Genetic analysis of Indian tasar silkmoth (Antheraea mylitta) populations

Chakraborty, Saikat; Muthulakshmi, M.; Vardhini, Deena; Jayaprakash, P.; Nagaraju, Javaregowda; Arunkumar, Kallare P.

doi:10.1038/srep15728

Cited by 18 publications

(7 citation statements)

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“…A detailed study on the genetic analysis of Indian tasar silk moth ( A. mylitta ) populations has been published [ 78 ]. However, no detailed information is available for A. mylitta cocoonase gene.…”

Section: Discussionmentioning

confidence: 99%

In silico structural and functional characterization of Antheraea mylitta cocoonase

Sneha

Pandey

2022

Journal of Genetic Engineering and Biotechnology

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Background Cocoonase is a serine protease present in sericigenous insects and majorly involved in dissolving of sericin protein allowing moth to escape. Cocoon structure is made up of sericin protein which holds fibroin filaments together. Cocoonase enzyme hydrolyzes sericin protein without harming the fibroin. However, until date, no detailed characterization of cocoonase enzyme and its presence in wild silk moth Antheraea mylitta has been carried out. Therefore, current study aimed for detailed characterization of amplified cocoonase enzyme, secondary and tertiary structure prediction, sequence and structural alignment, phylogenetic analysis, and computational validation. Several computational tools such as ProtParam, Iterative Threading Assembly Refinement (I-TASSER), PROCHECK, SAVES v6.0, TM-align, Molecular Evolutionary Genetics Analysis (MEGA) X, and Figtree were employed for characterization of cocoonase protein. Results The present study elucidates about the isolation of RNA, cDNA preparation, PCR amplification, and in silico characterization of cocoonase from Antheraea mylitta. Here, total RNA was isolated from head region of A. mylitta, and gene-specific primers were designed using Primer3 followed by PCR-based amplification and sequencing. The newly constructed 377-bp length sequence of cocoonase was subjected to in silico characterization. In silico study of A. mylitta cocoonase showed 26% similarity to A. pernyi strain Qing-6 cocoonase using Blastp and belongs to member of chymotrypsin-like serine protease superfamily. From phylogenetic study, it was found that A. mylitta cocoonase sequence is closely related to A. pernyi cocoonase sequence. Conclusions The present study revealed about the detailed in silico characterization of cocoonase gene and encoded protein obtained from A. mylitta head region. The results obtained infer the presence of cocoonase enzyme in the wild silkworm A. mylitta and can be used for cocoon degumming which will be a valuable and cost-effective strategy in silk industry.

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Section: Discussionmentioning

confidence: 99%

In silico structural and functional characterization of Antheraea mylitta cocoonase

Sneha

Pandey

2022

Journal of Genetic Engineering and Biotechnology

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“…Tasar silkworm Antheraea mylitta is basically a wild sericiginous insect, which is unique to India and it is commercially reared outdoor by tribal populace of tropical India to obtain unique silk fiber, which has great demand among the silk consumers both in India and abroad (Chakraborty et al, 2015). However, precontrived rearing system has made the tasar silkworm vulnerable to various predators and parasitoids.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of adult emergence in Xanthopimpla pedator (Fabricius)- a major pupal parasitoid of Tasar silkworm

Gadad¹,

Prabhu²,

Bhagat³

et al. 2023

JEB

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Aim: The study was conducted to elucidate the mechanism involved in adult emergence behavior of Xanthopimpla pedator a pupal parasitoid of tasar silkworm. Methodology: Behavioral observations were made to study the mechanical approaches used during emergence and SDS-PAGE analysis was carried to know the possibility of proteases in the digestive secretions. Results: The findings of the study suggest that X. pedator uses physical and chemical to achieve safe passage from the host pupae and cocoons. It was observed that during the process of emergence, X. pedator breaks host pupae by means of mandibular action (by biting the pupal shell). Further, it was noticed that to make emergence hole on host cocoon X. pedator found to employ digestive secretion to soften and disintegrate the silken shell, besides it also deploys mandibles to spread out the secretion on intended place of exit hole. SDS-PAGE analysis showed the presence of series of enzymes, possibly involved in cocoon softening. Interpretation: Findings of this study forms the basis that X. pedator, a major pupal parasitoid of tasar silkworm, uses both physical and chemical approaches to achieve safe passage from the host pupae and protective silken cocoon. Key words: Digestive secretion, Mandibular action, Parasitization, Xanthopimpla pedator

