Genetic analysis of three Amaranth species using ISSR markers

Štefúnová, Veronika; Bežo, Milan; Labajová, Mária; Senková, S.

doi:10.9755/ejfa.v26i1.15911

Cited by 15 publications

(9 citation statements)

References 22 publications

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“…The genotypes of the same species were grouped in the same major cluster (cluster B). Stefunova et al (2014) used ISSR approach to analyze intra-and inter-specific variability of A. caudatus, A. hypochondriacus, and A. cruentus accessions. They reported that most of the accessions of the same species were grouped in the same cluster except some accessions having different origin.…”

Section: Development Of Dendrogrammentioning

confidence: 99%

“…Molecular phylogenetic studies would enable plant breeders to discriminate among genotypes by estimating the extent of variation within and between species which provides a base for appropriate breeding approaches. Well established molecular markerbased techniques such as random amplified polymorphic DNAs (RAPDs) (Faseela and Joseph 2007;Ray and Roy 2008;Popa et al 2010;Snezana et al 2012;Anjali et al 2013), restriction fragment length polymorphisms (RFLPs) (Park et al 2014), simple sequence repeats (SSRs) (Mallory et al 2008;Suresh et al 2014;Wang and Park 2013), amplified fragment length polymorphisms (AFLPs) (Chandi et al 2013), inter-simple sequence repeats (ISSRs) (Nolan et al 2010;Singh et al 2013;Raut et al 2014;Stefunova et al 2014), and single nucleotide polymorphisms (SNPs) (Jimenez et al 2013) have been utilized in genetic diversity studies in Amaranthus species. These molecular markers vary in their level of polymorphism, genomic abundance, reproducibility, locus specificity, dominance or codominance, technical desires, and economic investment (Zietkiewicz et al 1994;Omondi et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…In the last decade, ISSR markers have been successfully utilized for diversity studies. These markers have high reproducibility, which reveal many informative bands in a single amplification, therefore being one of the most commonly used markers in intra-specific diversity analysis in Amaranthus (Nolan et al 2010;Singh et al 2013;Raut et al 2014;Stefunova et al 2014). Hence, the objective of the research presented in this paper was to assess the genetic diversity of 19 genotypes from four different species of Amaranthus of India using ISSR markers to utilize the information generated for breeding and crop improvement.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity analysis and molecular characterization of grain amaranth genotypes using inter simple sequence repeat (ISSR) markers

et al. 2019

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Background: Grain amaranth (Amaranthus spp.) has been cultivated since ancient times in some countries in the world and it is one of the oldest food crops. At present, the crop has gained more importance in the plains of India, especially in parts of Gujarat and Maharashtra. Grain amaranth exhibits an incredible extent of morphological diversity and an extensive adaptability to diverse eco-geographical conditions. Hence, the aim of the recent research was to evaluate the genetic diversity of 19 genotypes from four diverse species of Amaranthus from India using ISSR markers. Results: The set of 11 polymorphic ISSR primers produced a total of 114 amplicons, among which 98 amplicons were polymorphic. The mean number of polymorphic amplicons per primer was 8.91. Overall, the size of PCR-amplified DNA fragments ranged from 200 to 3702 bp. The average percent polymorphism was 87.15%, and the average PIC value was 0.853, which indicates good selection of primers in the present study for the assessment of genetic diversity. The unique amplicon (marker)-producing primers were also found which can be used for identification of genotypes. The dendrogram grouped 19 grain amaranth genotypes into two major clusters. The groups formed on the principle component analysis (PCA) plot resembles with the results of the dendrogram although some genotypes have been diverted on the PCA plot. Conclusions: The technique may be used to obtain reasonably precise information on the genetic relationship among grain amaranth genotypes. Such information may be useful for selecting the diverse parents and monitoring the genetic diversity periodically in the breeder's working collection of grain amaranth.

