Genetic analysis of ion-beam induced extremely late heading mutants in rice

Abstract

Two extremely late heading mutants were induced by ion beam irradiation in rice cultivar ‘Taichung 65’: KGM26 and KGM27. The F2 populations from the cross between the two mutants and Taichung 65 showed clear 3 early: 1 late segregation, suggesting control of late heading by a recessive gene. The genes identified in KGM26 and KGM27 were respectively designated as FLT1 and FLT2. The two genes were mapped using the crosses between the two mutants and an Indica cultivar ‘Kasalath’. FLT1 was located on the distal end of the short arm of chromosome 8. FLT2 was located around the centromere of chromosome 9. FLT1 might share the same locus as EHD3 because their chromosomal location is overlapping. FLT2 is inferred to be a new gene because no gene with a comparable effect to that of this gene was mapped near the centromere of chromosome 9. In crosses with Kasalath, homozygotes of late heading mutant genes showed a large variation of days to heading, suggesting that other genes affected late heading mutant genes.

Distribution of <i>Hwc2-1</i>, a causal gene of a hybrid weakness, in the World Rice Core collection and the Japanese Rice mini Core collection: its implications for varietal differentiation and artificial selection

“…Our preliminary analysis revealed that these DNA markers have more than four alleles, which were difficult to distinguish from each other. Therefore, we used a closely linked Indel marker KGC9M8 ( Ichitani et al 2014 ) instead of E1828. The 40 DNA Indel markers in Supplemental Table 1 were used for this study.…”

Drought Tolerance and Evaluation of Genetic Changes in Rice Mutant Lines

“…Mutation induction by gamma rays have been contributed for improving of crops (Ichitani et al, 2014;Parry et al, 2009;Oladosu et al, 2016;Mustikarini et al, 2016), this technique has played an influential role in increasing the world food security. Since new food crop varieties embedded with various induced mutations have contributed to the significant increase of crop production.…”

Identification of Heading Date Six (Hd6) Gene Derived from Rice Mutant Varieties

“…Induction mutation by gamma rays was successfully used for generating novel characteristics of direct importance in rice production and of potential quality and nutritional value (Ichitani et al, 2014;Parry et al, 2009;Oladosua et al, 2016). According to the data base of Atomic Energy Agency (IAEA), 3,222 officially released mutant varieties worldwide in more than 200 crop species (FAO/IAEA, 2015).…”

“…Hori et al, 2015;Lee and An 2015;Zhang et al, 2015;Zhan et al, 2015). Study on the structure of genes associated with response to photoperiod on flowering of rice has been reported by Nakamichi 2015 andYoshitake et al, 2015, which is triggered by day length (Ichitani et al, 2014) and is regulated by many genes (Itoh and Izawa 2013;Tsuji et al 2011), andEbana et al (2011) also reported as a negative control. Leaves of plants were taken from the plants grown in the greenhouse at PAIR -BATAN Jakarta in 2014.…”