Genetic Analysis of Resistance Genes for the Rice Gall Midge in Two Rice Genotypes

Kumar, Arvind; Jain, Abhinav; Sahu, Rabindra Kumar; Shrivastava, M. N.; Nair, Suresh; Mohan, M.

doi:10.2135/cropsci2004.0406

Cited by 32 publications

(15 citation statements)

References 30 publications

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“…A simple test to detect the presence of Gm1 and Gm2 in the large pool of gall midge resistant genotypes will greatly reduce the effort required to identify new genes. Random selection of genotypes and classical allelic tests have led to the characterization of 10 resistance genes so far (Kumar et al 2005). None of these genes confers resistance against all the known gall midge biotypes.…”

Section: Discussionmentioning

confidence: 99%

“…Field and greenhouse evaluation of about 50,000 germplasm accessions has resulted in the identification of over 300 primary sources of resistance . Genetic analysis in some of these sources led to the characterization of nine dominant genes and one recessive gene conferring resistance (Kumar et al 2005). Utilizing 4-5 of these sources of resistance, over 60 gall midge resistant rice varieties have been developed and released for commercial cultivation in India since 1972 .…”

Section: Introductionmentioning

confidence: 99%

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Flanking SSR markers for allelism test for the Asian rice gall midge (Orseolia oryzae) resistance genes

et al. 2007

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Screening of rice germplasm against Asian rice gall midge, Orseolia oryzae (WoodMason), biotypes in India has led to identification of over 300 resistant rice genotypes. However, only ten resistance genes have been characterized so far. Identification of new genes through classical allelism test is tedious and time consuming. We propose to use closely linked flanking Simple Sequence Repeat (SSR) markers in allelism tests for identification of resistance genes. Of the ten known gall midge resistance genes, eight have been tagged and mapped. The Gm1 and Gm2 genes have closely linked flanking markers. Hence SSR markers RM219 and RM444, flanking the gene Gm1, and RM317, RM241 along with the SCAR marker F8, flanking the gene Gm2, were selected for this study. Tests with one set of 13 genotypes likely to carry Gm1 and another set of 17 genotypes suspected to contain Gm2 suggested the presence of the respective allele in all the 13 and 15 genotypes from these sets, respectively. Classical allelism test perfectly matched with the markers test. There were two exceptions involving amplification with RM444 in cultivar Kavya and with RM241 in genotype AE20, suggesting a single recombination which could have resulted in the mismatch. All the three markers in the genotype Bhumansan and the two flanking markers RM317 and F8 in AE20 indicated the absence of the Gm2 allele. This was validated through a classical test, revealing a segregation ratio of 15 resistant: 1 susceptible F 2 progeny of both the crosses between the Gm2 source Phalguna and these genotypes. We performed the allelism test with the markers on another set of 56 randomly selected gall midge resistant genotypes to discover possible sources of new resistance genes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Flanking SSR markers for allelism test for the Asian rice gall midge (Orseolia oryzae) resistance genes

et al. 2007

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“…However, for polymorphism analysis biotypes GMB1, GMB4 and GMB4M were used. The rice ( Oryzae sativa ) cultivars used for rearing the insects were Jaya (susceptible to GMB4) and RP2068 (resistant to GMB4) (Bentur et al ., ; Kumar et al ., ). GMB4 infestation on rice cultivar Jaya leads to the formation of a gall chamber, whereas on cultivar RP2068, a hypersensitive‐mediated (HR + ) defence response is initiated and the insects succumb to the plant defence response within 96 h of egg hatching.…”

Section: Methodsmentioning

confidence: 97%

Expression ofOrseolia oryzae nucleoside diphosphate kinase(OoNDPK) is enhanced in rice gall midge feeding on susceptible rice hosts and its over‐expression leads to salt tolerance inEscherichia coli

Sinha

Atray

Bentur

et al. 2012

Insect Molecular Biology

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The Asian rice gall midge, Orseolia oryzae, is a major dipteran pest of rice, with many known biotypes. The present investigation was initiated to understand the molecular mechanisms of infestation for developing novel integrated pest management strategies. We isolated and characterized a gene, nucleoside diphosphate kinase (OoNDPK), from the rice gall midge, encoding a protein with 169 amino acid residues and with a secretory signal sequence - an observation that assumes significance as salivary gland secretions have been implicated to play a major role in insect-plant interactions. Furthermore, up-regulation (> 18 folds) of OoNDPK was observed in the salivary glands of maggots feeding on susceptible host in contrast to those feeding on resistant host. Phylogenetic analysis revealed similarity of OoNDPK with its dipteran orthologues. 3DLigandSite analysis, of the predicted OoNDPK and its orthologues, revealed phenylalanine and tyrosine residues to be specifically present in NDPK proteins from the plant feeders. Results suggest secretion of OoNDPK into the host plant and its probable involvement in gall midge-rice interaction. Using the coleoptile cell elongation assay, we demonstrated that the recombinant OoNDPK is capable of causing elongation of rice coleoptile cells. Additionally, heterologous expression of OoNDPK in Escherichia coli increased the tolerance of these cells to salt (NaCl; up to 1 mM), hinting at the involvement of this gene in abiotic stress response as well.

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“…Use of resistant varieties has been the most feasible alternative to manage the pest. Ample sources of resistance are available in cultivated rice, Oryza sativa L. Inheritance studies have identified nine dominant and one recessive gene imparting resistance to different biotypes of Asian rice gall midge (Kumar et al 2005;Pani and Sahu 2000), and eight of them have been tagged with different molecular markers. Mohan et al(1994) identfied two RFLP markers, RG329 and RG476, linked to the gall midge resistance gene, Gm2 in the rice cv.…”

Section: Introductionmentioning

confidence: 99%

Flanking Microsatellite Markers for Breeding Varieties Against Asian Rice Gall Midge

Nanda

Mohanty

Panda

et al. 2010

Tropical Plant Biol.

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The Asian rice gall midge, Orseolia oryzae WoodMason (Cecidomyiidae: Diptera) is a serious pest of wet season rice in South and Southeast Asia. Due to internal feeding habit and presence of biotypes of the pest, the most feasible way to control is breeding varieties resistant against multiple biotypes through marker-assisted breeding (MAB). But very few versatile co-dominant markers linked to the gall midge resistance genes are available. We used a set of F 9 recombinant inbred lines (RILs) of the cross TN1/PTB10 and identified microsatellite markers for the gall midge resistance gene in cv. PTB10 on short arm of rice chromosome 8. Markers RM22550 and RM547 flank the gene at a distance of 0.9 and 1.9 cM, respectively. Amplification of the markers in gall midge resistant and susceptible cultivars showed that these markers can be successfully used in MAB for development of gall midge resistant varieties.

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Genetic Analysis of Resistance Genes for the Rice Gall Midge in Two Rice Genotypes

Cited by 32 publications

References 30 publications

Flanking SSR markers for allelism test for the Asian rice gall midge (Orseolia oryzae) resistance genes

Flanking SSR markers for allelism test for the Asian rice gall midge (Orseolia oryzae) resistance genes

Expression ofOrseolia oryzae nucleoside diphosphate kinase(OoNDPK) is enhanced in rice gall midge feeding on susceptible rice hosts and its over‐expression leads to salt tolerance inEscherichia coli

Flanking Microsatellite Markers for Breeding Varieties Against Asian Rice Gall Midge

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