Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test

Moraes, Marcia Terezinha Baroni de; Silva, Messias da; Leite, José Paulo Gagliardi; Nascimento, Jussara Pereira do

doi:10.1590/s0074-02761998000200019

Cited by 5 publications

(2 citation statements)

References 25 publications

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“…In this outbreak, type 3 illness rates may have peaked and begun a natural decline before reintroduction of vaccines, but rates continued to decrease dramatically after vaccination was restarted. Although serotype 7 vaccine should not prevent serotype 3 illness, and although antiserum samples generally are not crossreactive, some antigenic similarity between serotype 3 and serotype 7 pathogens has been suggested [43], and it could explain this observation.…”

Section: Discussionmentioning

confidence: 99%

Large Epidemic of Respiratory Illness Due to Adenovirus Types 7 and 3 in Healthy Young Adults

Ryan

Gray

Smith

et al. 2002

Clinical Infectious Diseases

122

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After 25 years of successful control through immunization, respiratory infections due to adenoviruses have reemerged to threaten the health of young adults in the military. Shortly after the loss of adenovirus vaccine supplies, a large outbreak of respiratory illness was observed at the United States Navy's sole basic training center. Laboratory testing confirmed 541 cases of adenovirus infection, including 378 cases due to serotype 7 and 132 cases due to serotype 3. This outbreak was remarkable because of its unique serotype distribution and the large amount of data available to describe demographic factors associated with infection. This was the largest outbreak of respiratory illness due to adenovirus types 7 and 3 documented in recent history, and it portends even greater challenges for young adults in the military in the postvaccine era.

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Section: Discussionmentioning

confidence: 99%

Large Epidemic of Respiratory Illness Due to Adenovirus Types 7 and 3 in Healthy Young Adults

Ryan

Gray

Smith

et al. 2002

Clinical Infectious Diseases

122

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“…The hexon protein is the major capsid protein and consists of conserved pedestal regions flanking variable loops (Raue & Hess 1989). The L1 loops at the top of the hexon molecule form the outer surface of the virion and contain several important groupspecific and subgroup-specific epitopes (Norrby 1969) with high-sequence variability between the different FAdVs (Morães et al 1998). Nucleic acid sequences of these typespecific epitopes have been used as phylogenetic markers to establish the relationship between European and American aviadenoviruses (Meulemans et al 2004).…”

Section: The Genus Aviadenovirus Is Further Divided Into Eight Speciementioning

confidence: 99%

Molecular differentiation and pathogenicity of Aviadenoviruses isolated during an outbreak of inclusion body hepatitis in South Africa

Joubert¹,

Aitchison²,

Maartens³

et al. 2014

J S Afr Vet Assoc

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Fowl adenovirus (FAdV) is a member of the genus Aviadenovirus and causes a number of economically important poultry diseases. One of these diseases, inclusion body hepatitis (IBH), has a worldwide distribution and is characterised by acute mortality (5% - 20%) in production chickens. The disease was first described in the United States of America in 1963 and has also been reported in Canada, the United Kingdom, Australia, France and Ireland, but until now, not in South Africa. Adenoviruses isolated from the first outbreak of IBH in South Africa were able to reproduce the disease in chicken embryo livers. The aim of the present study was to characterise the viruses and determine the pathogenicity of the FAdV strains responsible for the first reported case of IBH in South Africa. Polymerase chain reaction (PCR) amplification of the L1 loop region of the fowl adenovirus hexon gene using degenerate primer pair hexon A/B was used to identify the viruses that were isolated. Restriction fragment length polymorphism (RFLP) of the amplification products was used for the differentiation of 14 isolates of fowl adenovirus. Sequencing of the PCR products followed by amino acid comparison and phylogenetic analysis using the L1 loop region of the hexon protein was done to determine the identity of the isolates. Amino acid sequences of the hexon genes of all the South African isolates were compared with those of reference strains representing FAdV species. Amino acid comparison of 12 South Africa field isolates to FAdV reference strains revealed a high sequence identity (> 93.33%) with reference strains T8-A and 764. Two of the isolates had high sequence identity (93.40%) with reference strains P7-A, C2B and SR48. Phylogenetic analysis of the L1 loop region of the hexon protein of all 14 South African isolates was consistent with their RFLP clusters. The mortality rates of embryos challenged with 106 egg infective doses (EID50) FAdV 2 were 80% - 87% and mortality rates for embryos challenged with 105.95 (EID50) FAdV 8b were 65% - 80%.

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Adenoviruses: Biology and Epidemiology

Kajon

González

2017

Human Virology in Latin America

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Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test

Abstract: During an epidemiological survey of acute respiratory infection in Rio de

Cited by 5 publications

References 25 publications

Large Epidemic of Respiratory Illness Due to Adenovirus Types 7 and 3 in Healthy Young Adults

Large Epidemic of Respiratory Illness Due to Adenovirus Types 7 and 3 in Healthy Young Adults

Molecular differentiation and pathogenicity of Aviadenoviruses isolated during an outbreak of inclusion body hepatitis in South Africa

Adenoviruses: Biology and Epidemiology

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