Genetic Analysis of SNPs in GH, GHR, IGF-I and IGFBPII Genes and their Association with Some Productive and Reproductive Traits in Native Breeder Hens

Abstract

The aim was to study the polymorphisms in GH, GHR, IGF-I and IGFBPII genes and their association with productive and reproductive traits. DNA was extracted from blood samples of 380 breeder hens of Mazandaran native fowls breeding station by modified salting out method. For genotyping, PCR products of GH, GHR, IGF-I and IGFBPII loci were digested with specific restriction enzymes. We found six genotypes of AA, BB, CC, AB, AC and BC with the frequency of 0.10, 0.01, 0.36, 0.07, 0.34 and 0.12 in GH, two haploid alleles and genotypes of B+ and B-with the frequency of 0.72 and 0.28 in GHR, three genotypes of BB, Bb, bb with the frequency of 0.49, 0.44 and 0.07 in IGF-I and three genotypes of CC, CT and TT with the frequency of 0.14, 0.62 and 0.24 in IGFBPII loci, respectively. Significant associations were found between SNPs in IGF-I locus and egg number at 120-270 and 345-375 days of age, egg weight at puberty and percentage of hatchability traits (P<0.05). We also found significant effects of GHR and IGFBPII loci on egg number at 345-375 days of age and average egg weight at 345-375 days of age traits, respectively (P<0.05). Mean comparisons analysis between different genotypes of GH gene for body weight at day one and puberty, age at sexual maturity, egg weight at puberty and 30 weeks of age, average egg weight and egg number at 345-375 days of age and also percentage of fertility traits and IGFBPII genotypes for laying intensity trait were observed to be significant (P<0.05). Regarding the significant effects of GHR, IGF-I and IGFBPII genes on egg production traits, it could be resulted that these candidate genes may be used as the candidate markers in poultry breeding programs to improve productive and reproductive traits in this population.

Effects of dietary supplementation of herbal active ingredients promoting insulin-like growth factor-1 secretion on production performance, egg quality, blood hematology, and excreta gas emission in laying hens

“…A high circulating IGF-1 concentration in laying hens is related to a high egg weight [16], egg production rate [17], and feed efficiency [18]. Liu et al [19] reported that the increase of serum IGF-1 concentration in geese corresponded to the improvement of egg weight and egg production rate and the reduction of feed conversion ratio.…”

“…Yang et al [ 15 ] noted that the production performance improved in laying hens fed the diet supplemented with herbal active ingredients was due to the enhancement of serum IGF-1 concentration. A high circulating IGF-1 concentration in laying hens is related to a high egg weight [ 16 ], egg production rate [ 17 ], and feed efficiency [ 18 ]. Liu et al [ 19 ] reported that the increase of serum IGF-1 concentration in geese corresponded to the improvement of egg weight and egg production rate and the reduction of FCR.…”

Single Nucleotide Polymorphisms of Candidate Genes Related to Egg Production Traits in Vietnamese Indigenous Chickens

“…Thus, this gene consequently result in three genotypes, AA (620 and 280 bp), BB (450, 280 and 170 bp) and AB (620, 450, 280 and 170 bp). The results show that the DNA banding patterns of GH was not respectively consistent with what was previously reported byTanaka et al (1992);Stephen et al (2001); orKazemi et al (2018). When studying native chicken of Iran,Kazemi et al (2018) show a PCR fragments of 776 bp; and using restriction reaction, with MspI restriction enzyme, detected polymorphism in intron 1 of GH gene.…”

“…The results show that the DNA banding patterns of GH was not respectively consistent with what was previously reported byTanaka et al (1992);Stephen et al (2001); orKazemi et al (2018). When studying native chicken of Iran,Kazemi et al (2018) show a PCR fragments of 776 bp; and using restriction reaction, with MspI restriction enzyme, detected polymorphism in intron 1 of GH gene. The result obtained three kind of alleles of A, B and C and 6 different haplotype profiles (AA, BB, CC, AB, AC and BC).…”

Functional genes polymorphism associations with egg quality traits in the populations of dual-purpose

“…A similar situation was found for insulin-like growth factor I gene. In general, its functioning is directly associated with GH and GHR, so they may be viewed as a unifi ed functional system, which is more than justifi ed from a physiological standpoint (Attarchi et al, 2017;Kazemi et al, 2018). However, regardless of perspectives, the phenomenon of potential breed specifi city imposes limitations -it is not always possible to determine the association between different alleles of IGF-I and productive traits.…”