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“…Tasar silkworm seed and commercial crop rearing activities conducted in exposed conditions close to the forest fringes. Further, the wild populations also feed and breed within the surrounding forest patches (Chakraborty et al, 2015). Eco-races like raily, laria, barafand Korbiare very popular in Chhattisgarh, Daba, Sarihan, Munga, Modia, Laria, Lodhma, Palma, Japla, Kowa and Barharwa in Jharkhand, Modal, Nalia, Sukinda, Boadh, Simlipal, Omarkote and Sukly in Odisha, Andhra local in Telangana and Bhandara in Maharashtra (Mahendran et al, 2006;Saha and Kundu, 2006).…”

Section: Introductionmentioning

confidence: 99%

Spatial Variation in Cocoon Yield in Tropical Tasar Silkworm: An Influence of Insect-Predators and Pathogens

Chandrashekharaiah¹,

Gv²,

Ms³

et al. 2022

PLANT ARCHIVES

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Tropical tasar silkworm is a polyphagous silk spinning insect, endemic to India. The silkworms are commercially reared on field-raised host plants, which are geographically contiguous with the wild silkworm fauna. Ecologically, the silkworm is r-selected and evolved with complex survival strategies. Naturally, the population regulated by the interactions between host and natural enemies and their dynamics due to tri -trophic interactions. Hence, understanding the effect of biotic and abiotic factors on silkworm is most critical for increasing the productivity of the cocoons. The cocoon yield trends at different locations are an indication for local specific problems and requirements for specific technological interventions. Cocoon yield obtained at different locations during 2015 to 2017 was analysed for variation in cocoon yield. Further, predator dynamics and incidence of silkworm diseases were also measured to know their impact on silkworms. The results revealed that the highest cocoon yield was recorded in Chhattisgarh (59.97) and West Bengal (54.57) and lowest was in Maharashtra (34.92) and Madhya Pradesh (37.80). Average cocoon yield in Jharkhand and Odisha was 40.18 and 43.30, respectively. Based on the tukeys HSD test, the highest cocoon yield per dfl was recorded at Bilaspur and Pali. The next highest was recorded in Sundergarh, Boirdadar, Patelnaga and Bastar. The significant changes in the predator's population were recorded before and after brushing of the silkworm. Highest incidence was recorded during 1 st , 2 nd and 3 rd instar silkworm and reduced in the subsequent stages. Correlation studies indicated that there was a strong positive linear relationship between silkworm mortality and predator population. Similarly, the silkworm mortality due to disease-causing pathogens was 53.70 %, 26.75 % and 10.90 % during the first crop and 29.27%, 12.10 % and 9.70 % during the second crop in Jharkhand, Madhya Pradesh and Chhattisgarh, respectively. The study showed that the spatial variation in cocoon yield wasinfluenced by the sporadic occurrence of insect-predators and disease-causing organisms at critical stages of rearing.

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Genetic analysis of Indian tasar silkmoth (Antheraea mylitta) populations

Cited by 18 publications

References 96 publications

In silico structural and functional characterization of Antheraea mylitta cocoonase

In silico structural and functional characterization of Antheraea mylitta cocoonase

Mechanism of adult emergence in Xanthopimpla pedator (Fabricius)- a major pupal parasitoid of Tasar silkworm

Spatial Variation in Cocoon Yield in Tropical Tasar Silkworm: An Influence of Insect-Predators and Pathogens

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