Genetic analysis of resistance to late leaf spot in interspecific groundnuts

Janila, Pasupuleti; Venuprasad, R.; Rathore, Abhishek; Aruna, R.; Reddy, R. K.; Waliyar, F; Nigam, S. N.

doi:10.1007/s10681-013-0881-7

Cited by 27 publications

(10 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In post-rainy 2018, the population (620) was screened in light chamber (Controlled facility) using the detached leaf assays which is a rapid screening technique for LLS [27,28]. A completely randomized (CRD) experimental design with two replications was used.…”

Section: Screening For Lls Resistance Using Detached Leaf Assay In Light Chambermentioning

confidence: 99%

Variability and Trait Association Studies for Late Leaf Spot Resistance in a Groundnut MAGIC Population

et al. 2021

Self Cite

View full text Add to dashboard Cite

Globally, late leaf spot (LLS), a foliar fungal disease is one of the most important biotic constraint in groundnut production. Multi-Parent Advanced Generation Inter Cross (MAGIC) groundnut population was developed in a convergent crossing scheme using eight founder parents to develop a mapping population for multiple traits includes LLS. The experiments conducted in light chamber using detached leaf assay, and disease field screening nurseries at two locations (ICRISAT and ARS, Kasbe Digraj) showed significant variability for LLS resistance and component of resistance traits. Total 10 MAGIC lines with longer incubation (>11.0 days) and two MAGIC lines with longer latent period (>27 days) than the resistant parent, GPBD 4 were identified. The MAGIC lines, ICGR 171413, and ICGR 171443 with a lesion diameter of <1 mm and 4.10–5.67% of leaf area damage can be valuable sources for the alleles limiting the pathogen severity. A total of 20 MAGIC lines recorded significantly superior for disease score at 105 DAP_I (5.60–6.89) compared to resistant check, GPDB 4 (6.89). Further studies to determine the type and number of genes controlling the LLS component traits in groundnut will be useful for improvement of resistance to LLS. Genomic selection approach can be valuable in groundnut breeding to harness the minor alleles contributing to the component traits of LLS resistance.

show abstract

Section: Screening For Lls Resistance Using Detached Leaf Assay In Light Chambermentioning

confidence: 99%

Variability and Trait Association Studies for Late Leaf Spot Resistance in a Groundnut MAGIC Population

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Detached, healthy groundnut leaves rooted in ster ile sand in trays are inoculated with a concentration of 30,000 spores ml-1 for LLS and 105 urediniospores ml-1 for rust, followed by incubation in the growth chamber at 24 °C temperature and 85 % relative humidity and a 12 h light/12 h dark regime. LLS disease development is determined every 2 days from 5 to 37 days after inocu lation and observations are recorded on incubation period (days), latent period (days), lesion number, lesion diameter (mm) and leaf area damage (%) (Janila et al 2013). Rust pustules appear some 10 days after inoculation on susceptible geno types.…”

Section: Screening For Foliar Fungal Diseasesmentioning

confidence: 99%

Phenotyping for Groundnut (Arachis hypogaea L.) Improvement

Janila

Nigam

2013

Phenotyping for Plant Breeding

Self Cite

View full text Add to dashboard Cite

Groundnut (Arachis hypogaea L.) is grown world over for oil and food uses. It is a self-pollinated crop with low genetic diversity. The origin of the crop from single hybridization event followed by chromosome doubling as well as cross ing barriers of cultivated species with wild species due to ploidy differences ren dered the crop with narrow genetic variability. Developing new varieties with increased yield potential and resistance to biotic and abiotic stresses that meet the needs of the growers, processors and consumers is the primary objective of ground nut breeding. In this chapter, we discuss about phenotyping tools used in groundnut improvement programs for various targeted traits. Both field and laboratory tools are described to screen for resistance to diseases caused by fungi, bacteria, virus and nematodes. Phenotyping based on Cumulative Thermal Time (CTT) is used to select for early maturity. Phenotyping for complex traits can be challenging. Either empirical approach that involves measuring the yield under imposed drought stress or salinity conditions or trait based approach using surrogates or a combination of both are used for phenotyping abiotic stresses. Phenotyping for Aspergillus con tamination needs improvement to derive reliable and reproducible results. Estimation of quality and nutritional parameters generally involves use of destructive and labo rious chemical or physical procedures. Near infrared reflectance spectroscopy (NIRS), a robust and non-destructive method is gaining popularity for estimation of oil, protein, carbohydrate and fatty acid contents. Methods for estimating oil, pro tein, sugar and micronutrient concentrations and fatty acid composition of seeds and haulm quality traits are described.

show abstract

“…Kimber and Paull (2011) studied the genetics of resistance to Cercospora leaf spot in Feba bean and reported a monogenic dominant gene control leaf spot resistance with resistance dominant over susceptibility. Pasupuleti et al (2013) reported that late leaf spot in groundnut was governed by a combination of both, nuclear and maternal gene effects. Little or no information is available on the genetics of CLS resistance in Nigerian cowpea varieties.…”

Section: Introductionmentioning

confidence: 99%

Inheritance of resistance to Cercospora leaf spot disease of cowpea [Vigna unguiculata (L.) Walp]

et al. 2019

View full text Add to dashboard Cite

Cercospora leaf spot (CLS) caused by Pseudocercospora cruenta (Sacc.) is an important disease affecting cowpea production in Nigeria. Understanding the genetic nature of CLS is an important step in developing an effective breeding strategy. This study investigated the inheritance of CLS disease in cowpea under natural epiphytotic field condition involving two CLS resistant parents (IT99K-573-1-1, IT99K216-24) and a CLS susceptible parent (UAM09-1055-6). The parental lines, F 1 , BC 1 P 1 , BC 1 P 2 , F 2 and F 3 generations were used to study the genetic nature and to detect SSR markers closely linked with the CLS resistance gene(s) using bulked segregant analysis (BSA). The result showed that F 1 populations involving UAM09-1055-6 9 IT99K-573-1-1 and UAM09-1055-6 9 IT99K-216-24 were resistant to CLS in the 2 crosses suggesting the presence of gene dominance in the control of the disease. The observed segregating ratio of F 2 populations fits the Mendalian ratio 3:1. The plants reaction to the disease in the backcross progeny test involving the resistant parent were all uniformly resistant, whereas those involving the susceptible parent segregated into ratio 1:1. The F 3 generations, which segregated into ratio 1:2:1 further confirmed that resistance was controlled by a single dominant gene in the crosses studied. Heritability estimates varied from 81 to 97%. BSA showed that SSR marker code named RB24 of lima bean and validated on F 2 population discriminated between resistance and susceptibility to CLS. Hence RB24 could be a useful marker for marker-assisted selection in CLS resistance breeding in cowpea.

show abstract

Genetic analysis of resistance to late leaf spot in interspecific groundnuts

Cited by 27 publications

References 39 publications

Variability and Trait Association Studies for Late Leaf Spot Resistance in a Groundnut MAGIC Population

Variability and Trait Association Studies for Late Leaf Spot Resistance in a Groundnut MAGIC Population

Phenotyping for Groundnut (Arachis hypogaea L.) Improvement

Inheritance of resistance to Cercospora leaf spot disease of cowpea [Vigna unguiculata (L.) Walp]

Contact Info

Product

Resources

About