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Section: Development Of Dendrogrammentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity analysis and molecular characterization of grain amaranth genotypes using inter simple sequence repeat (ISSR) markers

et al. 2019

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“…Morphological evidences have played a significant role in solving taxonomic disputes in amaranths (Costea and DeMason, 2001;Costea and Tardif, 2003b;Das, 2012b). Beside Morphological parameters, biochemical and molecular parameters such as -Isozymes (Chan and Sun, 1997;Hauptli and Jain, 1984;Iduna et al, 2005), Random Amplified Polymorphic DNA (Transue et al, 1994), ISSR, Amplified Fragment Length Polymorphism and ITS (Costea et al, 2006;Nolan et al, 2010;Stefunova et al, 2014;Xu and Sun, 2001) were employed successfully to address the queries regarding taxonomic delimitation and phylogeny in amaranths. In the present investigation besides morphology, isozyme polymorphism of ACP, ISSR fragment polymorphism were employed to trace the relative closeness among the common cultivars of Separate dendrograms on different parameters showed significant concomitance in grouping of taxa with minor variations at the similarity levels or linkage distance.…”

Section: Morphological Studymentioning

confidence: 99%

Initial screening of vegetable amaranth landraces towards extending the vegetable list

Das

2017

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Vegetable amaranth is considered as one of the most favorite vegetables in the world, especially in the hot and humid tropical regions of the globe. Two of them are most popular, i.e., Amaranthus tricolor and Amaranthus blitum, both are represented by a large number of morphotypes or landraces or varieties, which are taxonomically ill-defined, many of them are consumed as palatable vegetables as they look like the conventional cultivars. Due to wide morphological diversity and presence of many synonyms both species are supposed to represent two species complex or aggregates, namely, "Tricolor complex" and "Blitum complex". Two new species have been identified from their landraces. In the present investigation, morphometric analysis along with biochemical and molecular methodologies were applied to explore the relative closeness among few well known, popular vegetables, and few less known landraces for better utilization of the crop biodiversity of vegetable amaranths. The experimental data were statistically analyzed and separate dendrograms were computed on three parameters (morphology, isozyme polymorphism, and inter simple sequence repeat band profile). The members of "Tricolor complex" were clustered together in a single group along with two newly introduced species Amaranthus bengalense (a member of "Blitum complex") and Amaranthus parganensis (a gynomonoecious member of "Tricolor complex"). The "Tricolor complex" represents a plexus of species with varying sexual behavior from gynomonoecy to monoecy and indicates probable origin of monoecious A. tricolor varieties or landraces from gynomonecious member such as A. parganensis as recent introduction. The study indicated a close alliance between A. bengalense, A. parganensis, A. tricolor landrace, and popular A. tricolor varieties which consolidated the feasibility of utilizing the landrace and newly introduced species as potential vegetable.

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“…All markers separated the accessions, but resulted in different cluster structures making further analyses necessary. Another study [45] used ISSRs in diversity analyses of 56 Amaranthus accessions belonging to three species and separated the Amaranthus species based on 11 ISSR primers with only few exceptions.…”

Section: Inter-simple Sequence Repeats (Issrs)mentioning

confidence: 99%

Molecular Markers for Genetic Diversity Studies in African Leafy Vegetables

Omondi¹,

Debener²,

Linde³

et al. 2016

ABB

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African leafy vegetables are becoming important crops in tackling nutrition and food security in many parts of sub-Saharan Africa, since they provide important micronutrients and vitamins, and help resource-poor farm families bridge lean periods of food shortage. Genetic diversity studies are essential for crop improvement programmes as well as germplasm conservation efforts, and research on genetic diversity of these vegetables using molecular markers has been increasing over time. Diversity studies have evolved from the use of morphological and biochemical markers to molecular markers. Molecular markers provide valuable data, since they detect mostly selectively neutral variations at the DNA level. They are well established and their strengths and limitations have been described. New marker types are being developed from a combination of the strengths of the basic techniques to improve sensitivity, reproducibility, polymorphic information content, speed and cost. This review discusses the principles of some of the established molecular markers and their application to genetic diversity studies of African leafy vegetables with a main focus on the most common Solanum, Amaranthus, Cleome and Vigna species.

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Genetic analysis of three Amaranth species using ISSR markers

Cited by 15 publications

References 22 publications

Genetic diversity analysis and molecular characterization of grain amaranth genotypes using inter simple sequence repeat (ISSR) markers

Genetic diversity analysis and molecular characterization of grain amaranth genotypes using inter simple sequence repeat (ISSR) markers

Initial screening of vegetable amaranth landraces towards extending the vegetable list

Molecular Markers for Genetic Diversity Studies in African Leafy Vegetables

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