Genetic analysis of RNA1 and RNA2 of Macrobrachium rosenbergii nodavirus (MrNV) isolated from India

Kumar, Sekar Ashok; Shekar, Malathi; Karunasagar, Iddya; Karunasagar, Indrani

doi:10.1016/j.virusres.2013.01.003

Cited by 51 publications

(43 citation statements)

References 38 publications

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“…This observation would have explained the inability to cluster MrNV and PvNV in neither alpha‐ nor betanodavirus due to the distinct amino acid sequence encoded by the significant different nucleotide preference and composition of its capsid. Current findings are in agreement to previous reports on the diversity in capsid topologies among the members of the Nodaviridae (Chen et al, ; Low, Md Yusoff, Kuppusamy, & Ahmad Nadzri, ; Odegard, Banerjee, & Johnson, ), where sequence similarity of both MrNV and PvNV is not more than 20% with that alpha‐ and betanodavirus, whilst the capsid protein sequence similarity is more than 80% between MrNV and PvNV (Chong, Ganesan, Yong, Tan, & Ho, ; NaveenKumar et al, ). This may lead to an assumption of nodavirus capsid evolution in a non‐linear form within the family group (Warrilow et al, ).…”

Section: Discussionsupporting

confidence: 92%

Comparative genome analysis reveals a distinct influence of nucleotide composition on virus–host species‐specific interaction of prawn‐infecting nodavirus

Ismail

Baharum

Fazry

et al. 2019

Journal of Fish Diseases

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Discovery of species‐specific interaction between the host and virus has drawn the interest of many researchers to study the evolution of the newly emerged virus. Comparative genome analysis provides insights of the virus functional genome evolution and the underlying mechanisms of virus–host interactions. The analysis of nucleotide composition signified the evolution of nodavirus towards host specialization in a host‐specific mutation manner. GC‐rich genome of betanodavirus was significantly deficient in UpA and UpU dinucleotides composition, whilst the AU‐rich genome of gammanodavirus was deficient in CpG dinucleotide. The capsid of MrNV and PvNV of gammanodavirus retains the highest abundance of adenine and uracil at the second codon position, respectively, which were found to be very distinctive from the other genera. ENC‐GC3 plot inferred the influence of natural selection and mutational pressure in shaping the evolution of MrNV RdRp and capsid, respectively. Furthermore, CAI/eCAI analysis predicts a comparable adaptability of MrNV in squid, Sepia officinalis than its natural host, Macrobrachium rosenbergii. Thus, further study is warranted to investigate the capacity of MrNV replication in S. officinalis owing to its high codon adaptation index.

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Section: Discussionsupporting

confidence: 92%

Comparative genome analysis reveals a distinct influence of nucleotide composition on virus–host species‐specific interaction of prawn‐infecting nodavirus

Ismail

Baharum

Fazry

et al. 2019

Journal of Fish Diseases

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“…6 for a review]. Based on the amino acid sequence of the RNA dependent RNA polymerase (RdRp), Mr NV is grouped within the Alphanodavirus genus but owing to some unique characteristics of Mr NV, a new genus ( Gammanodavirus ) has been proposed which would comprise Mr NV and Penaeus vannamei nodavirus ( Pv NV) 9 . In common with other nodaviruses, Mr NV is a non-enveloped, icosahedral virus which comprises a nucleocapsid containing a bipartite positive-sense RNA genome.…”

Section: Introductionmentioning

confidence: 99%

Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution

Kueh

Beh

et al. 2017

Sci Rep

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White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. There are no interventions available to treat or prevent MrNV disease however. Here we show the structure of MrNV virus-like particles (VLPs) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T = 3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that MrNV may belong to a new genus of the Nodaviridae. Moreover, our study provides the first structural view of an important pathogen affecting aquaculture industries across the world.

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“…Mr NV belongs to the family Nodaviridae which generally contains two genera: the Alpha‐nodavirus that infects insects, and the Beta‐nodavirus that infects fishes. Mr NV has been proposed to be grouped into a new genus, the Gamma‐nodavirus which infects prawns . Prawn nodavirus includes Mr NV and Penaeus vannamei nodavirus ( Pv NV).…”

Section: Introductionmentioning

confidence: 99%

Virus‐like particle of Macrobrachium rosenbergii nodavirus produced in Spodoptera frugiperda (Sf9) cells is distinctive from that produced in Escherichia coli

Kueh

Yong

Dezfooli

et al. 2016

Biotechnology Progress

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Macrobrachium rosenbergii nodavirus (MrNV) is a virus native to giant freshwater prawn. Recombinant MrNV capsid protein has been produced in Escherichia coli, which self-assembled into virus-like particles (VLPs). However, this recombinant protein is unstable, degrading and forming heterogenous VLPs. In this study, MrNV capsid protein was produced in insect Spodoptera frugiperda (Sf9) cells through a baculovirus system. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) revealed that the recombinant protein produced by the insect cells self-assembled into highly stable, homogenous VLPs each of approximately 40 nm in diameter. Enzyme-linked immunosorbent assay (ELISA) showed that the VLPs produced in Sf9 cells were highly antigenic and comparable to those produced in E. coli. In addition, the Sf9 produced VLPs were highly stable across a wide pH range (2-12). Interestingly, the Sf9 produced VLPs contained DNA of approximately 48 kilo base pairs and RNA molecules. This study is the first report on the production and characterization of MrNV VLPs produced in a eukaryotic system. The MrNV VLPs produced in Sf9 cells were about 10 nm bigger and had a uniform morphology compared with the VLPs produced in E. coli. The insect cell production system provides a good source of MrNV VLPs for structural and immunological studies as well as for host-pathogen interaction studies. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:549-557, 2017.

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Genetic analysis of RNA1 and RNA2 of Macrobrachium rosenbergii nodavirus (MrNV) isolated from India

Cited by 51 publications

References 38 publications

Comparative genome analysis reveals a distinct influence of nucleotide composition on virus–host species‐specific interaction of prawn‐infecting nodavirus

Comparative genome analysis reveals a distinct influence of nucleotide composition on virus–host species‐specific interaction of prawn‐infecting nodavirus

Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution

Virus‐like particle of Macrobrachium rosenbergii nodavirus produced in Spodoptera frugiperda (Sf9) cells is distinctive from that produced in Escherichia coli

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