Genetic analysis of NaCl tolerance in tomato

Abstract

ABSTRACT. We attempted to find the suitable parents for the development of tomato hybrids for high salt soils by exploiting combining ability, gene action and heterosis. Six salt-tolerant and three salt-intolerant genotypes, along with their 18 F1 crosses, were evaluated at seedling stage under 10 and 15 dS/m (NaCl) salinity stress, compared to the control level of salinity. The experiment was laid out based on a two-way complete randomized design factorial arrangement with two replications; data on root and shoot length, fresh and dry weights, leaf /Na + concentrations were recorded. There was significant variation within genotypes, lines, testers, crosses, and line × tester interaction for all plant characters studied under normal and two salinity levels. Estimates of combining ability indicated that under low (10 dS/m) and high (15 dS/m) salinities, line BL1176 and tester LO2875 showed significant GCA effects for most of the traits studied. The cross-combinations 6233 × LO2875, CLN2498A x LO2875 and BL1176 × 17902 showed highest SCA values for most of the characters under 10 and 15 dS/m, respectively. Potence ratio showed that under low and high salinities, all the traits showed over dominant type of gene action except leaf area and K + concentration (in 10 dS/m) and shoot length, and leaf area (in 15 dS/m). The highest heterosis for most of the parameters was observed in cross-combinations BL1176 × LO2875 and CLN2498A x LO2875.

Expression of an alfalfa (Medicago sativa L.) peroxidase gene in transgenic Arabidopsis thaliana enhances resistance to NaCl and H2O2

“…The chlorophyll content of transgenic leaves was significantly higher than that of WT leaves, suggesting that MsPOD increased the H 2 O 2 tolerance of the transgenic plants. Many genes that are involved in NaCl tolerance have been identified, and breeders have investigated the genetic mechanisms involved (Saeed et al, 2011). We also found that MsPOD is involved in NaCl resistance.…”

Genes with a Combination of Over-Dominant and Epistatic Effects Underlie Heterosis in Growth of Saccharomyces cerevisiae at High Temperature

“…This idea was indirectly supported also by our previous phenotypic analyses of this hybrid collection (Shapira et al, 2014 ). So far, over-dominant genes identified in previous studies had a major effect on the hybrid's phenotype (Wallace, 1957 ; Falk, 1961 ; Muller and Falk, 1961 ; Hua et al, 2003 ; Krieger et al, 2010 ; Saeed et al, 2011 ; Li et al, 2013 ). The over-dominant loci we mapped, including AEP3, had a minor effect in the SK1xS1001 background.…”

“…While many studies mapped loci contributing to heterosis, identifying over-dominant loci turned out to be a more difficult task. Consequently, there are only a few known cases, mostly in plants, of single genes that confer heterotic effect by an over-dominant interaction between their alleles (Hua et al, 2003 ; Krieger et al, 2010 ; Saeed et al, 2011 ; Li et al, 2013 ). Over-dominant loci are interesting because of the nature of interaction between their alleles that confers a synergistic effect.…”

Sodium Chloride Tolerance during Germination and Seedling Stages of Tomato (Solanum lycopersicum L.) Lines Native to Mexico

“…This corresponds to adding 10.227 g of NaCl to a liter of nutritive solution. The selection of this NaCl concentration was based on previous tests and the results from Bogoutdinova et al [5], Sanjuan-Lara et al [14], Ávila-Amador et al [37], Wafa'a [41], and Saeed et al [42]. The floating raft system used with this concentration allowed the survival of seedlings for 12 days after the application of treatments used to discriminate tolerant and susceptible genotypes.…”