Genetic analysis of the slow-melting flesh character in peach

Serra, Octávio; Giné-Bordonaba, Jordi; Eduardo, Iban; Bonany, J.; Echeverría, Gemma; Larrigaudière, Christian; Arús, Pere

doi:10.1007/s11295-017-1160-x

Cited by 23 publications

(32 citation statements)

References 36 publications

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“…The activity of an endo-polygalacturonase gene was associated with M flesh [ 48 ]. Recently, Serra et al [ 49 ] identified the qP-MD5 QTL as a key factor for slow M flesh, and qP-MD6 may modulate the maturity date trait. With the development of sequencing and SNP-genotyping platforms, high-resolution linkage maps have been successfully used to locate qualitative and quantitative traits [ 21 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

High-density genetic map construction and quantitative trait loci analysis of the stony hard phenotype in peach based on restriction-site associated DNA sequencing

Guo

Iqbal

et al. 2018

BMC Genomics

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BackgroundPeach (Prunus persica) is an important fruit crop that generally softens rapidly after harvest resulting in a short shelf-life. By contrast, stony hard (SH) peach fruit does not soften and hardly produces ethylene. To explore the candidate genes responsible for the SH phenotype, a high-density genetic map was constructed by restriction-site associated DNA sequencing technology.ResultsIn the present study, the linkage map consisted of 1310 single nucleotide polymorphism markers, spanning 454.2 cM, with an average marker distance of 0.347 cM. The single nucleotide polymorphisms were able to anchor eight linkage groups to their corresponding chromosomes. Based on this high-density integrated peach linkage map and two years of fruit phenotyping, two potential quantitative trait loci for the SH trait were identified and positioned on the genetic map. Additionally, Prupe.6G150900.1, a key gene in abscisic acid (ABA) biosynthesis, displayed a differential expression profile identical to the ABA accumulation pattern: mRNA transcripts were maintained at a high level during storage of SH peaches but occurred at low levels in melting fruit.ConclusionThus Prupe.6G150900.1 might play a crucial role in the SH phenotype of peach in which ABA negatively regulates ethylene production. Also, this high-density linkage map of peach will contribute to the mapping of important fruit traits and quantitative trait loci identification.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4952-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

High-density genetic map construction and quantitative trait loci analysis of the stony hard phenotype in peach based on restriction-site associated DNA sequencing

Guo

Iqbal

et al. 2018

BMC Genomics

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“…In the case of peach, there is an additional advantage: recent results have shown that vast regions of the chromosomes of the modern cultivars (sometimes accounting for 40-60% of the genome) are monomorphic (Donoso et al, 2015;Serra et al, 2017), identity by descent (IBD) being the most logical cause due to a high level of co-ancestry (Micheletti et al, 2015) of peach commercial cultivars. This determines that some of the crossovers produced at the homozygous DNA fragments will not be effective.…”

Section: Genetic Basis Of the Resynthesis Processmentioning

confidence: 99%

Resynthesis: Marker-Based Partial Reconstruction of Elite Genotypes in Clonally-Reproducing Plant Species

et al. 2020

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We propose a method for marker-based selection of cultivars of clonally-reproducing plant species which keeps the basic genetic architecture of a top-performing cultivar (usually a partly heterozygous genotype), with the addition of some agronomically relevant differences (such as production time, product appearance or quality), providing added value to the product or cultivation process. The method is based on selecting a) two complementary nearly-inbred lines from successive selfing generations (ideally only F 2 and F 3) of large size, that may generate individuals with most of their genome identical to the original cultivar but being homozygous for either of the two component haplotypes in the rest, and b) individuals with such characteristics already occurring in the F 2. Option a) allows for introgressing genes from other individuals in one or both of these nearly-inbred lines. Peach, a woody-perennial, clonally-reproduced species, was chosen as a model for a proof of concept of the Resynthesis process due to its biological characteristics: selfcompatibility, compact and genetically well-known genome, low recombination rates and relatively short intergeneration time (3-4 years). From 416 F 2 seedlings from cultivar Sweet Dream (SD), we obtained seven individuals with 76-94% identity with SD, and selected five pairs of complementary lines with average homozygosity of the two parents ≥0.70 such that crossing would produce some individuals highly similar to SD. The application of this scheme to other species with more complex genomes or biological features, including its generalization to F 1 hybrids, is discussed.

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“…In the case of peach, there is an additional advantage: recent results have shown that vast regions of the chromosomes of the modern cultivars (sometimes accounting for 40-60% of the genome) are monomorphic (Serra et al 2017, Donoso et al 2015, identity by descent (IBD) being the most logical cause due to a high level of co-ancestry (Micheletti et al 2015) of peach commercial cultivars. This determines that some of the crossovers produced at the homozygous DNA fragments will not be effective.…”

Section: Genetic Basis Of the Resynthesis Processmentioning

confidence: 99%

Resynthesis: Marker-based partial reconstruction of elite genotypes in clonally-reproducing plant species

Eduardo

Alegre

Alexiou

et al. 2020

Preprint

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We propose a method for marker-based selection of cultivars of clonally-reproducing plant species which keeps the basic genetic architecture of a top-performing cultivar (usually a partly heterozygous genotype), with some agronomically relevant differences (such as production time, product appearance or quality), providing added value to the product or cultivation process. The method is based on selecting a) two complementary nearly-inbred lines from successive selfing generations (ideally only F2 and F3) of large size, that may generate individuals with most of their genome identical to the original cultivar but being homozygous for either of the two component haplotypes in the rest, and b) individuals with such characteristics already occurring in the F2. Option a) allows for introgressing genes from other individuals in one or both of these nearly-inbred lines.Peach, a woody-perennial, clonally-reproduced species, was chosen as a model for a proof of concept of the Resynthesis process due to its biological characteristics: selfcompatibility, compact and genetically well-known genome, low recombination rates and relatively short intergeneration time (3-4 years). From 416 F2 seedlings from cultivar Sweet Dream (SD), we obtained seven individuals with 76-94% identity with SD, and selected five pairs of complementary lines with average homozygosity of the two parents ≥0.70 such that crossing would produce some individuals highly similar to SD. The application of this scheme to other species with more complex genomes or biological features, including its generalization to F1 hybrids, is discussed.

show abstract

Genetic analysis of the slow-melting flesh character in peach

Cited by 23 publications

References 36 publications

High-density genetic map construction and quantitative trait loci analysis of the stony hard phenotype in peach based on restriction-site associated DNA sequencing

High-density genetic map construction and quantitative trait loci analysis of the stony hard phenotype in peach based on restriction-site associated DNA sequencing

Resynthesis: Marker-Based Partial Reconstruction of Elite Genotypes in Clonally-Reproducing Plant Species

Resynthesis: Marker-based partial reconstruction of elite genotypes in clonally-reproducing plant species

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