Genetic analysis of salt tolerance in a progeny derived from the citrus rootstocks Cleopatra mandarin and trifoliate orange

Raga, V.; Intrigliolo, Diego S.; Bernet, G. P.; Carbonell, E. A.; Asíns, M. J.

doi:10.1007/s11295-016-0991-1

Cited by 25 publications

(19 citation statements)

References 52 publications

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“…This is the first report on identification of QTLs related to HLB disease and tolerance. Most of the recently reported QTLs in citrus are related to morphological and physiological traits ( Kepiro and Roose, 2010 ; Sugiyama et al, 2011 ; Sahin-Cevik and Moore, 2012 ; Raga et al, 2014 ; Asins et al, 2015 ; Raga et al, 2016 ; Yu et al, 2016 ; Curtolo et al, 2017 ; Imai et al, 2017 ; Yu et al, 2017 ). Only a few reports focused on disease-related QTLs in citrus, such as resistance to citrus tristeza virus (CTV) ( Asins et al, 2012 ; Ohta et al, 2015 ), Alternaria brown spot (ABS) ( Cuenca et al, 2013 , 2016 ), citrus leprosis virus (CiLV) ( Bastianel et al, 2009 ), and citrus nematode ( Ling et al, 2000 ).…”

Section: Discussionmentioning

confidence: 99%

Construction of High-Density Genetic Maps and Detection of QTLs Associated With Huanglongbing Tolerance in Citrus

Huang

Roose

et al. 2018

Front. Plant Sci.

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Huanglongbing (HLB), or citrus greening, is the most devastating disease in citrus worldwide. Commercial citrus varieties including sweet orange (Citrus sinensis) are highly susceptible to HLB, and trifoliate orange (Poncirus trifoliata, a close Citrus relative) is widely considered resistant or highly tolerant to HLB. In this study, an intergeneric F1 population of sweet orange and trifoliate orange was genotyped by Genotyping-by-Sequencing, and high-density SNP-based genetic maps were constructed separately for trifoliate orange and sweet orange. The two genetic maps exhibited high synteny and high coverage of the citrus genome. Progenies of the F1 population and their parents were planted in a replicated field trial, exposed to intense HLB pressure for 3 years, and then evaluated for susceptibility to HLB over 2 years. The F1 population exhibited a wide range in severity of HLB foliar symptom and canopy damage. Genome-wide QTL analysis based on the phenotypic data of foliar symptom and canopy damage in 2 years identified three clusters of repeatable QTLs in trifoliate orange linkage groups LG-t6, LG-t8 and LG-t9. Co-localization of QTLs for two traits was observed within all three regions. Additionally, one cluster of QTLs in sweet orange (linkage group LG-s7) was also detected. The majority of the identified QTLs each explained 18–30% of the phenotypic variation, indicating their major role in determining HLB responses. These results show, for the first time, a quantitative genetic nature yet the presence of major loci for the HLB tolerance in trifoliate orange. The results suggest that sweet orange also contains useful genetic factor(s) for improving HLB tolerance in commercial citrus varieties. Findings from this study should be very valuable and timely to researchers worldwide as they are hastily searching for genetic solutions to the devastating HLB crisis through breeding, genetic engineering, or genome editing.

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Section: Discussionmentioning

confidence: 99%

Construction of High-Density Genetic Maps and Detection of QTLs Associated With Huanglongbing Tolerance in Citrus

Huang

Roose

et al. 2018

Front. Plant Sci.

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“…This is the first report on identification of QTLs related to C Las infection and HLB tolerance. Most of the recently reported QTLs in citrus are related to morphological and physiological traits (Asins et al , 2015; Curtolo et al , 2017; Imai et al , 2017; Kepiro and Roose, 2010; Raga et al , 2014; Raga et al , 2016; Sahin-Cevik and Moore, 2012; Sugiyama et al , 2011; Yu et al , 2017; Yu et al , 2016). Only a few reports focused on disease-related QTLs in citrus, such as Citrus tristeza virus (CTV) (Asins et al , 2012; Ohta et al , 2015), Alternaria brown spot (ABS) (Cuenca et al , 2016; Cuenca et al , 2013), and Citrus leprosis virus (CiLV) (Bastianel et al , 2009).…”

Section: Discussionmentioning

confidence: 99%

Construction of high-density genetic maps and detection of QTLs associated with Huanglongbing infection in citrus

