the value of the mitochondrial cytochrome c oxidase subunit I (cox1) gene in the identification of T. vitulorum from cattle calves. Regarding Oguz et al (2018), existing keys and PCR sequencing of the ITS-2 fragment were used to identify the phylogenetic tree of T. canis from stray dogs in Turkey. With significant variations in pathogenicity and destitution of T. vitulorum infection between cattle and buffalo calves in Egypt, no previous studies in this respect concerning the genomic difference between T. vitulorum extracted from two different hosts; cattle and buffalo as well as its computability or differences with that extracted from dogs.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt

et al. 2020

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SummaryToxocara canis of dogs and Toxocara vitulorum of cattle and buffalo are nematode parasites that cause serious economic and public health problems all over the world. This study aims to provide molecular data to identify and distinguish between Toxocara spp. from dogs, cattle and buffalo in Egypt. Moreover, constructing a phylogeny and phylogenetic relationships among these Toxocara spp. were performed through an analytic study of ATPase-6, a mitochondrial gene; 12S, small subunit ribosomal RNA gene and ITS-2, the second internal transcribed spacer nuclear ribosomal gene. T. vitulorum from cattle and buffalo were found to be almost identical. The ATPase- 6 and 12S regions showed 87.78 % and 90.38 % nucleotide similarity between T. canis and T. vitulorum, while for the ITS-2 region, only 78.38 % was found. Analysis of the three studied genes revealed that each Toxocara spp. has distinct molecular characteristics. Moreover, it was revealed that these genes, especially the ITS-2 gene, are useful and sensitive molecular markers for classifying and studying the phylogenetic analysis and relationships among closely related Toxocara spp. All sequences obtained in this study were registered in the GenBank under the accession numbers: MG214149 -MG214157.

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Genetic analysis of Toxocara spp. in stray cats and dogs in Van province, Eastern Turkey

Cited by 12 publications

References 22 publications

Prevalence and Molecular Characterization of Toxocara cati Infection in Feral Cats in Alexandria City, Northern Egypt

Prevalence and Molecular Characterization of Toxocara cati Infection in Feral Cats in Alexandria City, Northern Egypt

Common bile duct obstruction caused by a helminth in a cat in the UK: ultrasonographic findings, histopathology and outcome

Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt

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