Genetic analysis of the 2019 coronavirus pandemic with from real-time reverse transcriptase polymerase chain reaction

Farasani, Abdullah

doi:10.1016/j.sjbs.2020.11.035

Cited by 10 publications

(4 citation statements)

References 43 publications

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“…Currently, quantitative real-time polymerase chain reaction (qRT-PCR) measurement of the SARS-CoV-2 nucleic acid is an important method for the diagnosis of COVID-19 in China to determine whether patients can be discharged from the hospital and/or need to enter isolation. 6 However, a negative qRT-PCR test result is not sufficient to exclude SARS-CoV-2 infection, and a combination of symptoms, radiological examination, and hematological examination are required to improve the sensitivity and accuracy of COVID-19 diagnosis. 7 Published studies have revealed that some hematological/biochemical changes in patients (e.g., normal or decreased leukocytes) may contribute to the diagnosis of COVID-19.…”

mentioning

confidence: 99%

Genome‐wide screening of SARS‐CoV‐2 infection‐related genes based on the blood leukocytes sequencing data set of patients with COVID‐19

Gào

Liu

Zou

et al. 2021

Journal of Medical Virology

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Coronavirus disease 2019 (COVID‐19) is a global epidemic disease caused by a novel virus, severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), causing serious adverse effects on human health. In this study, we obtained a blood leukocytes sequencing data set of COVID‐19 patients from the GEO database and obtained differentially expressed genes (DEGs). We further analyzed these DEGs by protein–protein interaction analysis and Gene Ontology enrichment analysis and identified the DEGs closely related to SARS‐CoV‐2 infection. Then, we constructed a six‐gene model (comprising IFIT3, OASL, USP18, XAF1, IFI27 , and EPSTI1 ) by logistic regression analysis and calculated the area under the ROC curve (AUC) for the diagnosis of COVID‐19. The AUC values of the training group, testing group, and entire group were 0.930, 0.914, and 0.921, respectively. The six genes were highly expressed in patients with COVID‐19 and positively correlated with the expression of SARS‐CoV‐2 invasion‐related genes ( ACE2, TMPRSS2, CTSB , and CTSL ). The risk score calculated by this model was also positively correlated with the expression of TMPRSS2, CTSB , and CTSL , indicating that the six genes were closely related to SARS‐CoV‐2 infection. In conclusion, we comprehensively analyzed the functions of DEGs in the blood leukocytes of patients with COVID‐19 and constructed a six‐gene model that may contribute to the development of new diagnostic and therapeutic ideas for COVID‐19. Moreover, these six genes may be therapeutic targets for COVID‐19.

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mentioning

confidence: 99%

Genome‐wide screening of SARS‐CoV‐2 infection‐related genes based on the blood leukocytes sequencing data set of patients with COVID‐19

Gào

Liu

Zou

et al. 2021

Journal of Medical Virology

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“…Two basic approaches exist in the immunochemical diagnoses of COVID-19: fi rstly, detection of the antibodies specifi c to SARS-CoV-2 produced as the results of the disease and, sec-ondly, detection of the SARS-CoV-2 antigen in an immunoassay (37). Besides the immunochemical tests, genetic methods play a signifi cant role (38)(39)(40).…”

Section: The Common Antigen Tests and Comparison With The Other Methodsmentioning

confidence: 99%

Point-of-care diagnosis of COVID-19 disease based on antigen tests

Pohanka¹

2021

BLL

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AIMS: This review is focused on the laboratory diagnoses of the coronavirus disease 2019 (COVID-19) by recognizing the antigen of the causative agent SARS-CoV-2 virus. Various antigen tests are available in this moment and these tests are being further developed in order to reach a better diagnostic value. The issue is reviewed in a complex view. METHODS: In this work, a complex survey of the current literature was made. The relevant and recent papers related to antigen tests of COVID-19 are discussed and cited. Basic specifi cations of the antigen tests and competitive methods were also scrutinized in the current literature. RESULTS: The survey of the current literature (years 2019 -2021) was made and diagnostic methods like lateral fl ow tests (lateral fl ow immunochromatographic assay) and various types of biosensors were specifi ed as tools for COVID-19 diagnosis and their application to be used as a point-of-care test is considered. CONCLUSIONS: Small hand-held assays applicable in the point-of-care conditions for diagnosis of COVID-19 by analysis of SARS-CoV-2 antigen are the means of a growing interest and these means undergo a signifi cant development leading to the improvements of their specifi cations and applicability to the current praxis. Merit of the assays is discussed in this paper (Tab. 3, Fig. 2, Ref. 109).

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“…A variety of clinical diseases, such as arboviruses, COVID-19, chlamydia trachomatis, cytomegalovirus, hepatitis B Virus, hepatitis C virus, tuberculosis, human papilloma virus, enterovirus, sexually transmitted infections, and bacterial infections, could be detected using these assays to detect infectious agents and nucleic acid [28] , [41] , [44] , [45] . Due to the pandemic of COVID-19 infectious disease, PCR, namely RT-PCR, is now well-known in the year 2020 [13] .…”

Section: Diagnosismentioning

confidence: 99%