Genetic analysis of liver catalase activity in two substrains of C57BL mice

Heston, W. E.; Hoffman, Harold A.; Rechcigl, Miloslav

doi:10.1017/s0016672300004274

Cited by 20 publications

(9 citation statements)

References 2 publications

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“…The analysis was performed in two steps; first, for each % suppression value the likelihood (predictive density) that a UV suppression value belonged to each genotypic group separately was computed, using the mean and standard deviation of the group; predictive densities were calculated as follows (Geisser 1964;Heston et al 1%5): E where z is the UV % suppression value, N1, RI, and Sl are the sample stze, mean, and standard deviation, respectively, for the genotypic group G,. ~Ihe predictive density for each UV suppression value was then combined with the a priori genetic probability of a segregating class to obtain the probability (posterior probability) that the UV-suppression value belonged to a specific genotypic group and segregating class; posterior probabilities were calculated as follows (Geisser 1964;Heston et al 1965): , 8~, c,) q,f (z/z,, 8,, c,) t where ql is the a priori probability of a segregation class and Eqi = 1. High and low UV % suppression cut-off values were set where the likelihood ratios of posterior probabilities were maximum for each genotypic group.…”

Section: Contact Sensitization Statisticsmentioning

confidence: 99%

Control of UVB immunosuppression in the mouse by autosomal and sex-linked genes

Noonan

Hoffman

1994

Immunogenetics

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Irradiation with UVB (290-320 nm) initiates a systemic immunosuppression detectable as suppression of contact hypersensitivity (CHS). We investigated susceptibility to UV suppression in reciprocal F1-hybrid and backcross mice derived from BALB/c (low susceptibility) and C57BL/6 (high susceptibility) inbred strains. CB6F1 male mice exhibited high susceptibility and B6CF1 male mice exhibited low susceptibility, indicating a major X-linked effect in the genetic control of UV immune suppression. Females of either F1 hybrid showed intermediate suppression, consistent with random X-inactivation. A model of monogenic X-linked control was not sufficient, and evidence for the action of two genetically unlinked autosomal genes was found in parental backcross animals. Both sexes of (BALB/c x CB6F1) mice showed a 1 high:1 low ratio of phenotypes, indicating control by a major autosomal locus, Uvs1, confirmed by propagation of the high phenotype through selective backcrossing for nine generations to BALB/c. Uvs1 was not genetically linked to 12 chromosomal markers including the pigment genes b (brown) and c (albino). Backcross animals (C57BL/6 x CB6F1) showed a significant sex difference, male mice giving a 3 high:1 low ratio of phenotypes, compatible with the action of a second autosomal locus, Uvs2, in this hybrid. The findings are compatible with a model in which high phenotype (Uvs1b/Uvs1b) is dominant when subjected to recessive epistatis by the X-chromosome locus Uvs3, or by the autosomal locus Uvs2. The finding of genetic control by interacting autosomal and X-linked genes is unique. Genetically determined high susceptibility to UV immunosuppression may be an important risk factor for UV-related human diseases.

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Section: Contact Sensitization Statisticsmentioning

confidence: 99%

Control of UVB immunosuppression in the mouse by autosomal and sex-linked genes

Noonan

Hoffman

1994

Immunogenetics

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“…Liver catalase activity in the above strains follows a similar distribution with the exception that C57BL/ An and C57BL/He exhibit higher enzyme activities than the other C57BL sublines [4].…”

Section: Methodsmentioning

confidence: 63%

“…Quantitative differences in liver catalase activity (EC 1.11.1.6) for two substrains of inbred mice were found by Heston et al [4] to be under single gene control. In that study a phenotopic range of enzyme activity was correlated with a single genotype.…”

Section: Introductionmentioning

confidence: 92%

Erythrocyte Catalase in Inbred Mice

Ha¹,

Rechcigl²

1971

Enzyme

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“…At the subcellular level, brain catalase-like activity is largely associated with the microperoxisomes (Gaunt and DeDuve, 1976;Novikoff and Novikoff, 1973). (4) There is considerable evidence of functionally important natural variations in both the expression and structure of Cas1 (Reimer et al, 1994); notably the B6 inbred mouse strain is a low catalase activity variant (Heston et al, 1965). At least for liver catalase, Ganshow and Schimke (1969) concluded that the difference in catalase activity between the B6 and C3H strain was not the result of a difference in gene expression.…”

Section: Discussionmentioning

confidence: 99%

Gene Coding Variant in Cas1 Between the C57BL/6J and DBA/2J Inbred Mouse Strains: Linkage to a QTL for Ethanol‐Induced Locomotor Activation

Demarest

Hitzemann³

et al. 2002

Alcoholism Clin & Exp Res

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Background: Among some (e.g., DBA/2J or D2) but not all (C57BL/6J or B6) inbred strains of mice, ethanol has a marked psychostimulant effect. Intercrosses formed from the D2 and B6 strains have been used to detect quantitative trait loci (QTLs) for this phenotype. The major QTL is found at the mid-region of chromosome 2 (Demarest et al., 1999). This QTL has also been detected in heterogeneous stock mice (Demarest et al., 2001). A potential candidate gene in this region is Cas1, which codes for catalase. The current studies were conducted to determine (a) if there was difference in the open reading frame (ORF) of Cas1 between the D2 and B6 strains; (b) if a difference was found, was it likely that the difference had functional effects; and (c) if it could be established that Cas1 meets the criteria for QTL to gene.Methods: The open reading frame (ORF) of Cas1 was sequenced in both the D2 and B6 mouse strains. A single polymorphism was found between the strains (see below); the strain distribution pattern for this polymorphism was determined in the 36 strains of the B6XD2 (BXD) recombinant inbred (RI) series. These data were used to map the position of Cas1 as described by Cudmore et al. (1999).Results: The only difference between the D2 and B6 strains in the coding region was found at #349, G-ϾA. This will result in a difference in the amino acid sequence between the strains at amino acid #117-alanine is found in the D2 strain while threonine is found in the B6 strain. The RI strain distribution pattern for this polymorphism was used to determine the relative placement of Cas1. The estimate suggests that Cas1 is flanked by D2Mit12 and D2Mit43 and relative to D2Mit94 (which was set at 47 cM), Cas1 is located at approximately 57 cM, confirming previous estimates (see www.jax.org).Conclusions: Pharmacological data (Correa et al., 2001) strongly support the idea that Cas1 meets the criteria for QTL to gene. However, based on the mapping data, Cas1 is clearly not included in the QTL for heterogeneous stock mice. Finally, other genetic data suggest that the polymorphism is not sufficient to generate the QTL.

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Genetic analysis of liver catalase activity in two substrains of C57BL mice

Cited by 20 publications

References 2 publications

Control of UVB immunosuppression in the mouse by autosomal and sex-linked genes

Control of UVB immunosuppression in the mouse by autosomal and sex-linked genes

Erythrocyte Catalase in Inbred Mice

Gene Coding Variant in Cas1 Between the C57BL/6J and DBA/2J Inbred Mouse Strains: Linkage to a QTL for Ethanol‐Induced Locomotor Activation

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