Genetic analysis of the pX region of bovine leukemia virus genotype 1 in Holstein Friesian cattle with different stages of infection

Machuca, Neli Montero; Pérez, Jorge Luis Tórtora; Méndez, Ana Silvia González; García-Camacho, Angélica Lucia; Flamand, Ernesto Marín; Álvarez, Hernández

doi:10.1007/s00705-021-05252-2

Cited by 5 publications

(4 citation statements)

References 56 publications

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“…Finally, our sequences allow us to confirm the presence of the BLV genotype-1 in Creole breeds from Colombia. Genotype 1 is distributed worldwide ( Polat et al, 2017 ), and has been found in different regions of the Americas, including Brazil, Uruguay, Argentina, Peru, and Mexico ( Camargos et al, 2002 ; Montero Machuca et al, 2022 ; Moratorio et al, 2010 ; Polat et al, 2016 ). In Colombia, previous phylogenetic analysis has shown a major circulation of the BLV Genotype 1 in different departments and mainly in dairy cows ( Benavides et al, 2017 ; Corredor-Figueroa et al, 2020 ; Úsuga-Monroy et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular frequency of bovine leukemia virus in Creole cattle of Eastern Colombia

Jaimes-Dueñez,

Goyeneche-Ortiz,

Tique-Oviedo

et al. 2024

Veterinary and Animal Science

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Section: Discussionmentioning

confidence: 99%

Molecular frequency of bovine leukemia virus in Creole cattle of Eastern Colombia

Jaimes-Dueñez,

Goyeneche-Ortiz,

Tique-Oviedo

et al. 2024

Veterinary and Animal Science

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“…Through these analyses, genotypes 1 and 3 were identified in the samples of the municipalities of Antioquia. Genotype 1 has previously been identified in South America in Brazil, Uruguay, Argentina, and Mexico (in Holstein Friesian Cows) [ 6 , 21 , 23 , 30 ]. Studies have found a correlation between the genotype and the geographical origin of the sample [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of the partial sequences of the env and tax BLV genes reveals the presence of genotypes 1 and 3 in dairy herds of Antioquia, Colombia

Úsuga-Monroy,

Díaz,

González-Herrera

et al. 2023

VirusDis.

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Bovine leukemia virus (BLV) is a retrovirus that primarily infects dairy cows. Although few studies have also used the tax gene, phylogenetic studies of BLV use mostly the env gene. The aim of this work was to establish the circulating genotypes of BLV in specialized dairy cattle from Antioquia, Colombia. Twenty blood samples from Holstein Friesian cows were collected, and their DNA was isolated. A PCR was performed for a partial region of the env and tax genes. A phylogenetic analysis was carried out using the maximum likelihood and Bayesian methods for both genes. Nineteen sequences were identified as genotype 1 by env and tax genes. Only one sequence was clustered with genotype 3 and had the highest proportion of different nucleotide sites compared to other strains. Four amino acid substitutions in the 134 amino acid residue fragment of the Env protein were identified in the Colombian sequences, and three new amino acid substitutions were reported in the 296 amino acid residue fragment of the Tax protein. R43K (Z finger), A185T (Activation domain), and L105F changes were identified in the genotype 3 sample. This genotype has been reported in the United States, Japan, Korea, and Mexico, but so far, not in Colombia. The country has a high rate of imported live animals, semen, and embryos, especially from the United States. Although it is necessary to evaluate samples from other regions of the country, the current results indicate the presence of two BLV genotypes in specialized dairy herds.

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“…Белок R3 состоит из 44 аминокислотных остатков с гидрофильной частью на N-конце и следующим за ней гидрофобным участком. Montero Machuca с соавторами [38] обнаружили 9 несинонимичных мутаций в 5 из 30 исследованных последовательностей вируса BLV генотипа 1, но не выявили их ассоциаций с какими-либо специфическими биологическими эффектами. Результаты опытов с использованием иммунофлуоресценции показали, что после трансляции накопление белка R3 наблюдается в ядре и клеточных мембранах, в то время как G4 локализуется в митохондриях и клеточном ядре.…”

Section: организация генома вируса Blvunclassified

Structural characteristics of the bovine leukemia virus genome: A mini review

Fomenko

2023

Food systems

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Enzootic bovine leukemia is an infectious disease with a chronic course caused by an RNA‑containing virus of the genus Deltaretrovirus. Despite the implementation of various programs for the elimination of leukemia, the disease is still widespread on the planet and continues to cause significant economic damage. A large proportion of BLV‑infected cattle remain to be asymptomatic carriers of the virus, which complicates diagnosis and contributes to the spread of the disease in the herd. The structure of the BLV genome is generally typical of retroviruses. It consists of genes encoding structural proteins, viral enzymes and regulatory elements flanked on both sides by identical long terminal repeats. The enzyme and structural protein coding genes (gag, pro, pol, and env) play a crucial role in the life cycle of the virus, influencing its infectivity and virion production. The tax and rex regulatory genes regulate viral transcription, export of transcripts from the nucleus to the cytoplasm, and disease progression. The increase in the number of copies of proviral DNA occurs mainly not due to the functioning of the virus reverse transcriptase, but because of clonal reproduction of the affected subpopulations of B‑cells, mainly CD5+ IgM+. This feature provides increased genetic stability of the BLV virus. These properties of the viral genome allow the development of a variety of PCR test systems. The widespread implementation of such systems enables the detection of carriers of the disease at early stages, which should contribute to the effective implementation of national programs to eradicate bovine leukemia.

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Genetic analysis of the pX region of bovine leukemia virus genotype 1 in Holstein Friesian cattle with different stages of infection

Cited by 5 publications

References 56 publications

Molecular frequency of bovine leukemia virus in Creole cattle of Eastern Colombia

Molecular frequency of bovine leukemia virus in Creole cattle of Eastern Colombia

Phylogenetic analysis of the partial sequences of the env and tax BLV genes reveals the presence of genotypes 1 and 3 in dairy herds of Antioquia, Colombia

Structural characteristics of the bovine leukemia virus genome: A mini review

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