Genetic Analysis of O-H Variation in &lt;i&gt;Salmonella&lt;/i&gt;

Iino, Tetsuo

doi:10.1266/jjg.36.268

Cited by 24 publications

(19 citation statements)

References 12 publications

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“…Mutation at another locus, ah I, closely linked to (or perhaps a part of) HI prevents the formation of phase-1 flagella-so that ah Imutants alternate between a normal, flagellate, phase 2 and a phase I in which the bacteria are non-flagellate, and therefore non-motile (Iino,I 96 I). Another kind of mutation, within HI or very closely linked to it, produces the ' phase-I-curly ' phenotype (Iino, 1962). Such mutants when in phase 2 produce normal flagella and are normally motile; but when in phase I they make functionally deficient flagella with an abnormally short wavelength (' curly ').…”

Section: (Accepted For Publication 30 May 1967) S U M M a R Ymentioning

confidence: 99%

“…The ah I -strains used were two of the three non-identical, non-complementing ahr-strains described by Iino (1961). Strain ~~8 7 0 is an auxotrophic derivative of Iino's strain sw 1061, which is a spontaneous ah I -mutant of LT2; we shall indicate its mutant allele as ahI-I.…”

Section: Me?' H O D Smentioning

confidence: 99%

“…Strain ~~8 7 0 is an auxotrophic derivative of Iino's strain sw 1061, which is a spontaneous ah I -mutant of LT2; we shall indicate its mutant allele as ahI-I. sw547 is a naturally occurring ahI-strain, not derived from strain L T~ (Iino, 1961); we shall indicate its mutant allele as ah1-3.…”

Section: Me?' H O D Smentioning

confidence: 99%

“…The 'phase-I-curly ' mutant used was sw 577, a Salmonella typhirnurium strain (not derived from LT2) shown by Iino (1962) to be normally motile and with normal flagella when in phase 2, but almost non-motile and with short-wavelength flagella when in phase I . As its abnormal character probably results from mutation in the structural gene for phase-1 flagellin, we shall indicate its genotype as H I-i(curly).…”

Section: Me?' H O D Smentioning

confidence: 99%

“…This strain, like other strains of this species, is stable in phase 2. Furthermore, it carries an (unexpressed) phase-I-curly allele (Stocker, unpublished;Iino, 1962). However, the transductional clone we used though it had the phase-1 antigen a which is latent in the donor strain, SL 23, did not have its (latent) phase-I-curly character-presumably because the Hr-a(curZy) allele of the donor had recombined with the HI-i allele of the L T~ recipient to produce an HI-a (non-curly) gene.…”

Section: U B P E a R C E A N D B A D Stockermentioning

confidence: 99%

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Phase Variation of Flagellar Antigens in Salmonella: Abortive Transduction Studies

Pearce

Stocker

1967

Journal of General Microbiology

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S U M M A R YMotility was transduced by phage P22 to non-motile (Jla-, etc.) Salmonella typhimuriurn strains, and the flagellar antigens of abortive transductants inferred from the inhibition by antisera of the trails they produced in semi-solid medium. When the recipient had aflla-gene closely linked to HI (phase-I flagellar antigen locus) nearly all the Jlaf abortive transductants evoked from a recipient culture in latent phase I manifested the phase-r antigens of both recipient and donor, whereas those from a culture in latent phase 2 showed neither phase-r antigen. Thus the expression of an exogenote HI allele, like that of the chromosomal HI allele, was regulated by the phase-determinant of the recipient, at or near Hz (phase-2 flagellar antigen locus). An HI gene adjacent (cis) to ah I-( H I activator gene), in the chromosome or in the exogenote, was unexpressed in ah I+ or Jla+ abortive transductants in phase I . This suggests that ah I-mutants are HI-operatornegative mutants.When the recipient was a phase-I , and therefore non-motile, culture of an ahr-or 'phase-I-curly' mutant, lysates of phase-2 cultures, but not of phase-1 cultures of the same donor, evoked trails, attributable to H2 abortive transductants. They expressed the donor Hz allele, but not the recipient H z allele-nor the previously expressed HI allele of the 'phase-Icurly' recipient. It is inferred that a phase-determinant regulates the expression of the H z gene adjacent (cis) to it (or of which it forms part) but not that of another H2 gene in the same cell; and that it controls the expression of HI via a repressor substance, not via an inducer. The exceptional HI allele HI-1,2 determines a flagellin of antigenic character 1,2, apparently identical with that determined by a common H z allele. HI-I,Z and the common HI allele H I -b were simultaneously expressed in $a+ abortive transductants, which suggests that neither H2 flagellin nor H2 messenger RNA functions as the repressor of HI. It is proposed that an operon, comprising H2 and the structural gene for an HI repressor substance, has alternative metastable states, 'on' in phase 2 and 'off' in phase I , comparable to the wild-type state and to that of an operator-negative mutant.I N T R C) 13 U C T I 0 N Most Salmonella species have two flagellar antigens, the phase-I and phase-2 antigens, and show diphasic variation. A given bacterium manifests only one of these two antigens (and is said to be in the corresponding phase) and produces descendants most of which are in the same phase as itself, but amongst these a minority in the

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Section: (Accepted For Publication 30 May 1967) S U M M a R Ymentioning

confidence: 99%

Section: Me?' H O D Smentioning

confidence: 99%

Section: Me?' H O D Smentioning

confidence: 99%

Section: Me?' H O D Smentioning

confidence: 99%

Section: U B P E a R C E A N D B A D Stockermentioning

confidence: 99%

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