Huang

Roose

et al. 2018

Preprint

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No true resistance to Huanglongbing (HLB), a citrus disease associated with infection of Candidatus Liberibacter asiaticus (CLas), is found within commercial citrus cultivars, though trifoliate orange (Poncirus trifoliata) has been described as resistant or tolerant. Through genotyping an intergeneric F1 population by Genotyping-by-Sequencing, high-density SNP-based genetic maps were constructed separately for trifoliate orange and sweet orange (Citrus sinensis). Both genetic maps exhibited high synteny and high coverage of citrus genome. After exposure to intense HLB pressure for two years, Ct value of qPCR for CLas detection in leaves throughout ten time points during the next three years was above 35 in trifoliate oranges, under 28 in sweet oranges, and ranged from 24 to 38 and exhibited obvious segregation among progenies. Phenotypic data of percentage of healthy trees showed high correlation with the Ct value. By mapping the two traits at all time points, a total of nine clusters of QTLs were detected, of which five, respectively located on LG-t7 and LG-t8 of trifoliate orange map and LG-s3, LG-s5 and LG-s9 of sweet orange map, collectively explained a major part of the phenotypic variation. This study provides a starting point for citrus breeding to support long-term control of this devastating disease.Highlight1). Constructed the first high-density genetic map for trifoliate orange (Poncirus trifoliata)2). The first report on identification of QTLs related to Huanglongbing in citrus.AbbreviationsACPAsian citrus psyllidCLasCandidatus Liberibacter asiaticuscMcentiMorgansCtCycle thresholdHLBHuanglongbingIMInterval mappingKWKruskal-WallisLGLinkage groupLODLogarithm of oddsQTLQuantitative trait locusRADRestriction site associated DNArMQMrestricted multiple QTL mappingSNPSingle nucleotide polymorphism.

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“…Unfortunately, QTL and bulk segregant analyses for the identification of markers linked with tolerance to abiotic stresses still remain limited (Weber et al, 2003; Ben-Hayyim and Moore, 2007; Raga et al, 2016).…”

Section: Selection Of Improved Cultivarsmentioning

confidence: 99%

Facing Climate Change: Biotechnology of Iconic Mediterranean Woody Crops

et al. 2019

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The Mediterranean basin is especially sensitive to the adverse outcomes of climate change and especially to variations in rainfall patterns and the incidence of extremely high temperatures. These two concurring adverse environmental conditions will surely have a detrimental effect on crop performance and productivity that will be particularly severe on woody crops such as citrus, olive and grapevine that define the backbone of traditional Mediterranean agriculture. These woody species have been traditionally selected for traits such as improved fruit yield and quality or alteration in harvesting periods, leaving out traits related to plant field performance. This is currently a crucial aspect due to the progressive and imminent effects of global climate change. Although complete genome sequence exists for sweet orange ( Citrus sinensis ) and clementine ( Citrus clementina ), olive tree ( Olea europaea ) and grapevine ( Vitis vinifera ), the development of biotechnological tools to improve stress tolerance still relies on the study of the available genetic resources including interspecific hybrids, naturally occurring (or induced) polyploids and wild relatives under field conditions. To this respect, post-genomic era studies including transcriptomics, metabolomics and proteomics provide a wide and unbiased view of plant physiology and biochemistry under adverse environmental conditions that, along with high-throughput phenotyping, could contribute to the characterization of plant genotypes exhibiting physiological and/or genetic traits that are correlated to abiotic stress tolerance. The ultimate goal of precision agriculture is to improve crop productivity, in terms of yield and quality, making a sustainable use of land and water resources under adverse environmental conditions using all available biotechnological tools and high-throughput phenotyping. This review focuses on the current state-of-the-art of biotechnological tools such as high throughput –omics and phenotyping on grapevine, citrus and olive and their contribution to plant breeding programs.

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Genetic analysis of salt tolerance in a progeny derived from the citrus rootstocks Cleopatra mandarin and trifoliate orange

Cited by 25 publications

References 52 publications

Construction of High-Density Genetic Maps and Detection of QTLs Associated With Huanglongbing Tolerance in Citrus

Construction of High-Density Genetic Maps and Detection of QTLs Associated With Huanglongbing Tolerance in Citrus

Construction of high-density genetic maps and detection of QTLs associated with Huanglongbing infection in citrus

Facing Climate Change: Biotechnology of Iconic Mediterranean Woody Crops

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